Q7X8Z8 · GATA_ORYSJ
- ProteinGlutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial
- GeneGATA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids543 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
Miscellaneous
This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
Catalytic activity
- L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H+
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 123 | Charge relay system | ||||
Sequence: K | ||||||
Active site | 198 | Charge relay system | ||||
Sequence: S | ||||||
Active site | 222 | Acyl-ester intermediate | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast stroma | |
Cellular Component | glutamyl-tRNA(Gln) amidotransferase complex | |
Cellular Component | mitochondrion | |
Molecular Function | ATP binding | |
Molecular Function | glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity | |
Molecular Function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides | |
Biological Process | glutaminyl-tRNAGln biosynthesis via transamidation | |
Biological Process | mitochondrial translation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial
- EC number
- Short namesGlu-AdT subunit A
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ7X8Z8
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000413342 | 1-543 | UniProt | Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial | |||
Sequence: MPPPLQAQRLLLSHRRLPSPHLRCFTAVSSLPSAPAKTVAAAAAHAPSSILSIRESLLSGERTAAEITAEYLSRLRRTEPSVRSFIHVADAAAEREAEELDRRIATEGLDAVGPLAGVLVGVKDNLCTANMPSTGGSRILDGYQPAYDATAVRRLREAGAIVVGKTNLDEFGMGSTTEGSGFQVTTNPWDDSRVPGGSSGGSASAVSARQCVVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVVGCFGSSVVDTATILSVIAGHDKMDSTSSSHDVSDYKSELVPLDLLESKPLNGMRIGIIQETLGEGVETGVISSIKDAASHLEQLGSVVEEVSLPSFSLGLPAYYILASSEASSNLSRYDGIRYGRQVSGDDLNELYGGSRANGLGHEVKMRILMGTYALSAGYYDAYYKRAQQVRTLVKKSFKEALERYDILVSPAAPSAAYKIGEKINDPLAMYAGDTMTVNVNLAGLPALVVPCGFVEGGSAGLPVGLQMIGSPFSEGNLLRIGHIFEQTLQNYSFVPPLLAES | |||||||
Modified residue (large scale data) | 385 | PTMeXchange | Phosphoserine | ||||
Sequence: S |
Proteomic databases
Interaction
Subunit
Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A, B and C subunits.
Protein-protein interaction databases
Structure
Family & Domains
Sequence similarities
Belongs to the amidase family. GatA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length543
- Mass (Da)57,219
- Last updated2011-10-19 v3
- Checksum0AF76FA9D1EB6484
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0N7KJT1 | A0A0N7KJT1_ORYSJ | GATA | 534 |
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL606454 EMBL· GenBank· DDBJ | CAD41903.2 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AL731607 EMBL· GenBank· DDBJ | CAE05977.2 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AP008210 EMBL· GenBank· DDBJ | BAF15963.2 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AP014960 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CM000141 EMBL· GenBank· DDBJ | EAZ32175.1 EMBL· GenBank· DDBJ | Genomic DNA |