Q80874 · VSR_BSMV
- ProteinSuppressor of RNA silencing
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids153 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs (PubMed:12438624).
Promotes viral cell-to-cell long distance movement by enhancing the ATPase activity of TGB1 (PubMed:12438624, PubMed:32730331).
Enhances RNA helicase activity of replication protein alpha-A (PubMed:28388677).
Suppresses autophagy induced by the host as a defense mechanism against viral infection (PubMed:29848767).
Promotes viral cell-to-cell long distance movement by enhancing the ATPase activity of TGB1 (PubMed:12438624, PubMed:32730331).
Enhances RNA helicase activity of replication protein alpha-A (PubMed:28388677).
Suppresses autophagy induced by the host as a defense mechanism against viral infection (PubMed:29848767).
Miscellaneous
The genome of this virus consists of three linear, positive, single-stranded RNAs encapsidated in separate virions designated RNA-alpha, RNA-beta and RNA-gamma. Three proteins (alpha-A, beta-A and gamma-A) are translated directly from these genomic RNAs and the remaining proteins encoded on RNA-beta (beta-B, beta-C and beta-D) and RNA-gamma (gamma-B) are expressed via three subgenomic messenger RNAs.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | host cell endoplasmic reticulum | |
Cellular Component | host cell plasmodesma | |
Molecular Function | metal ion binding | |
Biological Process | suppression by virus of host autophagy | |
Biological Process | symbiont-mediated suppression of host RNAi-mediated antiviral immune response | |
Biological Process | virus-mediated perturbation of host defense response |
Keywords
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameSuppressor of RNA silencing
- Alternative names
Organism names
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Kitrinoviricota > Alsuviricetes > Martellivirales > Virgaviridae > Hordeivirus
- Virus hosts
Accessions
- Primary accessionQ80874
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Recruited by the replication protein alpha-A to viral replication sites at the host chloroplast membrane.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 7 | Complete loss of C1 zinc-binding activity; when associated with S-9; S-10; S-19 and S-23. | |||
Mutagenesis | 9 | Complete loss of C1 zinc-binding activity; when associated with S-7; S-10; S-19 and S-23. | |||
Mutagenesis | 10 | Complete loss of C1 zinc-binding activity; when associated with S-7; S-9; S-19 and S-23. | |||
Mutagenesis | 19 | Complete loss of C1 zinc-binding activity; when associated with S-7; S-9; S-10 and S-23. | |||
Mutagenesis | 23 | Complete loss of C1 zinc-binding activity; when associated with S-7; S-9; S-10 and S-19. | |||
Mutagenesis | 25 | Complete loss of BM zinc-binding activity; when associated with K-26; E-33; K-35 and E-36. | |||
Mutagenesis | 26 | Complete loss of BM zinc-binding activity; when associated with E-25; E-33; K-35 and E-36. | |||
Mutagenesis | 33 | Complete loss of BM zinc-binding activity; when associated with E-25; K-26; K-35 and E-36. | |||
Mutagenesis | 35 | Complete loss of BM zinc-binding activity; when associated with E-25; K-26; E-33 and E-36. | |||
Mutagenesis | 36 | Complete loss of BM zinc-binding activity; when associated with E-25; K-26; E-33 and K-35. | |||
Mutagenesis | 60 | Complete loss of C2 zinc-binding activity; when associated with S-64; S-71; S-81 and S-85. | |||
Mutagenesis | 64 | Complete loss of C2 zinc-binding activity; when associated with S-60; S-71; S-81 and S-85. | |||
Mutagenesis | 71 | Complete loss of C2 zinc-binding activity; when associated with S-60; S-64; S-81 and S-85. | |||
Mutagenesis | 81 | Complete loss of C2 zinc-binding activity; when associated with S-60; S-64; S-71 and S-85. | |||
Mutagenesis | 85 | Complete loss of C2 zinc-binding activity; when associated with S-60; S-64; S-71 and S-81. | |||
Mutagenesis | 96 | Reduced viral accumulation. | |||
Mutagenesis | 96 | No effect on viral accumulation. | |||
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000222494 | 1-153 | Suppressor of RNA silencing | ||
Modified residue | 96 | Phosphoserine | |||
Post-translational modification
Phosphorylated at Ser-96 by a host PKA-like kinase; the phosphorylation at this site seems to suppress host cell death.
Serine-phosphorylated by host STY46 kinase.
Keywords
- PTM
PTM databases
Interaction
Subunit
Homooligomer (PubMed:29453938).
Interacts (via C-terminus) with replication protein alpha-A (PubMed:28388677).
Interacts (via N-terminus) with the movement protein TGB1; this interaction targets gammab-TGB1 at the periphery of chloroplasts and plasmodesmata (PubMed:32730331).
Interacts with host autophagy protein ATG7; this interaction disrupts the host ATG7-ATG8 interaction to promote viral infection (PubMed:29848767).
Interacts (via BM region) with host STY46; this interaction inhibits the viral infection (PubMed:33576790).
Interacts (via C-terminus) with replication protein alpha-A (PubMed:28388677).
Interacts (via N-terminus) with the movement protein TGB1; this interaction targets gammab-TGB1 at the periphery of chloroplasts and plasmodesmata (PubMed:32730331).
Interacts with host autophagy protein ATG7; this interaction disrupts the host ATG7-ATG8 interaction to promote viral infection (PubMed:29848767).
Interacts (via BM region) with host STY46; this interaction inhibits the viral infection (PubMed:33576790).
Structure
Family & Domains
Features
Showing features for region, coiled coil.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-23 | C-1 | |||
Region | 1-85 | Interaction with TGB1 | |||
Region | 19-47 | Basic motif (BM) | |||
Region | 60-85 | C-2 | |||
Region | 86-127 | Interaction with replication protein alpha-A | |||
Coiled coil | 92-132 | ||||
Domain
The three domains C1, BM and C2 are involved in zinc-binding. Zinc-binding of each of the motifs is critical for the biological activity.
The coiled coil domain is probably involved in homooligomerization.
Sequence similarities
Belongs to the virgaviridae suppressor of RNA silencing family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length153
- Mass (Da)17,160
- Last updated1996-11-01 v1
- Checksum979B4CC3F451EA97
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 54 | in Ref. 2; CAA36984 | |||
Sequence conflict | 63 | in Ref. 2; CAA36984 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M16576 EMBL· GenBank· DDBJ | AAA66601.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
X52774 EMBL· GenBank· DDBJ | CAA36984.1 EMBL· GenBank· DDBJ | Genomic RNA |