Q80RC7 · GCNT3_BHV4
- ProteinBeta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
- GeneBo17
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids439 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Non-essential glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans. Contributes to the post-translational modifications of structural proteins.
Miscellaneous
Was acquired from an ancestor of the African buffalo around 1.5 million years ago.
Catalytic activity
- O3-[beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + H+ + UDP
- 3-O-[N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-[N-acetyl-beta-D-glucosaminyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + H+ + UDP
- 3-O-[N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-[N-acetyl-beta-D-glucosaminyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + H+ + UDP
Pathway
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell Golgi membrane | |
Cellular Component | membrane | |
Molecular Function | acetylgalactosaminyl-O-glycosyl-seryl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity | |
Molecular Function | acetylgalactosaminyl-O-glycosyl-threonyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity | |
Molecular Function | beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity | |
Molecular Function | N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity | |
Biological Process | protein glycosylation |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameBeta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Peploviricota > Herviviricetes > Herpesvirales > Orthoherpesviridae > Gammaherpesvirinae > Rhadinovirus > Rhadinovirus bovinegamma4
- Virus hosts
Accessions
- Primary accessionQ80RC7
Subcellular Location
UniProt Annotation
GO Annotation
Host Golgi apparatus membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-11 | Cytoplasmic | ||||
Sequence: MVGWKKKKLCR | ||||||
Transmembrane | 12-29 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: GHHLWVLGCYMLLAVVSL | ||||||
Topological domain | 30-439 | Lumenal | ||||
Sequence: RLSLRFKCDVDSLDLESRDFQSQHCRDMLYNNLKLPAKRSINCSGITRGDQEAVVQALLDNLEVKKKRPPLTDTYYLNITRDCERFKAQRKFIQFPLSKEELDFPIAYSMVVHEKIENFERLLRAVYAPQNIYCVHVDVKSPETFKEAVKAIISCFPNVFMASKLVPVVYASWSRVQADLNCMEDLLQSSVSWKYLLNTCGTDFPIKTNAEMVLALKMLKGKNSMESEVPSESKKNRWKYHYEVTDTLYPTSKMKDPPPDNLPMFTGNAYFVASRAFVQHVLDNPKSQRLVEWVKDTYSPDEHLWATLQRAPWMPGSVPSHPKYHISDMTAVARLVKWQYHEGDVSMGAPYAPCSGIHRRAICIYGAGDLYWILQNHHLLANKFDPRVDDNVLQCLEEYLRHKAIYGTEL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000288551 | 1-439 | Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase | |||
Sequence: MVGWKKKKLCRGHHLWVLGCYMLLAVVSLRLSLRFKCDVDSLDLESRDFQSQHCRDMLYNNLKLPAKRSINCSGITRGDQEAVVQALLDNLEVKKKRPPLTDTYYLNITRDCERFKAQRKFIQFPLSKEELDFPIAYSMVVHEKIENFERLLRAVYAPQNIYCVHVDVKSPETFKEAVKAIISCFPNVFMASKLVPVVYASWSRVQADLNCMEDLLQSSVSWKYLLNTCGTDFPIKTNAEMVLALKMLKGKNSMESEVPSESKKNRWKYHYEVTDTLYPTSKMKDPPPDNLPMFTGNAYFVASRAFVQHVLDNPKSQRLVEWVKDTYSPDEHLWATLQRAPWMPGSVPSHPKYHISDMTAVARLVKWQYHEGDVSMGAPYAPCSGIHRRAICIYGAGDLYWILQNHHLLANKFDPRVDDNVLQCLEEYLRHKAIYGTEL | ||||||
Glycosylation | 71 | N-linked (GlcNAc...) asparagine; by host | ||||
Sequence: N | ||||||
Disulfide bond | 72↔229 | |||||
Sequence: CSGITRGDQEAVVQALLDNLEVKKKRPPLTDTYYLNITRDCERFKAQRKFIQFPLSKEELDFPIAYSMVVHEKIENFERLLRAVYAPQNIYCVHVDVKSPETFKEAVKAIISCFPNVFMASKLVPVVYASWSRVQADLNCMEDLLQSSVSWKYLLNTC | ||||||
Glycosylation | 107 | N-linked (GlcNAc...) asparagine; by host | ||||
Sequence: N | ||||||
Disulfide bond | 163↔383 | |||||
Sequence: CVHVDVKSPETFKEAVKAIISCFPNVFMASKLVPVVYASWSRVQADLNCMEDLLQSSVSWKYLLNTCGTDFPIKTNAEMVLALKMLKGKNSMESEVPSESKKNRWKYHYEVTDTLYPTSKMKDPPPDNLPMFTGNAYFVASRAFVQHVLDNPKSQRLVEWVKDTYSPDEHLWATLQRAPWMPGSVPSHPKYHISDMTAVARLVKWQYHEGDVSMGAPYAPC | ||||||
Disulfide bond | 184↔211 | |||||
Sequence: CFPNVFMASKLVPVVYASWSRVQADLNC | ||||||
Disulfide bond | 392↔424 | |||||
Sequence: CIYGAGDLYWILQNHHLLANKFDPRVDDNVLQC |
Keywords
- PTM
PTM databases
Expression
Developmental stage
Expressed during BHV-4 replication (at protein level).
Family & Domains
Sequence similarities
Belongs to the glycosyltransferase 14 family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length439
- Mass (Da)50,741
- Last updated2003-06-01 v1
- Checksum70E67B83A41A887D