Q80YV4 · PANK4_MOUSE

  • Protein
    4'-phosphopantetheine phosphatase
  • Gene
    Pank4
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Phosphatase which shows a preference for 4'-phosphopantetheine and its oxidatively damaged forms (sulfonate or S-sulfonate), providing strong indirect evidence that the phosphatase activity pre-empts damage in the coenzyme A (CoA) pathway. Hydrolyzing excess 4'-phosphopantetheine could constitute a directed overflow mechanism to prevent its oxidation to the S-sulfonate, sulfonate, or other forms. Hydrolyzing 4'-phosphopantetheine sulfonate or S-sulfonate would forestall their conversion to inactive forms of CoA and acyl carrier protein. May play a role in the physiological regulation of CoA intracellular levels.

Caution

Despite belonging to the type II pantothenate kinase family, the pantothenate kinase domain contains a Val residue at position 147 and a Trp residue at position 211 instead of the two conserved active site residues, Glu and Arg. Lacks pantothenate kinase activity.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Ni2+ (UniProtKB | Rhea| CHEBI:49786 )

Activity regulation

Activity is strongly promoted by Co2+, Ni2+, Mg2+ and Mn2+. Activity is inhibited by EDTA.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site196acetyl-CoA (UniProtKB | ChEBI)
Binding site199acetyl-CoA (UniProtKB | ChEBI)
Binding site623Mn2+ (UniProtKB | ChEBI); catalytic; for phosphatase activity
Binding site624Mn2+ (UniProtKB | ChEBI); catalytic; for phosphatase activity
Binding site659Mn2+ (UniProtKB | ChEBI); catalytic; for phosphatase activity

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentextracellular space
Cellular Componentnucleus
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular Functionpantothenate kinase activity
Molecular Functionphosphatase activity
Biological Processcoenzyme A biosynthetic process
Biological Processcoenzyme A catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4'-phosphopantetheine phosphatase
  • EC number
  • Alternative names
    • Inactive pantothenic acid kinase 4
      (mPanK4
      )

Gene names

    • Name
      Pank4

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q80YV4
  • Secondary accessions
    • B1AZN5
    • B1AZN6
    • Q7M751
    • Q8BQE9

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Knockout mice have a body weight and a lifespan similar to wild-type animals. About 10% of the animals start developing cataract at 2 months. At 15 months, 50% homozygous animals are affected. Heterozygous animals develop cataract later, with 10% animals affected at 9 months and 25% at 15.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 36 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylalanine
ChainPRO_00002492482-8204'-phosphopantetheine phosphatase
Modified residue3203'-nitrotyrosine
Modified residue393Phosphoserine
Modified residue404Phosphoserine
Modified residue406Phosphothreonine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in liver, kidney, brain cortex and eye lens (at protein level).

Gene expression databases

Interaction

Subunit

Homodimer. Interacts with PKM.

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, motif.

TypeIDPosition(s)Description
Region1-20Disordered
Region2-402Pantothenate kinase
Region403-8204'-phosphopantetheine phosphatase
Motif771-775Subfamily II EGMGR motif

Domain

Subfamily II proteins have an EGMGR motif about 50 residues from the C-terminus (By similarity).
This motif lies near the metal-binding residues in the putative substrate-binding cleft 2 (By similarity).
Subfamily II proteins occur only in eukaryotes, in two forms: as a stand-alone unit in plants, and as a C-terminal domain of pantothenate kinases in plants, animals, and chytrid fungi (By similarity).

Sequence similarities

In the N-terminal section; belongs to the type II pantothenate kinase family.
In the C-terminal section; belongs to the damage-control phosphatase family. Phosphopantetheine phosphatase II subfamily.

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete

This entry describes 3 isoforms produced by Alternative splicing.

Q80YV4-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    820
  • Mass (Da)
    91,522
  • Last updated
    2006-09-05 v2
  • Checksum
    0D88CA48A8B87D3A
MAERGASGGGSGGDSLDKSITLPPDEIFRNLENAKRFAIDIGGSLTKLAYYSTVQHKVAKVRSFDHPGKDVEQDHEPPYEISVQEEITARLHFIKFENTYMEACLDFIRDHLVNTETKVIQATGGGAYKFKDLIEEKLRLKVDKEDVMTCLIKGCNFVLKNIPHEAFMYQKDSDPEFRFQTNHPNIFPYLLVNIGSGVSIVKVETEDRFEWIGGSSIGGGTFWGLGALLTKTKKFDELLQLASRGRHANVDMLVQDIYGGAHQTLGLSGNLIASSFGKSATADREFSKEDMAKSLLHMISNDIGQLACLYAKLHGLDRVYFGGFFIRGHPVTMRTITYSINFFSKGEVQALFLRHEGYLGAIGAFLKGAEQDNPNQYSWGENYAASSGLMSTSPELCPTQRARSGTFDLLEMDRLERPLVNLPLLLDPSSYVPDTVDLTDDALARQYWLTCFEEALDGVVKRAVASQPESMDAVERAEKFRQKYWGKLQTLRHQPFAYGTLTVRSLLDTREHCLNEFNFPDPYSKVKQKENGLALKCFQSVTRSLDSLGWEERQLALVKGLLAGNVFDWGAKAVSDVLESDPQFGFEEAKRKLQERPWLVDSYTKWLQRLKGPPHKCALIFADNSGIDIILGVFPFVRELLFRGTEVILACNSGPALNDVTYSESLIVAERIAAMDPIICTALREDRLLLVQTGSSSPCLDLSLCTSRTTTCMVLPFAMWVLWTKLKSLVEKCLSPLSILLACSVLSAKSRLDKGLAVLVRERGADLVVIEGMGRAIHTNYHALLRCESLKLAVVKNAWLAERLGGQLFSVIFKYEVPTK

Q80YV4-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Q80YV4-3

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
F7B6K4F7B6K4_MOUSEPank4473

Features

Showing features for sequence conflict, alternative sequence.

TypeIDPosition(s)Description
Sequence conflict81in Ref. 3; AAH50089
Sequence conflict146in Ref. 3; AAH50089
Sequence conflict173in Ref. 4; DAA00010
Sequence conflict205in Ref. 3; AAH50089
Sequence conflict243in Ref. 3; AAH50089
Sequence conflict351in Ref. 3; AAH50089
Alternative sequenceVSP_020379373-374in isoform 3
Alternative sequenceVSP_020380375-820in isoform 3
Sequence conflict479in Ref. 1; BAC34450
Sequence conflict635in Ref. 1; BAC34450
Alternative sequenceVSP_020381703-749in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK050894
EMBL· GenBank· DDBJ
BAC34450.1
EMBL· GenBank· DDBJ
mRNA
BX004788
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC050089
EMBL· GenBank· DDBJ
AAH50089.1
EMBL· GenBank· DDBJ
mRNA
BK000016
EMBL· GenBank· DDBJ
DAA00010.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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