Q7YJT0 · NDHI_CALFG
- ProteinNAD(P)H-quinone oxidoreductase subunit I, chloroplastic
- GenendhI
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids180 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient.
Catalytic activity
- a plastoquinone + NADH + (n+1) H+(in) = a plastoquinol + NAD+ + n H+(out)
a plastoquinone RHEA-COMP:9562 + CHEBI:57945 + (n+1) H+ (in)CHEBI:15378= a plastoquinol RHEA-COMP:9561 + CHEBI:57540 + n H+ (out)CHEBI:15378 - a plastoquinone + NADPH + (n+1) H+(in) = a plastoquinol + NADP+ + n H+(out)
Cofactor
Note: Binds 2 [4Fe-4S] clusters per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 64 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 67 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 70 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 74 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 104 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 107 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 110 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | |||
Binding site | 114 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast thylakoid membrane | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | iron ion binding | |
Molecular Function | NADH dehydrogenase (ubiquinone) activity | |
Molecular Function | quinone binding | |
Biological Process | photosynthesis, light reaction |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNAD(P)H-quinone oxidoreductase subunit I, chloroplastic
- EC number
- Alternative names
Gene names
Encoded on
- Chloroplast
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Magnoliidae > Laurales > Calycanthaceae > Calycanthus
Accessions
- Primary accessionQ7YJT0
Subcellular Location
UniProt Annotation
GO Annotation
Plastid, chloroplast thylakoid membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000245654 | 1-180 | NAD(P)H-quinone oxidoreductase subunit I, chloroplastic | ||
Keywords
- PTM
Interaction
Subunit
NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 55-84 | 4Fe-4S ferredoxin-type 1 | |||
Domain | 95-124 | 4Fe-4S ferredoxin-type 2 | |||
Sequence similarities
Belongs to the complex I 23 kDa subunit family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length180
- Mass (Da)21,005
- Last updated2003-10-01 v1
- ChecksumD661A9FCC6BE44A5
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ428413 EMBL· GenBank· DDBJ | CAD28776.1 EMBL· GenBank· DDBJ | Genomic DNA |