Q829W3 · G3P2_STRAW

Function

function

Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.

Catalytic activity

Activity regulation

Inhibited by pentalenolactone.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.33 mMD-glyceraldehyde 3-phosphate
kcat is 165 sec-1 with D-glyceraldehyde 3-phosphate.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site12-13NAD+ (UniProtKB | ChEBI)
Binding site35NAD+ (UniProtKB | ChEBI)
Binding site79NAD+ (UniProtKB | ChEBI)
Binding site121NAD+ (UniProtKB | ChEBI)
Binding site152-154D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Active site153Nucleophile
Site180Activates thiol group during catalysis
Binding site183D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site184NAD+ (UniProtKB | ChEBI)
Binding site198D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site211-212D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site234D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site316NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionglyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Molecular FunctionNAD binding
Molecular FunctionNADP binding
Biological Processglucose metabolic process
Biological Processglycolytic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glyceraldehyde-3-phosphate dehydrogenase 2
  • EC number
  • Short names
    GAPDH 2
  • Alternative names
    • NAD-dependent glyceraldehyde-3-phosphate dehydrogenase
    • PL-sensitive glyceraldehyde-3-phosphate dehydrogenase

Gene names

    • Name
      gap2
    • Ordered locus names
      SAV_6296

Organism names

Accessions

  • Primary accession
    Q829W3

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004220151-335Glyceraldehyde-3-phosphate dehydrogenase 2

Interaction

Subunit

Homotetramer.

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    335
  • Mass (Da)
    36,162
  • Last updated
    2003-06-01 v1
  • Checksum
    8A006B5A173776B7
MTIRVGINGFGRIGRNYFRALLEQGADIEIVAVNDLGDTATTAHLLKYDTILGRLKAEVTHTADTITVDGKTIKVFSERNPADIPWGELNVDIVIESTGIFTKKADAEKHIAGGAKKVLISAPASDEDITIVLGVNEDKYDPAKHNVISNASCTTNCVAPMAKVLDENFGIVKGLMTTIHAYTNDQRILDFPHKDLRRARAAAENIIPTTTGAAKATALVLPQLKGKMDGISMRVPVPTGSATDLVVEVSREVTKDEVNAAFKKAAEGELQGYLSYTEDPIVSSDIVGDPSSCTFDSAMTMVMEGTSVKILGWYDNEWGYSNRLVDLTVFVGNQL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BA000030
EMBL· GenBank· DDBJ
BAC74007.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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