Q83RJ4 · IPA3_SHIFL

  • Protein
    E3 ubiquitin-protein ligase ipaH3
  • Gene
    ipaH3
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin ligase that interferes with host's ubiquitination pathway. Synthesizes a 'Lys-48'-linked ubiquitin chain, which requires non-covalent binding between ubiquitin and the host ubiquitin-conjugating enzyme UBE2D1.

Catalytic activity

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

157150100150200250300350400450500550
TypeIDPosition(s)Description
Active site363Glycyl thioester intermediate

GO annotations

AspectTerm
Cellular Componentextracellular region
Cellular Componenthost cell cytoplasm
Molecular Functionubiquitin-protein transferase activity
Biological Processmodulation of process of another organism
Biological Processprotein ubiquitination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    E3 ubiquitin-protein ligase ipaH3
  • EC number
  • Alternative names
    • RING-type E3 ubiquitin transferase ipaH3

Gene names

    • Name
      ipaH3
    • Ordered locus names
      SF1383

Organism names

  • Taxonomic identifier
  • Strain
    • 301 / Serotype 2a
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Shigella

Accessions

  • Primary accession
    Q83RJ4

Proteomes

Subcellular Location

Secreted
Host cytoplasm
Note: Secreted via Mxi-Spa type III secretion system (T3SS), and delivered into the host cytoplasm.

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis363Loss of ubiquitin ligase activity.
Mutagenesis365Loss of ubiquitin ligase activity, but acts as a thioesterase that uses Cys-363 to hydrolyze the ubiquitin-E2 thioester.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003917541-571E3 ubiquitin-protein ligase ipaH3

Post-translational modification

Ubiquitinated in the presence of host E1 ubiquitin-activating enzyme, E2 ubiquitin-conjugating enzyme UBE2D3 and ubiquitin.

Keywords

Proteomic databases

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, repeat, domain.

TypeIDPosition(s)Description
Region1-260Interaction with target proteins
Repeat58-81LRR 1
Repeat83-99LRR 2
Repeat100-119LRR 3
Repeat120-144LRR 4
Repeat146-159LRR 5
Repeat160-184LRR 6
Repeat186-202LRR 7
Repeat205-229LRR 8
Repeat232-260LRR 9
Region269-278Linker
Region279-571E3 ubiquitin-protein ligase catalytic domain
Domain287-497NEL

Domain

The LRR (leucine-rich repeat) domain forms a slightly curved solenoid and may mediate interaction with target proteins.

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    571
  • Mass (Da)
    64,943
  • Last updated
    2003-06-01 v1
  • Checksum
    B0DF655E1540D77D
MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGENRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASLEYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPTSLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENRITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTAQPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSTSAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWAQAEEQKYEMLENEYSQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQVTDEVLALRLSENGSQLHHS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE005674
EMBL· GenBank· DDBJ
AAN42986.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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