Q889E1 · ISPH_PSESM

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site12[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site41(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site41dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site41isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site74(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site74dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site74isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site96[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site124(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site124dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site124isopentenyl diphosphate (UniProtKB | ChEBI)
Active site126Proton donor
Binding site168(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site198[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site226(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site226dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site226isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site227(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site227dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site227isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site228(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site228dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site228isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site270(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site270dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site270isopentenyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • Ordered locus names
      PSPTO_0809

Organism names

Accessions

  • Primary accession
    Q889E1

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001288601-3154-hydroxy-3-methylbut-2-enyl diphosphate reductase

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the IspH family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    315
  • Mass (Da)
    34,384
  • Last updated
    2003-06-01 v1
  • Checksum
    360DD2DBC08E65A9
MQIKLANPRGFCAGVDRAIEIVNRALEVFGPPIYVRHEVVHNKFVVEDLRSRGAIFVEELEQVPDDVIVIFSAHGVSQAVRTEAAGRGLKVFDATCPLVTKVHIEVARYSRDGRECILIGHAGHPEVEGTMGQYDAVNGGAIYLVEDEADVASLEVRNPESLAFVTQTTLSMDDTSRVIDALRKRFPAIGGPRKDDICYATQNRQDAVKQLADECDVVLVVGSPNSSNSNRLRELAERMATPAYLIDGAEDMQKGWFDGVERIGITAGASAPEVLVRGVIQQLQAWGATGADELAGREENITFSMPKELRVKSLL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE016853
EMBL· GenBank· DDBJ
AAO54351.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help