Q89A49 · LEU1_BUCBP
- ProteinPutative 2-isopropylmalate synthase
- GeneleuA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids390 (go to sequence)
- Protein existenceUncertain
- Annotation score3/5
Function
function
Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-ketoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).
Catalytic activity
- 3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H+
Cofactor
Pathway
Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 2-isopropylmalate synthase activity | |
Molecular Function | metal ion binding | |
Biological Process | L-leucine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePutative 2-isopropylmalate synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Erwiniaceae > Buchnera
Accessions
- Primary accessionQ89A49
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000140336 | 1-390 | Putative 2-isopropylmalate synthase | |||
Sequence: MTQKIIIFDTTLRDGEQSLKMSLSVKKKLKIAFALEKLGVDVIEAGFPISSPGDFESVKKISERIKDAKICSLARCIDGDIDIAAKAMKKANSFRIHIFLGTSALHVQSKLKKTFDQIIDMMVSSVKRAQKYTDDVEFSCEDAGRTSLDDLCRIIELAIDLGVKTINIPDTVGYTIPYEFSNIISSIYKKVPNIDKAIISVHCHDDLGMAVANSISAIQVGARQIEGTITGVGERAGNAALEEILMAIKIRKDILNFKTNIKYQEIYSTCRVISSICNIPVPVNKAIIGSNAFSHSSGIHQDGILKDKKTYEIIVPESIGFVSQPLNLTSRSGRAAVKYRMKKIGYKDSDYNIDILYSRFLKLADKKGRVSDSDLKQLVCFNNKLKNLKD |
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 5-267 | Pyruvate carboxyltransferase | ||||
Sequence: IIIFDTTLRDGEQSLKMSLSVKKKLKIAFALEKLGVDVIEAGFPISSPGDFESVKKISERIKDAKICSLARCIDGDIDIAAKAMKKANSFRIHIFLGTSALHVQSKLKKTFDQIIDMMVSSVKRAQKYTDDVEFSCEDAGRTSLDDLCRIIELAIDLGVKTINIPDTVGYTIPYEFSNIISSIYKKVPNIDKAIISVHCHDDLGMAVANSISAIQVGARQIEGTITGVGERAGNAALEEILMAIKIRKDILNFKTNIKYQEIY |
Sequence similarities
Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length390
- Mass (Da)43,227
- Last updated2003-06-01 v1
- Checksum780EA1D8D08D2B07
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE016826 EMBL· GenBank· DDBJ | AAO27198.1 EMBL· GenBank· DDBJ | Genomic DNA |