Q806Y6 · L_DOBV
- ProteinRNA-directed RNA polymerase L
- GeneL
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids2151 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
RNA-dependent RNA polymerase, which is responsible for the replication and transcription of the viral RNA genome using antigenomic RNA as an intermediate (By similarity).
During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, which involves the endonuclease activity cleaving the host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription. Cleaves ssRNA substrates but not DNA (By similarity).
Seems to downregulate the expression of its own and heterologous mRNAs through its endonuclease activity (By similarity).
During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, which involves the endonuclease activity cleaving the host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription. Cleaves ssRNA substrates but not DNA (By similarity).
Seems to downregulate the expression of its own and heterologous mRNAs through its endonuclease activity (By similarity).
Miscellaneous
Classified as His+ endonuclease since it has a histidine upstream of the active site that coordinates the first cation.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Cofactor
Protein has several cofactor binding sites:
Note: For endonuclease activity. Binds 2 Mn2+ ions in the active site. The divalent metal ions are crucial for catalytic activity (PubMed:31948728).
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: For polymerase activity.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 36 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 54 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 97 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 97 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 110 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 111 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Active site | 124 | For endonuclease activity | ||||
Sequence: K | ||||||
Binding site | 1099 | Mg2+ (UniProtKB | ChEBI); catalytic; for RdRp activity | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell perinuclear region of cytoplasm | |
Molecular Function | endonuclease activity | |
Molecular Function | metal ion binding | |
Molecular Function | nucleotide binding | |
Molecular Function | RNA-dependent RNA polymerase activity | |
Biological Process | cap snatching | |
Biological Process | DNA-templated transcription | |
Biological Process | viral RNA genome replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRNA-directed RNA polymerase L
- EC number
- Short namesProtein L
- Alternative names
Including 1 domain:
- Recommended namecap-snatching endonuclease
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Negarnaviricota > Polyploviricotina > Ellioviricetes > Bunyavirales > Hantaviridae > Mammantavirinae > Orthohantavirus
- Virus hosts
Accessions
- Primary accessionQ806Y6
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000455191 | 1-2151 | RNA-directed RNA polymerase L | |||
