Q88JX5 · GALA_PSEPK
- ProteinGallate dioxygenase
- GenegalA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids340 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Ring-cleavage dioxygenase that acts specifically on gallate to produce the keto-tautomer of 4-oxalomesaconate. Mediates the first step of gallate degradation pathway.
Catalytic activity
- 3,4,5-trihydroxybenzoate + O2 = (1E)-4-oxobut-1-ene-1,2,4-tricarboxylate + 2 H+
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
144 μM | gallate |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
53.2 μmol/min/mg | with gallate as substrate |
pH Dependence
Optimum pH is 7.0.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 45 | Proton donor | ||||
Sequence: H | ||||||
Active site | 113 | Proton acceptor | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ferrous iron binding | |
Molecular Function | gallate dioxygenase activity | |
Biological Process | gallate catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGallate dioxygenase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionQ88JX5
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000418495 | 1-340 | Gallate dioxygenase | |||
Sequence: MADEGGNPRDLPPVGGHAALSRHIGQSLMADEFDMSFFRDKPLDHGFFSPMSALLPCDESWPVQIVPLQVGVLQLPIPTARRCYKLGQALRRAIESYPEDLKVAIVATGGVSHQVHGERCGFNNPEWDAQFLDLLVNDPQRLTEMTLAEYATLGGMEGAEVITWLIMRGTLSANVERKHQSYYLPSMTGIATLLLENRDQALPAPVNERHRQHMQHQLAGAEQLEGTYPYTLERSAKGYRLNKFLHRMIEPQWRQRFLSEPEALYREAGLSEEESDLLRRRDWRGLIHYGVIFFVLEKLGAVLGVSNLDIYAAMRGQSIEDFMKTRNQQVRYSVAGKAPN |
Proteomic databases
Expression
Induction
By gallate.
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length340
- Mass (Da)38,459
- Last updated2003-06-01 v1
- ChecksumAB4C6FEF2021E56C
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE015451 EMBL· GenBank· DDBJ | AAN68130.1 EMBL· GenBank· DDBJ | Genomic DNA |