Q87A48 · Q87A48_XYLFT

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

111321002003004005006007008009001,0001,100
TypeIDPosition(s)Description
Binding site301Mg2+ (UniProtKB | ChEBI); catalytic
Binding site344Mg2+ (UniProtKB | ChEBI); catalytic
Binding site402Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site405Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • Ordered locus names
      PD_1983

Organism names

Accessions

  • Primary accession
    Q87A48

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain39-118S1 motif
Region402-405Required for zinc-mediated homotetramerization and catalytic activity
Compositional bias575-597Polar residues
Region575-703Disordered
Compositional bias607-623Polar residues
Compositional bias631-670Polar residues
Compositional bias678-697Polar residues
Compositional bias730-788Polar residues
Region730-841Disordered
Compositional bias811-839Polar residues
Compositional bias900-931Polar residues
Region900-1011Disordered
Compositional bias939-964Polar residues
Compositional bias976-1011Polar residues
Region1087-1132Disordered
Compositional bias1094-1132Polar residues

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,132
  • Mass (Da)
    126,151
  • Last updated
    2003-06-01 v1
  • Checksum
    AA5010FD768E01FE
MKRMLINATQAEELRVAIVDGQSLYDIDIEQPSKEQKKSNIYKGRITRLEPSLEAAFVDYGTDRHGFLPLKEISKDYFQAGLDHHKAGIRELLREGQEIVVQVDKEERGNKGAALTTFISLAGRYMVLMPNSPSAGGVSRRIEGEDRAALKEALEKLNIPNDMGVIIRTAGVGRDTQELQWDLDFLLHAWRAIAEAALSKPAPLLIYQESRLIIRALRDYFRADIGEILVDTEEMYQDAHQFMRHVMPQSLRKLKYYKDDIPLFNRFQIESQIEGAYERNVRLPSGGSIVVDQTEALTAIDVNSSRATKGNDIEETAFQTNLEAADEIARQLRLRDVGGLVVIDFIDMSSSKHQREIETRLQNCLKYDRARVQLSRISRFGLLEMSRQRLRPSLGESSQIVCPYCDGHGRMRSIESLSLSIIRIVEEHAMKESTGQVLVQTPVEIANYLLNEKRRAINEIEKRHDAPIIIIADEQLHTPHYEVTRLRENELSEETGKPSYQRNTPRKLPVHALTKSQLNIPPAPAVKSITPPQLAPTQENIPTIREQSAENRAHPIPELVSSLASWLKRIFGNTDKRQTASNSSHPAPLQQNRNGNVQRNERNRRDGQIATSNTQPQQQSSSHRDNYRHNESNNYPQIQNQCTADTNGKLRNESQTPKSSAYVSKLPQMPRQPQPQLPKTPRTQSQPTQNSTKTILDKQPAPVLHGEDNTLQKAKPIPLTAEAVLNNIPAPSMTATPSGKDQDALPTTSNVTQGTSNALIEAKSTTKTNINTNTDIEPQQNNHEGTGNNDGSNRRRRGRRGGRRRRRNTSLGNETNAVSESNSIKLSNDTETDNADNIESPQALRPAMQENKRQPEFEFDDLAPAAPITAPLRKAIAAEREGISADLTTTAPQSIMETASRTHIASQMAPQSETSAASTTPNASVQATKTHTHKDVVSDSPSRTPYNAHSNTTRHSSGRRLETLSTAHPSAKAPPSEHITPTSRSNANTKTTKTEIPNKPTNANSDTAARPTTTDVLVVTNQPVIATKPSTTIEKAQTKVIPSTTYNNEHIETKRKIVTTESETTQPPVRKQRLPMQNPLPKVVAPLEDKKQAPFVENTTQRNINNATTTSQNDKSLGFTKNNEENSDNNTL

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias575-597Polar residues
Compositional bias607-623Polar residues
Compositional bias631-670Polar residues
Compositional bias678-697Polar residues
Compositional bias730-788Polar residues
Compositional bias811-839Polar residues
Compositional bias900-931Polar residues
Compositional bias939-964Polar residues
Compositional bias976-1011Polar residues
Compositional bias1094-1132Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE009442
EMBL· GenBank· DDBJ
AAO29812.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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