Q7XN16 · Q7XN16_ORYSJ
- ProteinDNA replication ATP-dependent helicase/nuclease
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1287 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Key enzyme involved in DNA replication and DNA repair. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA.
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Cofactor
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chromosome | |
Cellular Component | membrane | |
Cellular Component | nucleus | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | 5'-flap endonuclease activity | |
Molecular Function | ATP binding | |
Molecular Function | DNA binding | |
Molecular Function | metal ion binding | |
Molecular Function | single-stranded DNA helicase activity | |
Biological Process | DNA repair | |
Biological Process | DNA replication, Okazaki fragment processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA replication ATP-dependent helicase/nuclease
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ7XN16
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 87-108 | Helical | ||||
Sequence: LWRRSGSGFLLGWFGGFLFGAV |
Keywords
- Cellular component
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 255-291 | Disordered | ||||
Sequence: LPADGENKGEQLINGDPKNISCSPIADSDQNVPSQKS | ||||||
Compositional bias | 268-291 | Polar residues | ||||
Sequence: NGDPKNISCSPIADSDQNVPSQKS | ||||||
Domain | 329-528 | DNA replication factor Dna2 N-terminal | ||||
Sequence: EHGGKEHAVHLCDEWFHSLISPGDTVSVIGEFTDHGICIIDHDKNLVIVHPELLISGTRVASSFHCPRRSVLDDRLKSNEYSTSALIGTLLHQVFQAGLLEDVPSTQFLEQQAKEVLLKNIESLYACGASESNTLKTLIEAVPKILNWYKCFMKVSKCNNVNFGQIEGQKTVGVVEVMDIEEMAWAPRYGLKGIIDASIR | ||||||
Domain | 867-958 | DNA2/NAM7 helicase helicase | ||||
Sequence: NLNNDQQRSLHKILAAKDYALILGMPGTGKTYTMVHAVKSLLMRGESILLTSYTNSAIDNLLMKLKAEGVDFLRIGRHEAVHPDVKGHCLST | ||||||
Domain | 971-1035 | DNA2/NAM7 helicase helicase | ||||
Sequence: MEQVQVVGVTCLGIYHPLLAHKKFDTCIMDEAGQITLPVSLGPLMLATKFVLVGDHYQLPPLVQS | ||||||
Domain | 1044-1243 | DNA2/NAM7 helicase-like C-terminal | ||||
Sequence: GVSLFWRLSEAHPQAISALRCQYRMSSGIMELSNSLIYGNRLSCGSLEIANAKLKLSGRGPVRLKLKEILNPDRAVVFANTDEVPAFEAKEHRTVNNPTEALIVSWITNELLKRGVAQDGVGIITPYNAQANLIQQHVDASVEVHTIDKYQGRDKECIIVSFVRSNGNTRASGSSLLGDWHRINVVLTRAKKKLIMVGSA |
Sequence similarities
Belongs to the DNA2/NAM7 helicase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,287
- Mass (Da)141,977
- Last updated2003-10-01 v1
- Checksum774DF4E94CDDF9F2
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 268-291 | Polar residues | ||||
Sequence: NGDPKNISCSPIADSDQNVPSQKS |