Q82IE8 · ISPH_STRAW

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site21[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site50(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site50dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site50isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site83(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site83dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site83isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site105[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site133(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site133dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site133isopentenyl diphosphate (UniProtKB | ChEBI)
Active site135Proton donor
Binding site173(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site203[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site231(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site231dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site231isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site232(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site232dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site232isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site233(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site233dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site233isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site276(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site276dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site276isopentenyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • Ordered locus names
      SAV_3210

Organism names

Accessions

  • Primary accession
    Q82IE8

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001288741-3384-hydroxy-3-methylbut-2-enyl diphosphate reductase

Structure

Family & Domains

Sequence similarities

Belongs to the IspH family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    338
  • Mass (Da)
    36,591
  • Last updated
    2003-06-01 v1
  • Checksum
    808272480F31C313
MVSMTASSGRRVLLAAPRGYCAGVDRAVIAVEKALEQYGAPIYVRHEIVHNKYVVQTLERKGAIFVERTAEVPEGAIVMFSAHGVAPVVHEEAARGKLATIDATCPLVTKVHKEAVRFANEDFDILLIGHEGHEEVIGTSGEAPEHITLVDGPGDVAKVEVRDPSKVVWLSQTTLSVDETMETVDALKEKFPQLISPPSDDICYATQNRQLAVKQMGEEADLVIVVGSRNSSNSVRLVEVAKLAGARDAYLVDFADEIDEAWLEGVSTVGVTSGASVPEILVEQVLEWLSQRGFEDVEIVKAAEESITFSLPKELRRDLRAEAAALVEQRTGNGPSAE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BA000030
EMBL· GenBank· DDBJ
BAC70921.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp