Q81EP4 · LDH1_BACCR
- ProteinL-lactate dehydrogenase 1
- Geneldh1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids314 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Catalyzes the conversion of lactate to pyruvate.
Catalytic activity
- (S)-lactate + NAD+ = H+ + NADH + pyruvate
Activity regulation
Allosterically activated by fructose 1,6-bisphosphate (FBP).
Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 16 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Binding site | 37 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 42 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 68 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 82-83 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: GL | ||||||
Binding site | 85 | substrate | ||||
Sequence: Q | ||||||
Binding site | 91 | substrate | ||||
Sequence: R | ||||||
Binding site | 121-123 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: ATN | ||||||
Binding site | 123-126 | substrate | ||||
Sequence: NPVD | ||||||
Binding site | 146 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 151-154 | substrate | ||||
Sequence: DSAR | ||||||
Binding site | 156 | beta-D-fructose 1,6-bisphosphate (UniProtKB | ChEBI); allosteric activator | ||||
Sequence: R | ||||||
Binding site | 171 | beta-D-fructose 1,6-bisphosphate (UniProtKB | ChEBI); allosteric activator | ||||
Sequence: H | ||||||
Active site | 178 | Proton acceptor | ||||
Sequence: H | ||||||
Binding site | 232 | substrate | ||||
Sequence: T |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | L-lactate dehydrogenase activity | |
Biological Process | glycolytic process | |
Biological Process | lactate metabolic process | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameL-lactate dehydrogenase 1
- EC number
- Short namesL-LDH 1
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus > Bacillus cereus group
Accessions
- Primary accessionQ81EP4
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000168321 | 1-314 | L-lactate dehydrogenase 1 | |||
Sequence: MKKGINRVVLVGTGAVGCSYAYCMINQAVAEEFVLVDVNEAKAEGEAMDLSHAVPFAPAPTRVWKGSYEDCKDADLVVITAGLPQKPGETRLDLVEKNAKIFKQIVRSIMDSGFDGIFLIATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLGEYFDIGPHNIHAYIIGEHGDTELPVWSHVSVGIQKLQTLLEKDNTYNQEDLDKIFINVRDAAYHIIERKGATYYGIGMSLLRVTKAILNDENSVLTVSAYLEGQYGQKDVYIGVPAVLNRGGVREILEVELSEDEELKFDHSVQVLKETMAPVL | ||||||
Modified residue | 223 | Phosphotyrosine | ||||
Sequence: Y |
Keywords
- PTM
Interaction
Structure
Sequence
- Sequence statusComplete
- Length314
- Mass (Da)34,789
- Last updated2003-06-01 v1
- Checksum19DC3B1C2083AB2B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE016877 EMBL· GenBank· DDBJ | AAP08895.1 EMBL· GenBank· DDBJ | Genomic DNA |