Q833J1 · TCPF_ENTFA

Function

function

Virulence factor that suppresses host Toll-like receptor (TLR)-mediated cytokine production upon infection (PubMed:25147569, PubMed:25369374).
Acts as a NAD+ hydrolase (NADase) by catalyzing cleavage of NAD+ into ADP-D-ribose (ADPR) and nicotinamide (PubMed:29395922).
Interacts with various phosphatidylinositol phosphates, such as phosphatidylinositol 3-phosphate (PtdIns3P), phosphatidylinositol 4-phosphate (PtdIns4P), phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P), phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P), phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P), and phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P) (PubMed:25147569).

Catalytic activity

Features

Showing features for binding site, active site.

127420406080100120140160180200220240260
TypeIDPosition(s)Description
Binding site12-13NAD+ (UniProtKB | ChEBI)
Binding site47NAD+ (UniProtKB | ChEBI)
Active site83

GO annotations

AspectTerm
Molecular FunctionNAD+ nucleosidase activity
Molecular FunctionNAD+ nucleotidase, cyclic ADP-ribose generating
Molecular Functionphosphatidylinositol-3,4,5-trisphosphate binding
Molecular Functionphosphatidylinositol-3,4-bisphosphate binding
Molecular Functionphosphatidylinositol-3,5-bisphosphate binding
Molecular Functionphosphatidylinositol-3-phosphate binding
Molecular Functionphosphatidylinositol-4,5-bisphosphate binding
Molecular Functionphosphatidylinositol-4-phosphate binding
Biological ProcessNAD catabolic process
Biological Processnegative regulation of MyD88-dependent toll-like receptor signaling pathway
Biological Processsignal transduction

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NAD(+) hydrolase TcpF
  • EC number
  • Alternative names
    • TIR domain-containing protein in E.faecalis
      (tcpF
      )

Gene names

    • Name
      tcpF
    • Ordered locus names
      EF_1959

Organism names

Accessions

  • Primary accession
    Q833J1

Proteomes

Phenotypes & Variants

Miscellaneous

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004491441-274NAD+ hydrolase TcpF

Interaction

Subunit

Interacts with host MYD88.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain3-140TIR

Domain

The TIR domain mediates NAD+ hydrolase (NADase) activity. Self-association of TIR domains is required for NADase activity.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    274
  • Mass (Da)
    31,799
  • Last updated
    2003-06-01 v1
  • Checksum
    D10115B1C15B1A7B
MSNGKKIFISHSSKDQEYVDAFIQLLKKFGFRTQDIFYSSTIETGVQPGELIFDTIKRELTNQPVMLYFLSDHYYQSIPCLNEMGASWMLSDKHYPIALNNFSMKDMKGVISSERLAIAFNDKTSTNEINCLLKKLSHDTDVQAEPDFELNVEKNIQPFQNKLTQLIRQASYLKPDEKGYFETILSTHRPVYGTAKGVYDCFKLPSLIEPKSLGLDTLSEDESHWLFFFLTWGTFQEGEKVRFKLKKDKAYNNREFSDIGKCKNIYVSYLEKVE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE016830
EMBL· GenBank· DDBJ
AAO81706.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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