Q8AXY1 · PA2A_BOTJR

Function

function

Snake venom phospholipase A2 (PLA2) that displays edema-inducing activities (activity that is inhibited by EDTA and dexamethasone), inhibits phospholipid-dependent collagen/ADP-induced platelet aggregation, possess hypotensive as well as anticoagulant activities. In addition, this enzyme shows bactericidal activity against E.coli and S.aureus. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Miscellaneous

This enzyme has been found to be not myotoxic, not cytotoxic, not hemorrhagic and not lethal.

Catalytic activity

Cofactor

Ca2+ (UniProtKB | Rhea| CHEBI:29108 )

Note: Binds 1 Ca2+ ion per subunit.

Activity regulation

Inhibited by EDTA and bromophenacyl bromide (BPB).

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site43Ca2+ (UniProtKB | ChEBI)
Binding site47Ca2+ (UniProtKB | ChEBI)
Binding site48Ca2+ (UniProtKB | ChEBI)
Active site63
Binding site64Ca2+ (UniProtKB | ChEBI)
Active site105

GO annotations

AspectTerm
Cellular Componentextracellular region
Molecular Functioncalcium ion binding
Molecular Functioncalcium-dependent phospholipase A2 activity
Molecular Functionphospholipid binding
Molecular Functiontoxin activity
Biological Processarachidonic acid secretion
Biological Processdefense response to bacterium
Biological Processlipid catabolic process
Biological Processphospholipid metabolic process
Biological Processregulation of blood pressure

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Acidic phospholipase A2 BthA-1
  • EC number
  • Short names
    svPLA2
  • Alternative names
    • BOJU-III
    • BthA-I-PLA2
    • Hypotensive phospholipase A2
    • Phosphatidylcholine 2-acylhydrolase

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Lepidosauria > Squamata > Bifurcata > Unidentata > Episquamata > Toxicofera > Serpentes > Colubroidea > Viperidae > Crotalinae > Bothrops

Accessions

  • Primary accession
    Q8AXY1

Subcellular Location

Keywords

Phenotypes & Variants

Toxic dose

LD50 is 25 mg/kg by intraperitoneal injection into mice.
LD50 is 7.5 mg/kg by intravenous injection into mice.
LD50 is 0.5 mg/kg by intracerebroventricular injection.

PTM/Processing

Features

Showing features for signal, chain, disulfide bond.

TypeIDPosition(s)Description
Signal1-16
ChainPRO_000041380417-138Acidic phospholipase A2 BthA-1
Disulfide bond42↔131
Disulfide bond44↔60
Disulfide bond59↔111
Disulfide bond65↔138
Disulfide bond66↔104
Disulfide bond73↔97
Disulfide bond91↔102

Keywords

Expression

Tissue specificity

Expressed by the venom gland.

Interaction

Subunit

Homodimer; non-covalently linked.

Family & Domains

Sequence similarities

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    138
  • Mass (Da)
    15,456
  • Last updated
    2003-03-01 v1
  • Checksum
    513647907BFD0F4E
MRTLWIMAVLLVGVEGSLWQFGKMINYVMGESGVLQYLSYGCYCGLGGQGQPTDATDRCCFVHDCCYGKVTGCDPKIDSYTYSKKNGDVVCGGDDPCKKQICECDRVATTCFRDNKDTYDIKYWFYGAKNCQEKSEPC

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY145836
EMBL· GenBank· DDBJ
AAN37410.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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