Q84MD8 · FHYRK_ARATH

Function

function

Bifunctional enzyme that catalyzes the hydrolysis of flavin-mononucleotide (FMN) to riboflavin (vitamin B2) and the phosphorylation of riboflavin to form (FMN) coenzyme.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
1.03 μMriboflavin7.5
2 μMATP7.5
6.74 μMFMN7.5

pH Dependence

Optimum pH is acidic (5.5-6.0) for FMN phosphatase activity and basic for riboflavin kinase activity.

Temperature Dependence

Optimum temperature is 55 degrees Celsius.

Pathway

Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site17Nucleophile; for FMN phosphatase activity
Binding site17Mg2+ (UniProtKB | ChEBI)
Active site19Proton donor; for FMN phosphatase activity
Binding site19Mg2+ (UniProtKB | ChEBI)
Binding site248ATP (UniProtKB | ChEBI)
Binding site254ATP (UniProtKB | ChEBI)
Binding site260ATP (UniProtKB | ChEBI)
Binding site260Mg2+ (UniProtKB | ChEBI)
Binding site262ATP (UniProtKB | ChEBI)
Active site312Nucleophile; for riboflavin kinase activity
Binding site315ATP (UniProtKB | ChEBI)
Binding site317ATP (UniProtKB | ChEBI)
Binding site324ATP (UniProtKB | ChEBI)
Binding site337FMN (UniProtKB | ChEBI)
Binding site342FMN (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular FunctionFMN adenylyltransferase activity
Molecular FunctionFMN hydrolase activity
Molecular Functionmagnesium ion binding
Molecular Functionriboflavin kinase activity
Biological ProcessFMN biosynthetic process
Biological Processriboflavin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional riboflavin kinase/FMN phosphatase

Including 2 domains:

  • Recommended name
    FMN phosphatase
  • EC number
  • Alternative names
    • FMN phosphohydrolase
  • Recommended name
    Riboflavin kinase
  • EC number
  • Alternative names
    • Flavokinase

Gene names

    • Name
      FHY
    • Synonyms
      FMN
    • ORF names
      F18E5.90
    • Ordered locus names
      At4g21470

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q84MD8
  • Secondary accessions
    • O65412

Proteomes

Organism-specific databases

Genome annotation databases

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylserine
ChainPRO_00004290252-379Bifunctional riboflavin kinase/FMN phosphatase

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

In the N-terminal section; belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.
In the C-terminal section; belongs to the flavokinase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    379
  • Mass (Da)
    42,110
  • Last updated
    2003-06-01 v1
  • Checksum
    01C279F602C86E3F
MSMSNSLKKLSSCVLIDLDGTLINTDGVVGDILRKYLCKYGKQWDGRESLKIVGKTPVEAATTIVEDYELPCKVDEFNSEFYPLFSAQMDKIKSLPGANRLIRHLKCHGVPVALASNSSRANIESKISYHEGWKECFSVIVGSDEVSKGKPSPDIFLEAAKRLKKDPADCLVIEDSVPGVMAGKAAGTKVIAVPSLPKQTHLYTSADEVINSLLDIRLEKWGLPPFQDWIENTLPIDPWHIGGPVIKGFGRGSKVLGIPTANLSTKDYADELVEHPSGVYFGWAGLAKRGVFKMVMSIGWNPYFNNKEKTIEPWLLHDFTEDFYGEELRLIIVGYIRPEANFSSLESLIAKIHEDREVAEKALDLPSYAKFKGDPYLTK

Sequence caution

The sequence CAA18711.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence CAB81254.1 differs from that shown. Reason: Erroneous gene model prediction

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY878327
EMBL· GenBank· DDBJ
AAX98488.1
EMBL· GenBank· DDBJ
mRNA
AL022603
EMBL· GenBank· DDBJ
CAA18711.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AL161555
EMBL· GenBank· DDBJ
CAB81254.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002687
EMBL· GenBank· DDBJ
AEE84457.1
EMBL· GenBank· DDBJ
Genomic DNA
BT006373
EMBL· GenBank· DDBJ
AAP21181.1
EMBL· GenBank· DDBJ
mRNA
AK227237
EMBL· GenBank· DDBJ
BAE99274.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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