Q88KX0 · DUSA_PSEPK

Function

function

Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.

Catalytic activity

Cofactor

FMN (UniProtKB | Rhea| CHEBI:58210 )

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site24-26FMN (UniProtKB | ChEBI)
Binding site77FMN (UniProtKB | ChEBI)
Site104Interacts with tRNA
Active site107Proton donor
Binding site146FMN (UniProtKB | ChEBI)
Binding site178FMN (UniProtKB | ChEBI)
Site190Interacts with tRNA; defines subfamily-specific binding signature
Site193Interacts with tRNA
Binding site218-220FMN (UniProtKB | ChEBI)
Binding site240-241FMN (UniProtKB | ChEBI)
Site306Interacts with tRNA; defines subfamily-specific binding signature
Site309Interacts with tRNA; defines subfamily-specific binding signature

GO annotations

AspectTerm
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular FunctiontRNA binding
Molecular FunctiontRNA-dihydrouridine20 synthase activity
Molecular FunctiontRNA-dihydrouridine20a synthase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-dihydrouridine(20/20a) synthase
  • EC number
  • Alternative names
    • U20-specific dihydrouridine synthase
      (U20-specific Dus
      )
    • tRNA-dihydrouridine synthase A

Gene names

    • Name
      dusA
    • Ordered locus names
      PP_2169

Organism names

Accessions

  • Primary accession
    Q88KX0

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001620701-336tRNA-dihydrouridine(20/20a) synthase

Proteomic databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the Dus family. DusA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    336
  • Mass (Da)
    37,130
  • Last updated
    2003-06-01 v1
  • Checksum
    EB57745ACD43EC21
MSLETASTPHTTRPEPSRRFSVAPMMDWTDRHCRFFLRLLSRQTLLYTEMVTTGALLHNDAHRFLRHDASEHPLALQLGGSVPADLAACARLAEEAGYDEVNLNVGCPSDRVQNNMIGACLMAHPALVADCVKAMRDAVSTPVTVKHRIGINGRDSYAELSDFVGQVREAGCRSFTVHARIAILEGLSPKENREIPPLRYDIAAQLKRDFPDLEIVLNGGIKTLDECQAHLETFDGVMLGREAYHNPYLLAEVDQQLFGSDAPVVSRSEALAQLRPYIVAHMESGGAMHHVTRHILGLAQGFKGARRFRQLLSADIHKAAEPLAVFDQAVELLQGR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE015451
EMBL· GenBank· DDBJ
AAN67782.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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