Q80YD1 · SUV3_MOUSE
- ProteinATP-dependent RNA helicase SUPV3L1, mitochondrial
- GeneSupv3l1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids779 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Major helicase player in mitochondrial RNA metabolism. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (By similarity).
ATPase and ATP-dependent multisubstrate helicase, able to unwind double-stranded (ds) DNA and RNA, and RNA/DNA heteroduplexes in the 5'-to-3' direction. Plays a role in the RNA surveillance system in mitochondria; regulates the stability of mature mRNAs, the removal of aberrantly formed mRNAs and the rapid degradation of non coding processing intermediates. Also implicated in recombination and chromatin maintenance pathways. May protect cells from apoptosis. Associates with mitochondrial DNA
ATPase and ATP-dependent multisubstrate helicase, able to unwind double-stranded (ds) DNA and RNA, and RNA/DNA heteroduplexes in the 5'-to-3' direction. Plays a role in the RNA surveillance system in mitochondria; regulates the stability of mature mRNAs, the removal of aberrantly formed mRNAs and the rapid degradation of non coding processing intermediates. Also implicated in recombination and chromatin maintenance pathways. May protect cells from apoptosis. Associates with mitochondrial DNA
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Activity regulation
Helicase activity toward DNA substrate is inhibited by micromolar concentrations of 5,6-dichloro-1-(beta-D-ribofuranosyl)benzotriazole (DRBT) and 4,5,6,7-tetrabromobenzotriazole (TBBT). Helicase activity toward RNA substrate is inhibited by elevated concentrations of TBBT. Inhibited by some ring-expanded nucleoside analogs.
Features
Showing features for binding site.
GO annotations
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent RNA helicase SUPV3L1, mitochondrial
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ80YD1
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Die in utero before midgestation. Show elevated sister chromatid exchange (SCE).
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 41 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for transit peptide, chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-40 | Mitochondrion | ||||
Sequence: MSLPRCTLLWARLPAGRGAGPRAAPCSALRALVGSFPGAS | ||||||
Chain | PRO_0000310546 | 41-779 | ATP-dependent RNA helicase SUPV3L1, mitochondrial | |||
Sequence: GRVPCLAASSSASGGSKAPNTSLFVPLTVKPQGPSADGDVGAELTRPLDKNEVKKILDKFYKRQEIQKLSADYGLDARLFHQAFISFRNYIMQSHSLDVDIHIVLNDICFSAAHVDDLFPFFLRHAKQIFPVLECKDDLRKISDLRIPPNWYPEARARQRKIIFHSGPTNSGKTYHAIQRYLSATSGVYCGPLKLLAHEIFEKSNAAGVPCDLVTGEERLTVEPEGKQATHVSCTVEMCNVATPYEVAVIDEIQMIRDPARGWAWTRALLGLCAEEVHLCGESAAINLVSELLYTTGEEVEVQKYERLTPISVLDHALESLDNLQPGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQARKFNDPNDPCKILVATDAIGMGLNLSIRRIIFYSLIKPSINEKGEKELEPITTSQALQIAGRAGRFSSHFKEGQVTTMHRDDLALLKDILNRPVDPIQAAGLHPTAEQIEMFAYHLPETTLSNLIDIFVDFAQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEAVHDVFDLYLWLSYRFIDMFPDSSLVRSLQKELDAIIQEGVHNITKLIKISESRKLLNLESLPSGDQSRLSGASKSPARRTRGTKSAGNKATEPLSPSDKELPLASRLVQQGLLTADMLRQLQKEWLTQQPEHSREKVGTRRKKKDPDSD | ||||||
Modified residue | 99 | N6-acetyllysine | ||||
Sequence: K | ||||||
Modified residue | 725 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Homodimer; in free form. Component of the mitochondrial degradosome (mtEXO) complex which is a heteropentamer containing 2 copies of SUPV3L1 and 3 copies of PNPT1. As part of mitochondrial degradosome complex, interacts with GRSF1 in a RNA-dependent manner; the interaction enhances the activity of the complex. Interacts with LAMTOR5/HBXIP, WRN and BLM.
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 194-334 | Helicase ATP-binding | ||||
Sequence: EARARQRKIIFHSGPTNSGKTYHAIQRYLSATSGVYCGPLKLLAHEIFEKSNAAGVPCDLVTGEERLTVEPEGKQATHVSCTVEMCNVATPYEVAVIDEIQMIRDPARGWAWTRALLGLCAEEVHLCGESAAINLVSELLY | ||||||
Domain | 353-521 | Helicase C-terminal | ||||
Sequence: VLDHALESLDNLQPGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQARKFNDPNDPCKILVATDAIGMGLNLSIRRIIFYSLIKPSINEKGEKELEPITTSQALQIAGRAGRFSSHFKEGQVTTMHRDDLALLKDILNRPVDPIQAAGLHPTAEQIEMFA | ||||||
Region | 650-779 | Interaction with LAMTOR5, important for protein stability | ||||
Sequence: PDSSLVRSLQKELDAIIQEGVHNITKLIKISESRKLLNLESLPSGDQSRLSGASKSPARRTRGTKSAGNKATEPLSPSDKELPLASRLVQQGLLTADMLRQLQKEWLTQQPEHSREKVGTRRKKKDPDSD | ||||||
Compositional bias | 693-722 | Polar residues | ||||
Sequence: SGDQSRLSGASKSPARRTRGTKSAGNKATE | ||||||
Region | 693-732 | Disordered | ||||
Sequence: SGDQSRLSGASKSPARRTRGTKSAGNKATEPLSPSDKELP | ||||||
Region | 754-779 | Disordered | ||||
Sequence: EWLTQQPEHSREKVGTRRKKKDPDSD | ||||||
Compositional bias | 758-779 | Basic and acidic residues | ||||
Sequence: QQPEHSREKVGTRRKKKDPDSD |
Sequence similarities
Belongs to the helicase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length779
- Mass (Da)87,005
- Last updated2003-06-01 v1
- Checksum877F2B611FEEE1A9
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
M0QWM4 | M0QWM4_MOUSE | Supv3l1 | 256 |
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 109 | in Ref. 1; CAI92124 | ||||
Sequence: L → R | ||||||
Sequence conflict | 157 | in Ref. 1; CAI92124 | ||||
Sequence: D → G | ||||||
Compositional bias | 693-722 | Polar residues | ||||
Sequence: SGDQSRLSGASKSPARRTRGTKSAGNKATE | ||||||
Compositional bias | 758-779 | Basic and acidic residues | ||||
Sequence: QQPEHSREKVGTRRKKKDPDSD |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ968954 EMBL· GenBank· DDBJ | CAI92124.1 EMBL· GenBank· DDBJ | mRNA | ||
BC049796 EMBL· GenBank· DDBJ | AAH49796.1 EMBL· GenBank· DDBJ | mRNA |