Sequence: MEKYREIHRDLQSFPVGSLTAVECIDYLDRLYAIRHDIVDQMIKHDWSDNKDSEESIGKVLLFAGVPNNVITAMEKKIIPDHPSGKTLRSFFKMTPDNYKITGSTIEFVEVTVTVDVDKGIREKRLKYEAGLKYIEQELHNHFLRGDIPQPYKITFQVVSVRTDGSNISTQWPSRRNDGVVQYMRLVQAEISYVREHLIRQEERAALEAMFNLKFNISNIKNQPYYIPDYRGIPLIHPNINDLVVYMRDWLSKSHKFSFHEGKVPAVFDCFNENELEHAVKYPISRHPRNFLLIQCSLLSSYNPATILSDQVDSRRACNSVLNLIPETPTSFLIHDMAYRYINLTREDMVSFYAPKTQFIPTQNVKEPGTFKLTANSMRPESKAMLDMLGSHEPGEKKGALIESLNLSSHIVQSECVSLITKILSDLELNISEPTSHGSFTTKHTYVDNVLEKFFQNEIQRYLLDVLKKTTAWHIGHLIRDITESLIAHSGLKRSKYWSVHAYNNGNVILFILPSKSLEVAGSYIRFVTVFRMGPGLVDKDNLDTILTDQDVTWGVSKVMSIDLNRLLALNIAFEKALIATATWFQYYTEDQGQFPLQHAIRSVFAYHLLLAVCQKMKLCAIFDNLRYLIPAVTSLYSGFPSLINKLFERPFKSALEVYVYYNIKSLLVALAQNNKARFYSKVKLLGLTVDQSTVGASGIYPSFMSRVIYKHYRSLISEVTTCFFLFEKGLHGNMNEEAKIHLETVEWALKFRQKEDQYGESMVENGYTIGELNDNQDLVEQQLYCQDAVELAAVELNKILSTKSQVVANSILNKYWEVPYFSQTRNISLKGMSGQVQEDGHLAASVTIIEAIRYLSSSQNNPSVLQLYEETRKVKAQARIVRKYQRTEADRGFFITTLPTRCRLEIIEDYYDAISKNVAEEYISYGGERKILCIQSALEKALRWASGESFIELSNGKFIRMKRKLMYVSADATKWSPGDNSAKFRRFTAALHNGLPDDRLKNCVIDALRNVYKTDFYMSRKLRAYIDNMNGHEPAVKNFLEFFPDGHCGEVRGNWLQGNLNKCSSLFGVGMSLLFKQLWNELFPELDCFFEFAHHSDDALFIYGYLEPIDDGTDWFLYVSQQIQAGHLHWFSVNTEMWKSMFNLHEHVLLLGSIKISPKKTTLSPTNAEFLSTFFESCAVSIPFIKILLGSLSDLPGLGYFDDLAAAQSRCVKAMDLGASPQVAQLAVALCTNKVERLYGTAVGMIKHPSTYLQVKHGDTPIPLGGSGAMSIMELATAGIGMSDKNLLKRALLGYIHKRQKNMAYILGLFKFLMNLSKDTFQHERLGEFSFIGKVQWKIFIPKSEFEFFDMYTPKFLKLWSEQHVTYDYIIPKGRDNLLIYLVRKINDPSIVTAMTMQSPLQLRFRMQAKQHMKVCKLDDDWVTFREILAAANSFAQLYEVTQEDLDLFQTLTSCTFSKEYAWKDFLNGVQCDVIPTKQIQRAKVARTFTVREKDQIIQNSIPAVIGYKFAVTVDEMSDVLDSAKFPDSLAVDLKTMKDGVYRELGLDISQAEVMKKVAPMLYKSSKSRVVIVQGNVEGTAEAICGYWLRTMSLVKTIRVKPHKEVLKAVSIFNRKEDIGQQKDLAALRLCIEVWRWCKANNAPYQEWFHALWFEDKTFSEWLDRFIRVGVPPVDPEIQCAALMIADIRGDLSVLQVQANRRAYSGKQYDAYCVQTYNEETKLYEGDLRVTFNFGLDCARLEIFWDKQTYILETSITQKHVLKIMMDEVTKELLRCGMRFKTEQVSNVKHLVLFKTEAGFEWGKPNIPCIVYKNCALRTGLRANQTVNHKFMISIKDNGLRAIAQYDDESPRFLLAHAFHTIRDIRYQAVDAVSNVWFIHKGIKLFLNPIISSGLLENFMKNLPAAIPPAAYSLIMNRAKISVDLFMFNDLLRLINPSNTLDLSGLQPTEDGFSTVSSMSSRLWSEEVSFVDEDEEIDDEFTIDLQDVDFENIDVEADIEHFLQDESSYTGDLLIMSEETEVKKMRGIIKLLEPVRLIKSWVSKGLCIEKVYSPTNIILMTRYLSKNFNFSGRQVSLLDPYDLTEFESIVKGWGECVVDQFSTFDQETQLLVSQKGICPEDVVPDSLFSFRHTIVLLRRLFPQDSVSTFY |
Interaction
Subunit
Interacts with the viral nucleoprotein.
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 956-1142 | RdRp catalytic | ||||
Sequence: NGKFIRMKRKLMYVSADATKWSPGDNSAKFRRFTAALHNGLPDDRLKNCVIDALRNVYKTDFYMSRKLRAYIDNMNGHEPAVKNFLEFFPDGHCGEVRGNWLQGNLNKCSSLFGVGMSLLFKQLWNELFPELDCFFEFAHHSDDALFIYGYLEPIDDGTDWFLYVSQQIQAGHLHWFSVNTEMWKSM |
Domain
The N-terminus contains the endonuclease activity (endoN) (By similarity).
The central region contains the RdRp activity (By similarity).
The C-terminus contains the cap-binding region (By similarity).
The central region contains the RdRp activity (By similarity).
The C-terminus contains the cap-binding region (By similarity).
Sequence similarities
Belongs to the Bunyavirales RNA polymerase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length2,151
- Mass (Da)246,532
- Last updated2003-06-01 v1
- Checksum61231C9463B71742
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ410617 EMBL· GenBank· DDBJ | CAC85167.1 EMBL· GenBank· DDBJ | Genomic RNA |