Q7ZU13 · SPNS1_DANRE
- ProteinProtein spinster homolog 1
- Genespns1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids506 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Mediates the rate-limiting, proton-dependent, lysosomal efflux of lysophospholipids (PubMed:36161949).
Selective for zwitterionic headgroups such as lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE) (PubMed:36161949).
Essential player in lysosomal homeostasis (PubMed:27875093).
Critical for embryogenesis (PubMed:11836794, PubMed:18704191).
Involved in the regulation of developmental senescence (PubMed:27875093).
Selective for zwitterionic headgroups such as lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE) (PubMed:36161949).
Essential player in lysosomal homeostasis (PubMed:27875093).
Critical for embryogenesis (PubMed:11836794, PubMed:18704191).
Involved in the regulation of developmental senescence (PubMed:27875093).
Catalytic activity
- a 1-acyl-sn-glycero-3-phosphocholine(out) + H+(out) = a 1-acyl-sn-glycero-3-phosphocholine(in) + H+(in)
- a 1-acyl-sn-glycero-3-phosphoethanolamine(out) + H+(out) = a 1-acyl-sn-glycero-3-phosphoethanolamine(in) + H+(in)
- 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine(out) + H+(out) = 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine(in) + H+(in)
- 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine(out) + H+(out) = 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine(in) + H+(in)
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | lysosomal membrane | |
Cellular Component | membrane | |
Molecular Function | transmembrane transporter activity | |
Biological Process | autophagosome maturation | |
Biological Process | autophagy | |
Biological Process | cellular senescence | |
Biological Process | lipid transport |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameProtein spinster homolog 1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio
Accessions
- Primary accessionQ7ZU13
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Lysosome membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 52-71 | Helical | ||||
Sequence: IVLCYINLLNYMDRFTVAGV | ||||||
Transmembrane | 87-107 | Helical | ||||
Sequence: GLLQTVFICSYMFLAPLFGYL | ||||||
Transmembrane | 115-135 | Helical | ||||
Sequence: LIMCVGIFFWSVVTLASSFIG | ||||||
Transmembrane | 149-169 | Helical | ||||
Sequence: VGVGEASYSTIAPTIIADLFV | ||||||
Transmembrane | 176-196 | Helical | ||||
Sequence: MLSIFYFAIPVGSGMGYIVGS | ||||||
Transmembrane | 207-227 | Helical | ||||
Sequence: WALRVTPGLGLLAVFLLMLVV | ||||||
Transmembrane | 266-286 | Helical | ||||
Sequence: FGFTAVAFVTGSLALWAPAFL | ||||||
Transmembrane | 310-330 | Helical | ||||
Sequence: LIFGAITVVTGILGVASGVQA | ||||||
Transmembrane | 344-364 | Helical | ||||
Sequence: LVCAAGLLLAAPFLYLSIMFA | ||||||
Transmembrane | 373-393 | Helical | ||||
Sequence: VFIFLGETFLSMNWAIVADIL | ||||||
Transmembrane | 408-428 | Helical | ||||
Sequence: FQIVLSHLLGDAISPYLIGVV | ||||||
Transmembrane | 450-470 | Helical | ||||
Sequence: LLCSFVAVAGGAFFLATAVFI |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Presence in early embryos of an opaque substance in the posterior yolk cell extension at approximately 1 day after fertilization. This material progressively accumulates and by 48 hours after fertilization fills the entire yolk. By day 3 of embryogenesis, embryos are severely reduced in size compared with their wild-type siblings and they die a few hours later.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000305041 | 1-506 | Protein spinster homolog 1 | |||
Sequence: MSQADADITPFFADDNEGEGPVENGVGSPLPEDEEEESPSGVTDRRAIMTVIVLCYINLLNYMDRFTVAGVLPDIEHFFGIGDGTSGLLQTVFICSYMFLAPLFGYLGDRYNRKLIMCVGIFFWSVVTLASSFIGKDHFWALLLTRGLVGVGEASYSTIAPTIIADLFVKEKRTNMLSIFYFAIPVGSGMGYIVGSKVDTVAKDWHWALRVTPGLGLLAVFLLMLVVQEPKRGAIEAHPEHTLHRTSWLADMKALCRNPSFILSTFGFTAVAFVTGSLALWAPAFLFRAGVFTGVKQPCFKAPCDDSDSLIFGAITVVTGILGVASGVQASKLLRTRTPRADPLVCAAGLLLAAPFLYLSIMFAQASTVATYVFIFLGETFLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAISPYLIGVVSDSIKESNSYMWEFRSLQMSLLLCSFVAVAGGAFFLATAVFIEKDRDLAENYVPSDDAPIVVPRSGRSTKVSVSSVLI |
Proteomic databases
Expression
Tissue specificity
Expressed in yolk cells.
Developmental stage
Expressed both maternally and zygotically throughout embryogenesis, and also in adults.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-42 | Disordered | ||||
Sequence: MSQADADITPFFADDNEGEGPVENGVGSPLPEDEEEESPSGV |
Sequence similarities
Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length506
- Mass (Da)54,981
- Last updated2003-06-01 v1
- ChecksumB4168E84D2CC19BF
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
G1K2F4 | G1K2F4_DANRE | spns1 | 504 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 362 | in Ref. 1; AAL69987 | ||||
Sequence: M → I |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF465772 EMBL· GenBank· DDBJ | AAL69987.1 EMBL· GenBank· DDBJ | mRNA | ||
BC048024 EMBL· GenBank· DDBJ | AAH48024.1 EMBL· GenBank· DDBJ | mRNA |