Q7X9V2 · PIE1_ARATH
- ProteinProtein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1
- GenePIE1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids2055 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant H2A.F/Z leading to transcriptional regulation of selected genes (e.g. FLC) by chromatin remodeling. Probable DNA-dependent ATPase. Not involved in the repression of FLC in gametophytes, but required for the reactivation of FLC in early embryos and for the maintenance of full activation of FLC in late embryos.
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plant-type cell wall | |
Cellular Component | SWI/SNF complex | |
Cellular Component | Swr1 complex | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | ATP-dependent chromatin remodeler activity | |
Molecular Function | DNA binding | |
Molecular Function | helicase activity | |
Biological Process | cell differentiation | |
Biological Process | flower development | |
Biological Process | response to cadmium ion |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ7X9V2
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Early flowering. Loss of H2A.Z from chromatin.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 149 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000423729 | 1-2055 | Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 | |||
Sequence: MASKGGKSKPDIVMASKSGKSKPDNESRAKRQKTLEAPKEPRRPKTHWDHVLEEMAWLSKDFESERKWKLAQAKKVALRASKGMLDQASREERKLKEEEQRLRKVALNISKDMKKFWMKVEKLVLYKHQLVRNEKKKKAMDKQLEFLLGQTERYSTMLAENLVEPYKQGQNTPSKPLLTIESKSDEERAEQIPPEINSSAGLESGSPELDEDYDLKSEDETEDDEDTIEEDEKHFTKRERQEELEALQNEVDLPVEELLRRYTSGRVSRETSPVKDENEDNLTSVSRVTSPVKDENQDNLASVGQDHGEDKNNLAASEETEGNPSVRRSNDSYGHLAISETHSHDLEPGMTTASVKSRKEDHTYDFNDEQEDVDFVLANGEEKDDEATLAVEEELAKADNEDHVEEIALLQKESEMPIEVLLARYKEDFGGKDISEDESESSFAVSEDSIVDSDENRQQADLDDDNVDLTECKLDPEPCSENVEGTFHEVAEDNDKDSSDKIADAAAAARSAQPTGFTYSTTKVRTKLPFLLKHSLREYQHIGLDWLVTMYEKKLNGILADEMGLGKTIMTIALLAHLACDKGIWGPHLIVVPTSVMLNWETEFLKWCPAFKILTYFGSAKERKLKRQGWMKLNSFHVCITTYRLVIQDSKMFKRKKWKYLILDEAHLIKNWKSQRWQTLLNFNSKRRILLTGTPLQNDLMELWSLMHFLMPHVFQSHQEFKDWFCNPIAGMVEGQEKINKEVIDRLHNVLRPFLLRRLKRDVEKQLPSKHEHVIFCRLSKRQRNLYEDFIASTETQATLTSGSFFGMISIIMQLRKVCNHPDLFEGRPIVSSFDMAGIDVQLSSTICSLLLESPFSKVDLEALGFLFTHLDFSMTSWEGDEIKAISTPSELIKQRVNLKDDLEAIPLSPKNRKNLQGTNIFEEIRKAVFEERIQESKDRAAAIAWWNSLRCQRKPTYSTSLRTLLTIKGPLDDLKANCSSYMYSSILADIVLSPIERFQKMIELVEAFTFAIPAARVPSPTCWCSKSDSPVFLSPSYKEKVTDLLSPLLSPIRPAIVRRQVYFPDRRLIQFDCGKLQELAMLLRKLKFGGHRALIFTQMTKMLDVLEAFINLYGYTYMRLDGSTPPEERQTLMQRFNTNPKIFLFILSTRSGGVGINLVGADTVIFYDSDWNPAMDQQAQDRCHRIGQTREVHIYRLISESTIEENILKKANQKRVLDNLVIQNGEYNTEFFKKLDPMELFSGHKALTTKDEKETSKHCGADIPLSNADVEAALKQAEDEADYMALKRVEQEEAVDNQEFTEEPVERPEDDELVNEDDIKADEPADQGLVAAGPAKEEMSLLHSDIRDERAVITTSSQEDDTDVLDDVKQMAAAAADAGQAISSFENQLRPIDRYAIRFLELWDPIIVEAAMENEAGFEEKEWELDHIEKYKEEMEAEIDDGEEPLVYEKWDADFATEAYRQQVEVLAQHQLMEDLENEAREREAAEVAEMVLTQNESAHVLKPKKKKKAKKAKYKSLKKGSLAAESKHVKSVVKIEDSTDDDNEEFGYVSSSDSDMVTPLSRMHMKGKKRDLIVDTDEEKTSKKKAKKHKKSLPNSDIKYKQTSALLDELEPSKPSDSMVVDNELKLTNRGKTVGKKFITSMPIKRVLMIKPEKLKKGNLWSRDCVPSPDSWLPQEDAILCAMVHEYGPNWNFVSGTLYGMTAGGAYRGRYRHPAYCCERYRELIQRHILSASDSAVNEKNLNTGSGKALLKVTEENIRTLLNVAAEQPDTEMLLQKHFSCLLSSIWRTSTRTGNDQMLSLNSPIFNRQFMGSVNHTQDLARKPWQGMKVTSLSRKLLESALQDSGPSQPDNTISRSRLQETQPINKLGLELTLEFPRGNDDSLNQFPPMISLSIDGSDSLNYVNEPPGEDVLKGSRVAAENRYRNAANACIEDSFGWASNTFPANDLKSRTGTKAQSLGKHKLSASDSAKSTKSKHRKLLAEQLEGAWVRPNDPNLKFDFTPGDREEEEEQEVDEKANSAEIEMISCSQWYDPFFTSGLDDCSLASDISEIE |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in ovules, but not in stamens.
Induction
Not regulated by vernalization.
Gene expression databases
Interaction
Subunit
Component of the SWR1 chromatin-remodeling complex composed of at least ARP6/ESD1/SUF3, PIE1, SWC6, SWC2 and H2AZs (HTA8, HTA9, HTA11). Interacts (via c-terminus) with SWC6 and ARP6 and (via N-terminus) with H2AZs.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q7X9V2 | ARP6 Q8LGE3 | 4 | EBI-1537462, EBI-1537316 | |
BINARY | Q7X9V2 | SWC6 Q9FHW2 | 4 | EBI-1537462, EBI-1537353 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, motif, domain, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-47 | Disordered | ||||
Sequence: MASKGGKSKPDIVMASKSGKSKPDNESRAKRQKTLEAPKEPRRPKTH | ||||||
Compositional bias | 21-47 | Basic and acidic residues | ||||
Sequence: SKPDNESRAKRQKTLEAPKEPRRPKTH | ||||||
Motif | 29-36 | Nuclear localization signal 1 | ||||
Sequence: AKRQKTLE | ||||||
Domain | 35-107 | HSA | ||||
Sequence: LEAPKEPRRPKTHWDHVLEEMAWLSKDFESERKWKLAQAKKVALRASKGMLDQASREERKLKEEEQRLRKVAL | ||||||
Coiled coil | 78-147 | |||||
Sequence: LRASKGMLDQASREERKLKEEEQRLRKVALNISKDMKKFWMKVEKLVLYKHQLVRNEKKKKAMDKQLEFL | ||||||
Region | 183-332 | Disordered | ||||
Sequence: KSDEERAEQIPPEINSSAGLESGSPELDEDYDLKSEDETEDDEDTIEEDEKHFTKRERQEELEALQNEVDLPVEELLRRYTSGRVSRETSPVKDENEDNLTSVSRVTSPVKDENQDNLASVGQDHGEDKNNLAASEETEGNPSVRRSNDS | ||||||
Compositional bias | 212-229 | Acidic residues | ||||
Sequence: DYDLKSEDETEDDEDTIE | ||||||
Coiled coil | 229-250 | |||||
Sequence: EEDEKHFTKRERQEELEALQNE | ||||||
Compositional bias | 230-247 | Basic and acidic residues | ||||
Sequence: EDEKHFTKRERQEELEAL | ||||||
Compositional bias | 254-282 | Basic and acidic residues | ||||
Sequence: PVEELLRRYTSGRVSRETSPVKDENEDNL | ||||||
Compositional bias | 283-303 | Polar residues | ||||
Sequence: TSVSRVTSPVKDENQDNLASV | ||||||
Compositional bias | 317-332 | Polar residues | ||||
Sequence: SEETEGNPSVRRSNDS | ||||||
Region | 340-359 | Disordered | ||||
Sequence: ETHSHDLEPGMTTASVKSRK | ||||||
Coiled coil | 392-416 | |||||
Sequence: EEELAKADNEDHVEEIALLQKESEM | ||||||
Region | 432-461 | Disordered | ||||
Sequence: KDISEDESESSFAVSEDSIVDSDENRQQAD | ||||||
Domain | 548-713 | Helicase ATP-binding | ||||
