Q83VN1 · Q83VN1_STRSU

  • Protein
    DNA gyrase subunit A
  • Gene
    gyrA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.

Catalytic activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA.
    EC:5.6.2.2 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

1814100200300400500600700800
TypeIDPosition(s)Description
Active site120O-(5'-phospho-DNA)-tyrosine intermediate

GO annotations

AspectTerm
Cellular Componentchromosome
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular FunctionDNA negative supercoiling activity
Biological ProcessDNA topological change
Biological ProcessDNA-templated DNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA gyrase subunit A
  • EC number

Gene names

    • Name
      gyrA

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • 88-1861
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Lactobacillales > Streptococcaceae > Streptococcus

Accessions

  • Primary accession
    Q83VN1

Subcellular Location

Keywords

Interaction

Subunit

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain9-462DNA topoisomerase type IIA
Motif524-530GyrA-box

Sequence similarities

Belongs to the type II topoisomerase GyrA/ParC subunit family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    814
  • Mass (Da)
    91,366
  • Last updated
    2003-06-01 v1
  • Checksum
    8E23796D7E65D7F5
MQDKNLVTVNLTNEMKTSFIDYAMSVIVARALPDVRDGLKPVHRRILYGMNELGITPDKPHKKSARITGDVMGKYHPHGDSAIYEAMVRMAQWWSYRHMLVDGHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVDFADNYDSSEREPEVLPARFPNLLVNGTTGIAVGMATNIPPHNLGETIDAVKLVMDNPDVTTREIMEVLPGPDFPTGALVMGKSGIHRAYETGKGSIVLRSRTEIEEYGNGRERIVVTEFPYMVNKSKVQEHIVKLVQEKRIEGITAVRDESNREGVRFVIEVRRDASANVILNNLFKQTQMQTNFSFNMLAIQNGVPKILSVRQILESYIEHQKEVVTRRTQFDKEKAEARAHILEGLLIALDHIDEVIRIIRNSETDALAQAELMERFDLSERQSQAILDMRLRRLTGLERDKIQSEYDELVALIADLTDILAKPERVVLIIKEELEEVKRKYADPRRTELMVGEVLSLEDEDLIEEADVLITLSNQGYIKRLAQDEFQAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRLKGYEIPEYGRTAKGLPVVNLLKLEENESIQTIINVTKDQTAEHYLFFATRQGIVKRANVNEFSNIRQNGLKALNLKDDDELINVFLTDGQADVIMGTKFGYSVRFAETDVRNMGRTATGVRGIKLREGDHLVGATIVSDDQEVLVLSENGYGKRTPASEYPTKGRGGKGIITLKVADKNGPLAGLTTVQGDEDIMVITDTGVIIRTSIANISQTGRSTMGVKVMRLDEEAKIMTFALVDAAEEKEDEE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB081334
EMBL· GenBank· DDBJ
BAC56225.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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