Q88KV1 · MIAE_PSEPK

Function

function

Involved in specific tRNA modification. Catalyzes the oxygen-dependent hydroxylation of 2-methylthio-N-6-isopentenyl adenosine (ms2i6A) to produce 2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine (ms2io6A) at position 37 in tRNAs.

Catalytic activity

Cofactor

Fe cation (UniProtKB | Rhea| CHEBI:24875 )

Note: Contains a nonheme dinuclear iron cluster.

Pathway

tRNA modification; 2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine-tRNA biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site38Fe cation 1 (UniProtKB | ChEBI)
Binding site69Fe cation 1 (UniProtKB | ChEBI)
Binding site69Fe cation 2 (UniProtKB | ChEBI)
Binding site72Fe cation 1 (UniProtKB | ChEBI)
Binding site122Fe cation 2 (UniProtKB | ChEBI)
Binding site151Fe cation 2 (UniProtKB | ChEBI)
Binding site154Fe cation 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionmetal ion binding
Molecular Functionmonooxygenase activity
Molecular FunctiontRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine)-hydroxylase activity
Biological ProcesstRNA modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA 2-(methylsulfanyl)-N(6)-isopentenyladenosine(37) hydroxylase
  • EC number
  • Alternative names
    • 2-methylthio-N6-isopentenyladenosine(37)-tRNA monooxygenase
    • tRNA-(ms[2]io[6]A37)-hydroxylase

Gene names

    • Name
      miaE
    • Ordered locus names
      PP_2188

Organism names

Accessions

  • Primary accession
    Q88KV1

Proteomes

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis40Loss of activity.
Mutagenesis100Loss of activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004543841-205tRNA 2-(methylsulfanyl)-N6-isopentenyladenosine37 hydroxylase

Proteomic databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the MiaE family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    205
  • Mass (Da)
    23,031
  • Last updated
    2003-06-01 v1
  • Checksum
    DA044B40D55EA197
MSLIPEIDAFLGCPTPDAWIEAALADQETLLIDHKNCEFKAASTALSLIAKYNTHLDLINMMSRLAREELVHHEQVLRLMKRRGVPLRPVSAGRYASGLRRLVRAHEPVKLVDTLVVGAFIEARSCERFAALVPHLDEELGRFYHGLLKSEARHYQGYLKLAHNYGDEADIARCVELVRAAEMELIQSPDQELRFHSGIPQALAA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE015451
EMBL· GenBank· DDBJ
AAN67801.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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