Q81WK7 · CODY_BACAN

  • Protein
    Global transcriptional regulator CodY
  • Gene
    codY
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

DNA-binding global transcriptional regulator which is involved in the adaptive response to starvation and acts by directly or indirectly controlling the expression of numerous genes in response to nutrient availability. During rapid exponential growth, CodY is highly active and represses genes whose products allow adaptation to nutrient depletion.
In B.anthracis, CodY represses or activates directly or indirectly several hundred genes, including genes involved in amino acid biosynthesis and transport, as well as nucleic acid metabolism, the pentose pathway, the tricarboxylic acid cycle, acetyl coenzyme A synthesis and oxidative stress functions (PubMed:19651859, PubMed:23292769).
Among others, represses directly the expression of sap and eag, encoding the two surface layer (S-layer) proteins, by binding directly to their promoter region (PubMed:23292769).
Binds to a 11-bp non-palindromic consensus DNA motif, the CodY-binding site (PubMed:23292769).
Additionally, in pathogenic bacteria, CodY also regulates virulence gene expression and provides a regulatory link between metabolism and pathogenesis (PubMed:19651859, PubMed:21911592).
Is essential for the acquisition of iron from the host during infection (PubMed:21911592).
Interacts with the atxA global regulator promoter region, but it has only a small effect on atxA transcription, specifically during the exponential growth phase (PubMed:19651859, PubMed:23292769).
May regulate toxin gene expression by post-translationally regulating the accumulation of AtxA (PubMed:19651859).
Has no discernible effect on the regulation of capsule biosynthesis (PubMed:21911592).
Binds to GTP and exhibits weak GTPase activity (PubMed:28472295).

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.16 mMGTP

Features

Showing features for dna binding.

TypeIDPosition(s)Description
DNA binding203-222H-T-H motif

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionDNA binding
Molecular FunctionDNA-binding transcription factor activity
Molecular FunctionGTP binding
Biological Processnegative regulation of DNA-templated transcription

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Global transcriptional regulator CodY

Gene names

    • Name
      codY
    • Ordered locus names
      BA_3966, GBAA_3966, BAS3679

Organism names

  • Taxonomic identifier
  • Strains
    • Ames / isolate Porton
    • Ames ancestor
    • Sterne
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus > Bacillus cereus group

Accessions

  • Primary accession
    Q81WK7
  • Secondary accessions
    • Q6HUQ8
    • Q6KNY9

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Disruption of the gene results in strongly attenuated virulence in both mouse and guinea pig models of infection (PubMed:19651859, PubMed:21911592).
Disruption leads to a delayed initiation of dissemination but similar kinetics of subsequent spread of the bacilli (PubMed:21911592).
The mutant cannot grow on heme iron as sole iron source (PubMed:21911592).
Disruption alters the expression of more than 200 genes, including the toxin genes (PubMed:19651859).
It also causes a decrease in AtxA protein accumulation but it does not affect its transcription or its translation (PubMed:19651859).
It does not affect either ex vivo or in vivo capsulation (PubMed:21911592).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis215Abolishes autophosphorylation.

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00002132131-259Global transcriptional regulator CodY
Modified residue215Phosphoserine

Post-translational modification

Autophosphorylated on Ser-215 (PubMed:28472295).
Autophosphorylation may regulate CodY activity (PubMed:28472295).

Keywords

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Monomer and homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-155GAF domain

Sequence similarities

Belongs to the CodY family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    259
  • Mass (Da)
    28,774
  • Last updated
    2003-06-01 v1
  • Checksum
    91C04A2CFA20EFD2
MELLAKTRKLNALLQSAAGKPVNFREMSDTMCEVIEANVFVVSRRGKLLGYAIHQQIENERMKQMLAERQFPEEYTQSLFNITETSSNLDVNSAYTAFPVENKELFGQGLTTIVPIVGGGERLGTLVLARLGQEFLDDDLILAEYSSTVVGMEILREKAEEIEEEARSKAVVQMAISSLSYSELEAIEHIFEELNGTEGLLVASKIADRVGITRSVIVNALRKLESAGVIESRSLGMKGTYIKVLNDKFLHELAKLKTN

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE016879
EMBL· GenBank· DDBJ
AAP27695.1
EMBL· GenBank· DDBJ
Genomic DNA
AE017334
EMBL· GenBank· DDBJ
AAT33080.1
EMBL· GenBank· DDBJ
Genomic DNA
AE017225
EMBL· GenBank· DDBJ
AAT55981.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp