Q81WK7 · CODY_BACAN
- ProteinGlobal transcriptional regulator CodY
- GenecodY
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids259 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
DNA-binding global transcriptional regulator which is involved in the adaptive response to starvation and acts by directly or indirectly controlling the expression of numerous genes in response to nutrient availability. During rapid exponential growth, CodY is highly active and represses genes whose products allow adaptation to nutrient depletion.
In B.anthracis, CodY represses or activates directly or indirectly several hundred genes, including genes involved in amino acid biosynthesis and transport, as well as nucleic acid metabolism, the pentose pathway, the tricarboxylic acid cycle, acetyl coenzyme A synthesis and oxidative stress functions (PubMed:19651859, PubMed:23292769).
Among others, represses directly the expression of sap and eag, encoding the two surface layer (S-layer) proteins, by binding directly to their promoter region (PubMed:23292769).
Binds to a 11-bp non-palindromic consensus DNA motif, the CodY-binding site (PubMed:23292769).
Additionally, in pathogenic bacteria, CodY also regulates virulence gene expression and provides a regulatory link between metabolism and pathogenesis (PubMed:19651859, PubMed:21911592).
Is essential for the acquisition of iron from the host during infection (PubMed:21911592).
Interacts with the atxA global regulator promoter region, but it has only a small effect on atxA transcription, specifically during the exponential growth phase (PubMed:19651859, PubMed:23292769).
May regulate toxin gene expression by post-translationally regulating the accumulation of AtxA (PubMed:19651859).
Has no discernible effect on the regulation of capsule biosynthesis (PubMed:21911592).
Binds to GTP and exhibits weak GTPase activity (PubMed:28472295).
Among others, represses directly the expression of sap and eag, encoding the two surface layer (S-layer) proteins, by binding directly to their promoter region (PubMed:23292769).
Binds to a 11-bp non-palindromic consensus DNA motif, the CodY-binding site (PubMed:23292769).
Additionally, in pathogenic bacteria, CodY also regulates virulence gene expression and provides a regulatory link between metabolism and pathogenesis (PubMed:19651859, PubMed:21911592).
Is essential for the acquisition of iron from the host during infection (PubMed:21911592).
Interacts with the atxA global regulator promoter region, but it has only a small effect on atxA transcription, specifically during the exponential growth phase (PubMed:19651859, PubMed:23292769).
May regulate toxin gene expression by post-translationally regulating the accumulation of AtxA (PubMed:19651859).
Has no discernible effect on the regulation of capsule biosynthesis (PubMed:21911592).
Binds to GTP and exhibits weak GTPase activity (PubMed:28472295).
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.16 mM | GTP |
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 203-222 | H-T-H motif | ||||
Sequence: ASKIADRVGITRSVIVNALR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | DNA binding | |
Molecular Function | DNA-binding transcription factor activity | |
Molecular Function | GTP binding | |
Biological Process | negative regulation of DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameGlobal transcriptional regulator CodY
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus > Bacillus cereus group
Accessions
- Primary accessionQ81WK7
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Disruption of the gene results in strongly attenuated virulence in both mouse and guinea pig models of infection (PubMed:19651859, PubMed:21911592).
Disruption leads to a delayed initiation of dissemination but similar kinetics of subsequent spread of the bacilli (PubMed:21911592).
The mutant cannot grow on heme iron as sole iron source (PubMed:21911592).
Disruption alters the expression of more than 200 genes, including the toxin genes (PubMed:19651859).
It also causes a decrease in AtxA protein accumulation but it does not affect its transcription or its translation (PubMed:19651859).
It does not affect either ex vivo or in vivo capsulation (PubMed:21911592).
Disruption leads to a delayed initiation of dissemination but similar kinetics of subsequent spread of the bacilli (PubMed:21911592).
The mutant cannot grow on heme iron as sole iron source (PubMed:21911592).
Disruption alters the expression of more than 200 genes, including the toxin genes (PubMed:19651859).
It also causes a decrease in AtxA protein accumulation but it does not affect its transcription or its translation (PubMed:19651859).
It does not affect either ex vivo or in vivo capsulation (PubMed:21911592).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 215 | Abolishes autophosphorylation. | ||||
Sequence: S → A |
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000213213 | 1-259 | Global transcriptional regulator CodY | |||
Sequence: MELLAKTRKLNALLQSAAGKPVNFREMSDTMCEVIEANVFVVSRRGKLLGYAIHQQIENERMKQMLAERQFPEEYTQSLFNITETSSNLDVNSAYTAFPVENKELFGQGLTTIVPIVGGGERLGTLVLARLGQEFLDDDLILAEYSSTVVGMEILREKAEEIEEEARSKAVVQMAISSLSYSELEAIEHIFEELNGTEGLLVASKIADRVGITRSVIVNALRKLESAGVIESRSLGMKGTYIKVLNDKFLHELAKLKTN | ||||||
Modified residue | 215 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Autophosphorylated on Ser-215 (PubMed:28472295).
Autophosphorylation may regulate CodY activity (PubMed:28472295).
Autophosphorylation may regulate CodY activity (PubMed:28472295).
Keywords
- PTM
PTM databases
Expression
Gene expression databases
Interaction
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-155 | GAF domain | ||||
Sequence: MELLAKTRKLNALLQSAAGKPVNFREMSDTMCEVIEANVFVVSRRGKLLGYAIHQQIENERMKQMLAERQFPEEYTQSLFNITETSSNLDVNSAYTAFPVENKELFGQGLTTIVPIVGGGERLGTLVLARLGQEFLDDDLILAEYSSTVVGMEIL |
Sequence similarities
Belongs to the CodY family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length259
- Mass (Da)28,774
- Last updated2003-06-01 v1
- Checksum91C04A2CFA20EFD2
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE016879 EMBL· GenBank· DDBJ | AAP27695.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE017334 EMBL· GenBank· DDBJ | AAT33080.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE017225 EMBL· GenBank· DDBJ | AAT55981.1 EMBL· GenBank· DDBJ | Genomic DNA |