Q7XKV4 · BGL12_ORYSJ
- ProteinBeta-glucosidase 12
- GeneBGLU12
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids510 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, cello-oligosaccharides and laminaribiose.
Catalytic activity
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.56 mM | p-nitrophenyl beta-D-glucopyranoside | |||||
1.64 mM | p-nitrophenyl beta-D-thioglucoside | |||||
0.91 mM | n-heptyl-beta-D-glucoside | |||||
0.44 mM | n-octyl-beta-D-glucoside | |||||
1.61 mM | n-octyl-beta-D-thioglucopyranoside |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
20.1 μmol/min/mg | with p-nitrophenyl beta-D-glucopyranoside as substrate | ||||
0.29 μmol/min/mg | with p-nitrophenyl beta-D-thioglucoside as substrate | ||||
13.2 μmol/min/mg | with n-heptyl-beta-D-glucoside as substrate | ||||
10.7 μmol/min/mg | with n-octyl-beta-D-glucoside as substrate | ||||
4 μmol/min/mg | with n-octyl-beta-D-thioglucopyranoside as substrate |
kcat is 18.5 sec-1 with p-nitrophenyl beta-D-glucopyranoside as substrate. kcat is 0.27 sec-1 with p-nitrophenyl beta-D-thioglucoside as substrate. kcat is 12.1 sec-1 with n-heptyl-beta-D-glucoside as substrate. kcat is 9.8 sec-1 with n-octyl-beta-D-glucoside as substrate. kcat is 0.038 sec-1 n-octyl-beta-D-thioglucopyranoside as substrate.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 53 | a beta-D-glucoside (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 157 | a beta-D-glucoside (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 202-203 | a beta-D-glucoside (UniProtKB | ChEBI) | ||||
Sequence: NE | ||||||
Active site | 203 | Proton donor | ||||
Sequence: E | ||||||
Binding site | 346 | a beta-D-glucoside (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Active site | 417 | Nucleophile | ||||
Sequence: E | ||||||
Binding site | 417 | a beta-D-glucoside (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 466 | a beta-D-glucoside (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Binding site | 473-474 | a beta-D-glucoside (UniProtKB | ChEBI) | ||||
Sequence: EW | ||||||
Binding site | 482 | a beta-D-glucoside (UniProtKB | ChEBI) | ||||
Sequence: F |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | beta-D-fucosidase activity | |
Molecular Function | beta-galactosidase activity | |
Molecular Function | beta-gentiobiose beta-glucosidase activity | |
Molecular Function | beta-glucosidase activity | |
Molecular Function | beta-L-arabinosidase activity | |
Molecular Function | glucan exo-1,3-beta-glucosidase activity | |
Molecular Function | scopolin beta-glucosidase activity | |
Biological Process | carbohydrate metabolic process |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameBeta-glucosidase 12
- EC number
- Short namesOs4bglu12
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ7XKV4
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 276 | Decreases the kcat/Km values 2 to 6-fold depending on the substrate. | ||||
Sequence: H → M |
PTM/Processing
Features
Showing features for signal, chain, glycosylation, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-24 | |||||
Sequence: MAAAGAMPGGLLLTFLLLAVVASG | ||||||
Chain | PRO_0000390329 | 25-510 | Beta-glucosidase 12 | |||
Sequence: AYNGAGEPPVSRRSFPKGFIFGTASSSYQYEGGAAEGGRGPSIWDTFTHQHPEKIADRSNGDVASDSYHLYKEDVRLMKDMGMDAYRFSISWTRILPNGSLRGGVNKEGIKYYNNLINELLSKGVQPFITLFHWDSPQALEDKYNGFLSPNIINDFKDYAEICFKEFGDRVKNWITFNEPWTFCSNGYATGLFAPGRCSPWEKGNCSVGDSGREPYTACHHQLLAHAETVRLYKAKYQALQKGKIGITLVSHWFVPFSRSKSNDDAAKRAIDFMFGWFMDPLIRGDYPLSMRGLVGNRLPQFTKEQSKLVKGAFDFIGLNYYTANYADNLPPSNGLNNSYTTDSRANLTGVRNGIPIGPQAASPWLYVYPQGFRDLLLYVKENYGNPTVYITENGVDEFNNKTLPLQEALKDDARIEYYHKHLLSLLSAIRDGANVKGYFAWSLLDNFEWSNGYTVRFGINFVDYNDGRKRYPKNSAHWFKKFLLK | ||||||
Glycosylation | 122 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 208↔243 | |||||
Sequence: CSNGYATGLFAPGRCSPWEKGNCSVGDSGREPYTAC | ||||||
Disulfide bond | 222↔230 | |||||
Sequence: CSPWEKGNC | ||||||
Glycosylation | 229 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 361 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 371 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 425 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Protein-protein interaction databases
Structure
Sequence & Isoform
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 2 isoforms produced by Alternative splicing.
Q7XKV4-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length510
- Mass (Da)57,464
- Last updated2004-03-01 v2
- Checksum5828169EA16037DA
Q7XKV4-2
- Name2
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0P0WB96 | A0A0P0WB96_ORYSJ | Os04g0474800 | 222 | ||
A0A0P0WBJ3 | A0A0P0WBJ3_ORYSJ | Os04g0474800 | 268 |
Sequence caution
Features
Showing features for alternative sequence.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL731582 EMBL· GenBank· DDBJ | CAE05483.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP008210 EMBL· GenBank· DDBJ | BAF14984.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AP014960 EMBL· GenBank· DDBJ | BAS89674.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CM000141 EMBL· GenBank· DDBJ | EEE61179.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK100820 EMBL· GenBank· DDBJ | BAG94781.1 EMBL· GenBank· DDBJ | mRNA |