Q84WV6 · MORC1_ARATH
- ProteinProtein MICRORCHIDIA 1
- GeneMORC1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids635 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Mediator of defense signaling triggered by distinct classes of R proteins. Required during hypersensitive response (HR) that confers disease resistance to turnip crinkle virus (TCV). Exhibits ATPase activity (PubMed:18191794, PubMed:19704828).
Contributes to resistance against Pseudomonas syringae and Hyaloperonospora arabidopsidis, at early stages prior to cytosolic calcium ions Ca2+ accumulation (PubMed:20332379).
Required for pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI), basal resistance, non-host resistance and systemic acquired resistance (SAR). Binds DNA/RNA in a non-specific manner and exhibits endonuclease activity. Probably involved in DNA repair (PubMed:23250427).
Required for both RPP8- and SSI4-mediated resistance responses, thus being involved in both TIR- and CC-NB-LRR pathways (PubMed:18191794).
Involved in RNA-directed DNA methylation (RdDM) as a component of the RdDM machinery and required for gene silencing (PubMed:24799676).
May also be involved in the regulation of chromatin architecture to maintain gene silencing (PubMed:22555433, PubMed:24799676).
Contributes to resistance against Pseudomonas syringae and Hyaloperonospora arabidopsidis, at early stages prior to cytosolic calcium ions Ca2+ accumulation (PubMed:20332379).
Required for pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI), basal resistance, non-host resistance and systemic acquired resistance (SAR). Binds DNA/RNA in a non-specific manner and exhibits endonuclease activity. Probably involved in DNA repair (PubMed:23250427).
Required for both RPP8- and SSI4-mediated resistance responses, thus being involved in both TIR- and CC-NB-LRR pathways (PubMed:18191794).
Involved in RNA-directed DNA methylation (RdDM) as a component of the RdDM machinery and required for gene silencing (PubMed:24799676).
May also be involved in the regulation of chromatin architecture to maintain gene silencing (PubMed:22555433, PubMed:24799676).
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Uses preferentially Mn2+ and, to a lesser extent, Mg2+ as cofactors.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endosome | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Molecular Function | endonuclease activity | |
Molecular Function | RNA binding | |
Biological Process | chromatin organization | |
Biological Process | defense response to virus | |
Biological Process | DNA repair | |
Biological Process | plant-type hypersensitive response | |
Biological Process | positive regulation of defense response to bacterium | |
Biological Process | positive regulation of defense response to virus by host | |
Biological Process | positive regulation of plant-type hypersensitive response | |
Biological Process | positive regulation of systemic acquired resistance | |
Biological Process | regulation of DNA repair | |
Biological Process | regulatory ncRNA-mediated gene silencing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein MICRORCHIDIA 1
- EC number
- Short namesAtMORC1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ84WV6
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Present in nuclear bodies near chromocenters (PubMed:22555433).
Localized in endosome-like vesicles displaying rapid cytosolic streaming (PubMed:20332379).
Accumulates in the nucleus following pathogen-associated molecular pattern (PAMP) treatment or infection with an avirulent pathogen (PubMed:23250427).
Localized in endosome-like vesicles displaying rapid cytosolic streaming (PubMed:20332379).
Accumulates in the nucleus following pathogen-associated molecular pattern (PAMP) treatment or infection with an avirulent pathogen (PubMed:23250427).
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Loss of ATPase activity. Increased sensitivity to turnip crinkle virus (TCV) leading to spreading hypersensitive response (HR) and impaired control of viral replication and spread. Suppression of HR-like cell death induced by Pseudomonas syringae avrRpt2. Suppression of lesion formation and partial suppression of stunted growth of ssi4 mutant (PubMed:18191794).
In the double mutant crt1-2 crh1-1, compromised resistance to avirulent Pseudomonas syringae and Hyaloperonospora arabidopsidis associated with compromised cytosolic calcium accumulation upon infection (PubMed:20332379).
Impaired gene silencing due to decondensation of chromocenters leading to the derepression of DNA-methylated genes and transposable elements (TEs); DNA and histone methylation seems normal (PubMed:22555433, PubMed:24799676).
The double mutant crt1-2 crh1-1 exhibits also an increased sensitivity to turnip crinkle virus (TCV), and reduced defense mediated by flg22 against Pseudomonas syringae (Pst). Impaired non-host resistance toward P. infestans and altered systemic acquired resistance (SAR) triggered by P. syringae pv. maculicola (Psm) AvrRpt2 cor-. Reduced sensitivity to the DNA-damaging agent mitomycin C (PubMed:23250427).
In the double mutant crt1-2 crh1-1, compromised resistance to avirulent Pseudomonas syringae and Hyaloperonospora arabidopsidis associated with compromised cytosolic calcium accumulation upon infection (PubMed:20332379).
Impaired gene silencing due to decondensation of chromocenters leading to the derepression of DNA-methylated genes and transposable elements (TEs); DNA and histone methylation seems normal (PubMed:22555433, PubMed:24799676).
The double mutant crt1-2 crh1-1 exhibits also an increased sensitivity to turnip crinkle virus (TCV), and reduced defense mediated by flg22 against Pseudomonas syringae (Pst). Impaired non-host resistance toward P. infestans and altered systemic acquired resistance (SAR) triggered by P. syringae pv. maculicola (Psm) AvrRpt2 cor-. Reduced sensitivity to the DNA-damaging agent mitomycin C (PubMed:23250427).
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 61 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000434976 | 1-635 | Protein MICRORCHIDIA 1 | |||
Sequence: MAKNYTVADVVNIDSDSDSDDDNGGVIGMVPSLASLIENQKVSIADAATVAPRETLECRSFWKAGENFVIPSSVTLTAIGMVEHARVHPKFLHSNATSHKWAFGAIAELLDNAVDEIQNGATVVKIDKINIVKDNTPALVFQDNGGGMDPNGIRKCMSLGYSSKKSNTTIGQYGNGFKTSTMRLGADAMVFSRSTRGGKSTQSIGLLSYTFLRKTGQDDVIVPMIDFDISSDSPQPIIYGSPGDWSTNLNILLKWSPFSTMVELLQQFEDIGTHGTKVIIYNLWLNDEGIYELSFDDDDVDIRLRDENAQDGKRLHAKTLEVRSHISYRYRHSLRAYISMLYLKKFKNFKIILRGVSVAQFNIADEFRHPETIMYKPQAAAVDYAATGIKVGFIKEAPKLPICGFNVYHKNRLIRPFWKVVLEGSTRGNGVMGVLEANFIEPAHDKQDFERSSLFLRLEARLKRITSDYWQNHCHIFGYQTAQIPADKSKRTVIPDQPPTVNTYNPSPLPSDRISHGGPIIREINLSNATSSRTAAVAAPHLRNYTGLRNNFQPVQLNPQPPAAGDTGNNLVGKLAAEIREENLQLFMRCEEYVKKENEVEQTVKSLEKELEEIKSKCAQLALLVDAKKKEMQQV |
Proteomic databases
Expression
Interaction
Subunit
Homodimer and heterodimer with MORC6. Component of an RNA-directed DNA methylation (RdDM) complex that contains at least MORC6, MORC1/CRT1, MORC2, SWI3D and SUVH9. Binds directly to SUVH2 and SUVH9 (PubMed:24465213, PubMed:24799676).
Interacts with the resistance proteins RCY1, RPM1, SNC1, RPP8, SSI4 and RPS2 (PubMed:18191794, PubMed:20332379).
The interactions with various resistance proteins are disrupted when these resistance proteins are activated (PubMed:20332379).
Interacts with the PAMP recognition receptor FLS2 (PubMed:23250427).
Interacts with the resistance proteins RCY1, RPM1, SNC1, RPP8, SSI4 and RPS2 (PubMed:18191794, PubMed:20332379).
The interactions with various resistance proteins are disrupted when these resistance proteins are activated (PubMed:20332379).
Interacts with the PAMP recognition receptor FLS2 (PubMed:23250427).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 491-511 | Disordered | ||||
Sequence: RTVIPDQPPTVNTYNPSPLPS | ||||||
Compositional bias | 495-510 | Polar residues | ||||
Sequence: PDQPPTVNTYNPSPLP | ||||||
Coiled coil | 588-635 | |||||
Sequence: MRCEEYVKKENEVEQTVKSLEKELEEIKSKCAQLALLVDAKKKEMQQV |
Sequence similarities
Belongs to the MORC ATPase protein family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length635
- Mass (Da)70,807
- Last updated2003-06-01 v1
- Checksum0889A04DBCFBAB8D
Sequence caution
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 77 | in Ref. 4; AAK26024 | ||||
Sequence: T → I | ||||||
Compositional bias | 495-510 | Polar residues | ||||
Sequence: PDQPPTVNTYNPSPLP |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z99708 EMBL· GenBank· DDBJ | CAB16854.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AL022141 EMBL· GenBank· DDBJ | CAA18135.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AL161589 EMBL· GenBank· DDBJ | CAB80300.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002687 EMBL· GenBank· DDBJ | AEE86644.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF360314 EMBL· GenBank· DDBJ | AAK26024.1 EMBL· GenBank· DDBJ | mRNA | ||
BT001940 EMBL· GenBank· DDBJ | AAN71939.1 EMBL· GenBank· DDBJ | mRNA | ||
AK222136 EMBL· GenBank· DDBJ | BAD95165.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |