Q81F27 · ILVC2_BACCR

Function

function

Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 magnesium ions per subunit.

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.
Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site24-27NADP+ (UniProtKB | ChEBI)
Binding site47NADP+ (UniProtKB | ChEBI)
Binding site51NADP+ (UniProtKB | ChEBI)
Binding site81-84NADP+ (UniProtKB | ChEBI)
Active site106
Binding site132NADP+ (UniProtKB | ChEBI)
Binding site189Mg2+ 1 (UniProtKB | ChEBI)
Binding site189Mg2+ 2 (UniProtKB | ChEBI)
Binding site193Mg2+ 1 (UniProtKB | ChEBI)
Binding site225Mg2+ 2 (UniProtKB | ChEBI)
Binding site229Mg2+ 2 (UniProtKB | ChEBI)
Binding site250substrate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionketol-acid reductoisomerase activity
Molecular Functionmagnesium ion binding
Molecular FunctionNADP binding
Biological Processisoleucine biosynthetic process
Biological Processvaline biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ketol-acid reductoisomerase (NADP(+)) 2
  • EC number
  • Short names
    KARI 2
  • Alternative names
    • Acetohydroxy-acid isomeroreductase 2
      (AHIR 2
      )
    • Alpha-keto-beta-hydroxylacyl reductoisomerase 2
    • Ketol-acid reductoisomerase type 1
    • Ketol-acid reductoisomerase type I

Gene names

    • Name
      ilvC2
    • Ordered locus names
      BC_1779

Organism names

Accessions

  • Primary accession
    Q81F27

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001512751-335Ketol-acid reductoisomerase (NADP+) 2

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-180KARI N-terminal Rossmann
Domain181-326KARI C-terminal knotted

Sequence similarities

Belongs to the ketol-acid reductoisomerase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    335
  • Mass (Da)
    36,921
  • Last updated
    2003-06-01 v1
  • Checksum
    07741D1B931B3E9F
MKTYYEKDANVELLQGKTVAVVGYGSQGHAQAQNLRDSGVEVVVGVRPGKSYEVAKADGFEVMSVSEAVRTAQVVQMLLPDEQQAHVYKAEVEENLREGQMLLFSHGFNIHFGQINPPSYVDVAMVAPKSPGHLVRRVFQEGNGVPALVAVHQDATGTALHVALAYAKGVGCTRAGVIETTFQEETETDLFGEQAVLCGGVTALVKAGFETLTEGGYRPEIAYFECLHELKLIVDLMYEGGLTTMRHSISDTAEFGDYVTGSRIVTDETKKEMKRVLTEIQQGEFAKKWILENQAGRPTYNAMKKAEQNHQLEKVGAELREMMSWIHAPKELVKK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE016877
EMBL· GenBank· DDBJ
AAP08753.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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