Q874B9 · EF2_PICPA
- ProteinElongation factor 2
- GeneEFT1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids842 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Catalytic activity
- GTP + H2O = GDP + phosphate + H+This reaction proceeds in the forward direction.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 26-33 | GTP (UniProtKB | ChEBI) | |||
Binding site | 158-161 | GTP (UniProtKB | ChEBI) | |||
Binding site | 213-215 | GTP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | ribonucleoprotein complex | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | ribosome binding | |
Molecular Function | translation elongation factor activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameElongation factor 2
- EC number
- Short namesEF-2
Gene names
Organism names
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Pichiaceae > Komagataella
Accessions
- Primary accessionQ874B9
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000091020 | 1-842 | Elongation factor 2 | ||
Modified residue | 699 | Diphthamide | |||
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 17-253 | tr-type G | |||
Sequence similarities
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length842
- Mass (Da)93,469
- Last updated2003-06-01 v1
- ChecksumBED5CF616E47F1A6
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY219033 EMBL· GenBank· DDBJ | AAO39212.1 EMBL· GenBank· DDBJ | Genomic DNA |