Q88CC1 · HGLS_PSEPK

Function

function

Catalyzes the decarboxylation and hydroxylation of 2-oxoadipate (2OA) to form D-2-hydroxyglutarate (D-2-HGA) (PubMed:31064836, PubMed:32523014).
Is specific for 2-oxoadipate (PubMed:32523014).
Is involved in a D-lysine catabolic pathway (PubMed:31064836).

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Activity regulation

Inhibited by EDTA.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.06 mM2-oxoadipate
0.01 mM2-oxoadipate
kcat is 330 min-1.

Pathway

Amino-acid degradation.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site702-oxoadipate (UniProtKB | ChEBI)
Binding site70Fe2+ (UniProtKB | ChEBI)
Binding site742-oxoadipate (UniProtKB | ChEBI)
Site74Important for substrate specificity
Binding site2262-oxoadipate (UniProtKB | ChEBI)
Binding site226Fe2+ (UniProtKB | ChEBI)
Binding site294Fe2+ (UniProtKB | ChEBI)
Binding site4022-oxoadipate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functiondioxygenase activity

Keywords

Enzyme and pathway databases

Community curation (1)

Community annotation

Catalyzes the intramolecular decarboxylation/hydroxylation of 2-oxoadipate to D-2-hydroxyglutarate.

SourceSubmission dateContributor
PubMed:310648360000-0002-1490-8074

Names & Taxonomy

Protein names

  • Recommended name
    2-oxoadipate dioxygenase/decarboxylase
  • EC number
  • Alternative names
    • 2-hydroxyglutarate synthase

Gene names

    • Name
      hglS
    • Synonyms
      ydcJ
    • Ordered locus names
      PP_5260
Community curation (1)

Community suggested name: Hydroxyglutarate synthase.

SourceSubmission dateContributor
PubMed:310648360000-0002-1490-8074

Organism names

Accessions

  • Primary accession
    Q88CC1

Proteomes

Phenotypes & Variants

Disruption phenotype

Deletion mutant is unable to grow on either isomer of lysine.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis74Loss of activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004577821-4642-oxoadipate dioxygenase/decarboxylase

Proteomic databases

Expression

Induction

Up-regulated when grown on L-lysine, D-lysine or 2-aminoadipate.

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    464
  • Mass (Da)
    51,372
  • Last updated
    2003-06-01 v1
  • Checksum
    59A237FBD2E79FDC
MPANDFVSPDSIRAQFSAAMSLMYKQEVPLYGTLLELVSEINQQVMAQQPEVAEALRWTGEIERLDQERHGAIRVGTAEELATIARLFAVMGMQPVGYYDLSSAGVPVHSTAFRAVHEQSLHVSPFRVFTSLLRLELIDNPQLRELAQSILAKRQIFTSRALELIAQCEREGGLDAADAETFVQEALHTFRWHQDATVTAEQYQQLHDQHRLIADVVAFKGPHINHLTPRTLDIDAIQLGMPAKGIPPKAVVEGPPTRRHPILLRQTSFKALQETVAFRDQQGREGSHTARFGEIEQRGAALTPKGRQLYDKLLDATRVALGGAPAEANAERYMALLQANFAEFPDDLAQMREQGLAYFRYFATEKGLAARDQEGRPTTLQGLIDAGHVHFEALVYEDFLPVSAAGIFQSNLGDDAQAEYGSNANREAFEAALGLQVQDELALYAQSERRSLQACAQALNLGSM

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE015451
EMBL· GenBank· DDBJ
AAN70825.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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