Sequence: VTMYEKKLNGILADEMGLGKTIMTIALLAHLACDKGIWGPHLIVVPTSVMLNWETEFLKWCPAFKILTYFGSAKERKLKRQGWMKLNSFHVCITTYRLVIQDSKMFKRKKWKYLILDEAHLIKNWKSQRWQTLLNFNSKRRILLTGTPLQNDLMELWSLMHFLMPH | ||||||
Motif | 664-667 | DEAH box | ||||
Sequence: DEAH | ||||||
Domain | 1076-1229 | Helicase C-terminal | ||||
Sequence: KLQELAMLLRKLKFGGHRALIFTQMTKMLDVLEAFINLYGYTYMRLDGSTPPEERQTLMQRFNTNPKIFLFILSTRSGGVGINLVGADTVIFYDSDWNPAMDQQAQDRCHRIGQTREVHIYRLISESTIEENILKKANQKRVLDNLVIQNGEYN | ||||||
Region | 1293-1313 | Disordered | ||||
Sequence: EEAVDNQEFTEEPVERPEDDE | ||||||
Compositional bias | 1297-1313 | Acidic residues | ||||
Sequence: DNQEFTEEPVERPEDDE | ||||||
Coiled coil | 1419-1492 | |||||
Sequence: FEEKEWELDHIEKYKEEMEAEIDDGEEPLVYEKWDADFATEAYRQQVEVLAQHQLMEDLENEAREREAAEVAEM | ||||||
Motif | 1506-1513 | Nuclear localization signal 2 | ||||
Sequence: KKKKKAKK | ||||||
Motif | 1570-1577 | Nuclear localization signal 3 | ||||
Sequence: KKRDLIVD | ||||||
Region | 1577-1597 | Disordered | ||||
Sequence: DTDEEKTSKKKAKKHKKSLPN | ||||||
Domain | 1673-1727 | Myb-like | ||||
Sequence: SWLPQEDAILCAMVHEYGPNWNFVSGTLYGMTAGGAYRGRYRHPAYCCERYRELI | ||||||
Region | 1843-1864 | Disordered | ||||
Sequence: ALQDSGPSQPDNTISRSRLQET | ||||||
Compositional bias | 1951-1968 | Polar residues | ||||
Sequence: KSRTGTKAQSLGKHKLSA | ||||||
Region | 1951-1977 | Disordered | ||||
Sequence: KSRTGTKAQSLGKHKLSASDSAKSTKS | ||||||
Coiled coil | 2006-2029 | |||||
Sequence: GDREEEEEQEVDEKANSAEIEMIS |
Sequence similarities
Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length2,055
- Mass (Da)234,048
- Last updated2003-10-01 v1
- ChecksumED38EC3C67D7F776
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1I9LQH7 | A0A1I9LQH7_ARATH | PIE1 | 2051 |
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 21-47 | Basic and acidic residues | ||||
Sequence: SKPDNESRAKRQKTLEAPKEPRRPKTH | ||||||
Compositional bias | 212-229 | Acidic residues | ||||
Sequence: DYDLKSEDETEDDEDTIE | ||||||
Compositional bias | 230-247 | Basic and acidic residues | ||||
Sequence: EDEKHFTKRERQEELEAL | ||||||
Compositional bias | 254-282 | Basic and acidic residues | ||||
Sequence: PVEELLRRYTSGRVSRETSPVKDENEDNL | ||||||
Compositional bias | 283-303 | Polar residues | ||||
Sequence: TSVSRVTSPVKDENQDNLASV | ||||||
Compositional bias | 317-332 | Polar residues | ||||
Sequence: SEETEGNPSVRRSNDS | ||||||
Compositional bias | 1297-1313 | Acidic residues | ||||
Sequence: DNQEFTEEPVERPEDDE | ||||||
Compositional bias | 1951-1968 | Polar residues | ||||
Sequence: KSRTGTKAQSLGKHKLSA |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY279398 EMBL· GenBank· DDBJ | AAP40633.1 EMBL· GenBank· DDBJ | mRNA | ||
AB024033 EMBL· GenBank· DDBJ | BAB02425.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002686 EMBL· GenBank· DDBJ | AEE75248.1 EMBL· GenBank· DDBJ | Genomic DNA |