Q7ZZX9 · RAN_CARAU

Function

function

GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation. Required for normal progress through mitosis.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Mg2+ interacts primarily with the phosphate groups of the bound guanine nucleotide.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site17-24GTP (UniProtKB | ChEBI)
Binding site35-41GTP (UniProtKB | ChEBI)
Binding site67GTP (UniProtKB | ChEBI)
Site68Essential for GTP hydrolysis
Binding site121-124GTP (UniProtKB | ChEBI)
Binding site149-151GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentnuclear envelope
Cellular Componentnucleus
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionmagnesium ion binding
Biological ProcessGTP metabolic process
Biological Processmitotic sister chromatid segregation
Biological Processprotein import into nucleus
Biological ProcesssnRNA import into nucleus

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    GTP-binding nuclear protein Ran
  • EC number
  • Alternative names
    • GTPase Ran
    • Ras-related nuclear protein

Gene names

    • Name
      ran

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Cyprinidae > Cyprininae > Carassius

Accessions

  • Primary accession
    Q7ZZX9

Proteomes

Subcellular Location

Nucleus
Nucleus envelope
Cytoplasm, cytosol
Cytoplasm
Note: Predominantly nuclear during interphase. Becomes dispersed throughout the cytoplasm during mitosis (By similarity).

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002087051-215GTP-binding nuclear protein Ran

Interaction

Subunit

Monomer. Interacts with RANGAP1, which promotes RAN-mediated GTP hydrolysis. Interacts with KPNB1. Interaction with KPNB1 inhibits RANGAP1-mediated stimulation of GTPase activity. Interacts with RCC1 which promotes the exchange of RAN-bound GDP by GTP. Interaction with KPNB1 inhibits RCC1-mediated exchange of RAN-bound GDP by GTP. Interacts (GTP-bound form) with TNPO1; the interaction is direct. Interacts (GTP-bound form) with TNPO3; the interaction is direct. Interacts with KPNB1 and with TNPO1; both inhibit RAN GTPase activity. Interacts (via C-terminus) with RANBP1, which alleviates the inhibition of RAN GTPase activity. Interacts with RANGRF, which promotes the release of bound guanine nucleotide. RANGRF and RCC1 compete for an overlapping binding site on RAN. Identified in a complex with KPNA2 and CSE1L; interaction with RANBP1 mediates dissociation of RAN from this complex. Interaction with both RANBP1 and KPNA2 promotes dissociation of the complex between RAN and KPNB1. Identified in a complex composed of RAN, RANGAP1 and RANBP1. Identified in a complex that contains TNPO1, RAN and RANBP1. Identified in a nuclear export complex with XPO1. Interaction with RANBP1 or RANBP2 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. Component of a nuclear export receptor complex composed of KPNB1, RAN, SNUPN and XPO1.

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region210-215Interaction with RANBP1

Sequence similarities

Belongs to the small GTPase superfamily. Ran family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    215
  • Mass (Da)
    24,462
  • Last updated
    2003-06-01 v1
  • Checksum
    CF3B5CF25DE6A6CB
MAENEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKYNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVEMSALAPPEIAMDPSLAAQYEHDLKVASETALPDEDDDL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY248751
EMBL· GenBank· DDBJ
AAP03080.1
EMBL· GenBank· DDBJ
mRNA

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