Q80VP1 · EPN1_MOUSE

Function

function

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity).
Regulates receptor-mediated endocytosis (By similarity).

Features

Showing features for binding site.

157550100150200250300350400450500550
TypeIDPosition(s)Description
Binding site11a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) (UniProtKB | ChEBI)
Binding site25a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) (UniProtKB | ChEBI)
Binding site30a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) (UniProtKB | ChEBI)
Binding site63a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) (UniProtKB | ChEBI)
Binding site73a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentclathrin vesicle coat
Cellular Componentclathrin-coated pit
Cellular Componentcytosol
Cellular Componentendosome
Cellular Componentnucleus
Cellular Componentplasma membrane
Cellular Componentpostsynapse
Cellular Componentpostsynaptic membrane
Cellular Componentpresynapse
Cellular Componentpresynaptic membrane
Cellular ComponentSchaffer collateral - CA1 synapse
Molecular Functionclathrin adaptor activity
Molecular Functionclathrin binding
Molecular Functionmolecular sequestering activity
Molecular Functionphospholipid binding
Molecular Functiontransmembrane transporter binding
Biological Processclathrin coat assembly
Biological Processembryonic organ development
Biological Processendocytosis
Biological Processfemale pregnancy
Biological Processin utero embryonic development
Biological Processmembrane fission
Biological Processnegative regulation of sprouting angiogenesis
Biological ProcessNotch signaling pathway
Biological Processpositive regulation of clathrin coat assembly

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Epsin-1
  • Alternative names
    • EPS-15-interacting protein 1
    • Intersectin-EH-binding protein 1 (Ibp1)

Gene names

    • Name
      Epn1

Organism names

  • Taxonomic identifier
  • Strain
    • FVB/N
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q80VP1
  • Secondary accessions
    • O70446
    • Q6NX78

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Cell membrane
; Peripheral membrane protein
Nucleus
Note: Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity).

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000745141-575Epsin-1
Modified residue382Phosphoserine
Modified residue418Phosphoserine
Modified residue419Phosphoserine
Modified residue420Phosphothreonine
Modified residue434Phosphoserine
Modified residue446Phosphoserine
Modified residue453Phosphoserine
Modified residue459Phosphothreonine
Modified residue463Phosphothreonine
Modified residue469Phosphothreonine
Modified residue472Phosphoserine
Modified residue493Phosphothreonine
Modified residue533Omega-N-methylarginine

Post-translational modification

Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.
Ubiquitinated.

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Monomer. Binds clathrin and ZBTB16/ZNF145 (By similarity).
Binds ubiquitinated proteins (By similarity).
Interacts with RALBP1 in a complex also containing NUMB and TFAP2A during interphase and mitosis (By similarity).
Interacts with AP2B1 (By similarity).
Interacts with UBQLN2 (By similarity).
Interacts with ITSN1. Interacts with AP2A1 and AP2A2. Interacts with REPS2; the interaction is direct (By similarity).
Interacts with EPS15; the interaction is direct (By similarity).
Interacts with ENTREP1 (By similarity).

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for domain, region, repeat, motif, compositional bias.

TypeIDPosition(s)Description
Domain12-144ENTH
Region150-186Disordered
Domain183-202UIM 1
Domain208-227UIM 2
Domain233-252UIM 3
Region264-575Disordered
Repeat274-2761
Region274-3798 X 3 AA repeats of D-P-W
Repeat294-2962
Repeat306-3083
Repeat319-3214
Repeat332-3345
Repeat349-3516
Repeat367-3697
Repeat377-3798
Motif401-410[DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif
Compositional bias455-469Pro residues
Repeat501-5031
Region501-5733 X 3 AA repeats of N-P-F
Compositional bias506-520Pro residues
Repeat517-5192
Compositional bias537-575Pro residues
Repeat571-5733

Domain

The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP2A2 and clathrin binding.
The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction the AP-2 complex subunit AP2B1.

Sequence similarities

Belongs to the epsin family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q80VP1-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    575
  • Mass (Da)
    60,212
  • Last updated
    2006-11-28 v3
  • Checksum
    70B8011EB3AE5C4C
MSTSSLRRQMKNIVHNYSEAEIKVREATSNDPWGPSSSLMSEIADLTYNVVAFSEIMSMIWKRLNDHGKNWRHVYKAMTLMEYLIKTGSERVSQQCKENMYAVQTLKDFQYVDRDGKDQGVNVREKAKQLVALLRDEDRLREERAHALKTKEKLAQTATASSAAVGSGPPPEAEQAWPQSSGEEELQLQLALAMSKEEADQPPSCGPEDDVQLQLALSLSREEHDKEERIRRGDDLRLQMAIEESKRETGGKEESSLMDLADVFTTPAPPQASDPWGGPASVPTAVPVAAAASDPWGGPAVPPAADPWGGAAPTPASGDPWRPAAPTGPSVDPWGGTPAPAAGEGPTPDPWGSSDGGAPVSGPPSSDPWAPAPAFSDPWGGSPAKPSSNGTAVGGFDTEPDEFSDFDRLRTALPTSGSSTGELELLAGEVPARSPGAFDMSGVGGSLAESVGSPPPAATPTPTPPTRKTPESFLGPNAALVDLDSLVSRPGPTPPGSKASNPFLPSGAPPTGPSVTNPFQPAPPATLTLNQLRLSPVPPVPGAPPTYISPLGGGPGLPPMMPPGPPAPNTNPFLL

Q80VP1-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
D3Z4V3D3Z4V3_MOUSEEpn190
D3Z550D3Z550_MOUSEEpn154

Features

Showing features for alternative sequence, compositional bias.

TypeIDPosition(s)Description
Alternative sequenceVSP_009153392in isoform 2
Compositional bias455-469Pro residues
Compositional bias506-520Pro residues
Compositional bias537-575Pro residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BC046962
EMBL· GenBank· DDBJ
AAH46962.2
EMBL· GenBank· DDBJ
mRNA
BC067206
EMBL· GenBank· DDBJ
AAH67206.1
EMBL· GenBank· DDBJ
mRNA
BC099682
EMBL· GenBank· DDBJ
AAH99682.1
EMBL· GenBank· DDBJ
mRNA
AF057285
EMBL· GenBank· DDBJ
AAC97475.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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