Q88KX0 · DUSA_PSEPK
- ProteintRNA-dihydrouridine(20/20a) synthase
- GenedusA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids336 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.
Catalytic activity
- 5,6-dihydrouridine20 in tRNA + NADP+ = H+ + NADPH + uridine20 in tRNA
- 5,6-dihydrouridine(20a) in tRNA + NADP+ = H+ + NADPH + uridine(20a) in tRNA
- 5,6-dihydrouridine(20a) in tRNA + NAD+ = H+ + NADH + uridine(20a) in tRNA
Cofactor
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 24-26 | FMN (UniProtKB | ChEBI) | ||||
Sequence: PMM | ||||||
Binding site | 77 | FMN (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Site | 104 | Interacts with tRNA | ||||
Sequence: N | ||||||
Active site | 107 | Proton donor | ||||
Sequence: C | ||||||
Binding site | 146 | FMN (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 178 | FMN (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Site | 190 | Interacts with tRNA; defines subfamily-specific binding signature | ||||
Sequence: K | ||||||
Site | 193 | Interacts with tRNA | ||||
Sequence: R | ||||||
Binding site | 218-220 | FMN (UniProtKB | ChEBI) | ||||
Sequence: NGG | ||||||
Binding site | 240-241 | FMN (UniProtKB | ChEBI) | ||||
Sequence: GR | ||||||
Site | 306 | Interacts with tRNA; defines subfamily-specific binding signature | ||||
Sequence: R | ||||||
Site | 309 | Interacts with tRNA; defines subfamily-specific binding signature | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-dihydrouridine20 synthase activity | |
Molecular Function | tRNA-dihydrouridine20a synthase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-dihydrouridine(20/20a) synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionQ88KX0
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000162070 | 1-336 | tRNA-dihydrouridine(20/20a) synthase | |||
Sequence: MSLETASTPHTTRPEPSRRFSVAPMMDWTDRHCRFFLRLLSRQTLLYTEMVTTGALLHNDAHRFLRHDASEHPLALQLGGSVPADLAACARLAEEAGYDEVNLNVGCPSDRVQNNMIGACLMAHPALVADCVKAMRDAVSTPVTVKHRIGINGRDSYAELSDFVGQVREAGCRSFTVHARIAILEGLSPKENREIPPLRYDIAAQLKRDFPDLEIVLNGGIKTLDECQAHLETFDGVMLGREAYHNPYLLAEVDQQLFGSDAPVVSRSEALAQLRPYIVAHMESGGAMHHVTRHILGLAQGFKGARRFRQLLSADIHKAAEPLAVFDQAVELLQGR |
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length336
- Mass (Da)37,130
- Last updated2003-06-01 v1
- ChecksumEB57745ACD43EC21
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE015451 EMBL· GenBank· DDBJ | AAN67782.1 EMBL· GenBank· DDBJ | Genomic DNA |