Q88KV1 · MIAE_PSEPK
- ProteintRNA 2-(methylsulfanyl)-N(6)-isopentenyladenosine(37) hydroxylase
- GenemiaE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids205 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in specific tRNA modification. Catalyzes the oxygen-dependent hydroxylation of 2-methylthio-N-6-isopentenyl adenosine (ms2i6A) to produce 2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine (ms2io6A) at position 37 in tRNAs.
Catalytic activity
- 2-methylsulfanyl-N6-dimethylallyladenosine37 in tRNA + AH2 + O2 = N6-[(2E)-4-hydroxy-3-methylbut-2-en-1-yl]-2-(methylsulfanyl)adenosine37 in tRNA + A + H2OThis reaction proceeds in the forward direction.
Cofactor
Note: Contains a nonheme dinuclear iron cluster.
Pathway
tRNA modification; 2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine-tRNA biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 38 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 69 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 69 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 72 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 122 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 151 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 154 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | metal ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine)-hydroxylase activity | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA 2-(methylsulfanyl)-N(6)-isopentenyladenosine(37) hydroxylase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionQ88KV1
Proteomes
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 40 | Loss of activity. | ||||
Sequence: K → D | ||||||
Mutagenesis | 100 | Loss of activity. | ||||
Sequence: R → E |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000454384 | 1-205 | tRNA 2-(methylsulfanyl)-N6-isopentenyladenosine37 hydroxylase | |||
Sequence: MSLIPEIDAFLGCPTPDAWIEAALADQETLLIDHKNCEFKAASTALSLIAKYNTHLDLINMMSRLAREELVHHEQVLRLMKRRGVPLRPVSAGRYASGLRRLVRAHEPVKLVDTLVVGAFIEARSCERFAALVPHLDEELGRFYHGLLKSEARHYQGYLKLAHNYGDEADIARCVELVRAAEMELIQSPDQELRFHSGIPQALAA |
Proteomic databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length205
- Mass (Da)23,031
- Last updated2003-06-01 v1
- ChecksumDA044B40D55EA197
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE015451 EMBL· GenBank· DDBJ | AAN67801.1 EMBL· GenBank· DDBJ | Genomic DNA |