Q7XN16 · Q7XN16_ORYSJ

Function

function

Key enzyme involved in DNA replication and DNA repair. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentchromosome
Cellular Componentmembrane
Cellular Componentnucleus
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function5'-flap endonuclease activity
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular Functionmetal ion binding
Molecular Functionsingle-stranded DNA helicase activity
Biological ProcessDNA repair
Biological ProcessDNA replication, Okazaki fragment processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA replication ATP-dependent helicase/nuclease
  • EC number

Gene names

    • ORF names
      OSJNBb0016D16.17
    • Ordered locus names
      Os04g0588200

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Nipponbare
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa

Accessions

  • Primary accession
    Q7XN16
  • Secondary accessions
    • Q0JAM9

Proteomes

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane87-108Helical

Keywords

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region255-291Disordered
Compositional bias268-291Polar residues
Domain329-528DNA replication factor Dna2 N-terminal
Domain867-958DNA2/NAM7 helicase helicase
Domain971-1035DNA2/NAM7 helicase helicase
Domain1044-1243DNA2/NAM7 helicase-like C-terminal

Sequence similarities

Belongs to the DNA2/NAM7 helicase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,287
  • Mass (Da)
    141,977
  • Last updated
    2003-10-01 v1
  • Checksum
    774DF4E94CDDF9F2
MAPRKRTGAAGWGACFEEACGLAAVAAGQVRHSPLLRAPVPGLLPERQAPEGRRPLAASRCLPTCLRRHRRSSRRSHRRCRRRSPRLWRRSGSGFLLGWFGGFLFGAVQLIKQSQDDGGGEVVTWKISPVNDRLRMVTSRQMTGMALHPCSNNEKHSSVESTKKWHSSLLGMSRCTASARNLDMCGTGPGGCDGVEDTQSPFRTPPSLSYRCSEQQLNGGVVSDGGPEQLGAGEHKKALLDLLDQVEDAIMEEELPADGENKGEQLINGDPKNISCSPIADSDQNVPSQKSLDAPPFDSFLVLEVSEKHKGDNLPCDRYPVKVLRLLNEHGGKEHAVHLCDEWFHSLISPGDTVSVIGEFTDHGICIIDHDKNLVIVHPELLISGTRVASSFHCPRRSVLDDRLKSNEYSTSALIGTLLHQVFQAGLLEDVPSTQFLEQQAKEVLLKNIESLYACGASESNTLKTLIEAVPKILNWYKCFMKVSKCNNVNFGQIEGQKTVGVVEVMDIEEMAWAPRYGLKGIIDASIRSRVSCNGSSYDRVMPLEFKTGKGTSGQTAVEHTAQVILYTLLMTERYLNKDIDLGLLYYLHTDQTLGIKVKRSDLIGLIMRRNELATEILKASISQSFPPMIQAHGGNAATSGLGDLFDNLVNHLTVAHHTFLKHWDRLIDLEARVSQVKKKEIFQPHHSNTGSRHSSPSYFVLDVTNGHSIDSSGKSKRYIYTFLRQKLQPETADQPGEHVESVDFSLKCGDSVVLSTKSGRIAVANGYIRDLSRAHITVSLPRRLRLPGSNSLLEQGDLQCEVWRIDKDESVSSFATMRLNLVQLFAENPQNSHLRKLIVDLEAPRFDSGGLLSQDPALSYIQSLPNLNNDQQRSLHKILAAKDYALILGMPGTGKTYTMVHAVKSLLMRGESILLTSYTNSAIDNLLMKLKAEGVDFLRIGRHEAVHPDVKGHCLSTTDIQSVDAVKARMEQVQVVGVTCLGIYHPLLAHKKFDTCIMDEAGQITLPVSLGPLMLATKFVLVGDHYQLPPLVQSSEARENGMGVSLFWRLSEAHPQAISALRCQYRMSSGIMELSNSLIYGNRLSCGSLEIANAKLKLSGRGPVRLKLKEILNPDRAVVFANTDEVPAFEAKEHRTVNNPTEALIVSWITNELLKRGVAQDGVGIITPYNAQANLIQQHVDASVEVHTIDKYQGRDKECIIVSFVRSNGNTRASGSSLLGDWHRINVVLTRAKKKLIMVGSAATLSTIPLLRLMIEKVAERGGQLDLTNKDVQSIRELTSSRLNVQ

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias268-291Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP008210
EMBL· GenBank· DDBJ
BAF15608.2
EMBL· GenBank· DDBJ
Genomic DNA
AL606658
EMBL· GenBank· DDBJ
CAE04326.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp