Q7X7S8 · COLD1_ORYSJ
- ProteinGPCR-type G protein COLD1
- GeneCOLD1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids468 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in chilling tolerance. Interacts with the G-protein alpha subunit GPA1 to activate the calcium channel for sensing low temperature and to accelerate G-protein GTPase activity.
Miscellaneous
In cold sensitive indica cultivars (AC A2XX57) Lys-187 is replaced by Met-187 or Thr-187. These polymorphisms are associated with divergence in chilling tolerance of rice cultivars. COLD1 confers adaptation of japonica rice to chilling and originated from the Chinese wild populations of Oryza rufipogon.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | plasma membrane | |
Molecular Function | abscisic acid binding | |
Molecular Function | GTPase binding | |
Biological Process | cellular response to cold | |
Biological Process | response to abscisic acid |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameGPCR-type G protein COLD1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ7X7S8
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 7-27 | Helical; Name=1 | ||||
Sequence: VYGGGVVGASLVGLGWAGLWF | ||||||
Transmembrane | 45-65 | Helical; Name=2 | ||||
Sequence: ILFGLVFAFSCNLFQLVLFEI | ||||||
Transmembrane | 82-102 | Helical; Name=3 | ||||
Sequence: LFCLILLLVFVLPYYHCYLLL | ||||||
Transmembrane | 112-132 | Helical; Name=4 | ||||
Sequence: ALLVAALFLLVFLYGFWRMGI | ||||||
Transmembrane | 153-173 | Helical; Name=5 | ||||
Sequence: IGVIGVSVMAVLSGFGAVNLP | ||||||
Transmembrane | 297-319 | Helical; Name=6 | ||||
Sequence: LLGYALSVYCVYKMLKSLQSVVF | ||||||
Transmembrane | 345-365 | Helical; Name=7 | ||||
Sequence: VTLLSQYISLIFIGMLVVISV | ||||||
Transmembrane | 389-409 | Helical; Name=8 | ||||
Sequence: NVVLFLSEIMGMYFISSILLI | ||||||
Transmembrane | 437-457 | Helical; Name=9 | ||||
Sequence: WFDAIFVASAFLSLLLISAQY |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
No visible phenotype under normal growth conditions, but mutant plants are sensitive to cold.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 2 | No effect on cellular localization. | ||||
Sequence: G → A |
PTM/Processing
Features
Showing features for initiator methionine, lipidation, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Lipidation | 2 | N-myristoyl glycine | ||||
Sequence: G | ||||||
Chain | PRO_0000432809 | 2-468 | GPCR-type G protein COLD1 | |||
Sequence: GWGAVVYGGGVVGASLVGLGWAGLWFLNRRLYKEYEERRALVQILFGLVFAFSCNLFQLVLFEILPVLSKHARFLNWHLDLFCLILLLVFVLPYYHCYLLLRNSGVRRERALLVAALFLLVFLYGFWRMGIHFPMPSPEKGFFTMPQLVSRIGVIGVSVMAVLSGFGAVNLPYSYLSLFIREIDEKDIKTLERQLMQSMETCIAKKKKIVLSKMEMERIQGSEEKLKARSFLKRIVGTVVRSVQEDQTEQDIKSLDAEVQALEELSKQLFLEIYELRQAKIAAAFSRTWRGHAQNLLGYALSVYCVYKMLKSLQSVVFKEAGSVDPVTMTITIFLRHFDIGIDVTLLSQYISLIFIGMLVVISVRGFLANVMKFFFAVSRVGSGSTTNVVLFLSEIMGMYFISSILLIRKSLANEYRVIITDVLGGDIQFDFYHRWFDAIFVASAFLSLLLISAQYTSRQTDKHPID |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Structure
Family & Domains
Features
Showing features for coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Coiled coil | 243-279 | |||||
Sequence: SVQEDQTEQDIKSLDAEVQALEELSKQLFLEIYELRQ |
Sequence similarities
Belongs to the Golgi pH regulator (TC 1.A.38) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length468
- Mass (Da)53,332
- Last updated2004-07-05 v2
- Checksum5205D49B2E0530D3
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 9 | in Ref. 6; AK318606 and 5; EAZ31870 | ||||
Sequence: G → E |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL606683 EMBL· GenBank· DDBJ | CAD41818.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP008210 EMBL· GenBank· DDBJ | BAF15676.2 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AP014960 EMBL· GenBank· DDBJ | BAS90836.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CM000141 EMBL· GenBank· DDBJ | EAZ31870.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK318606 EMBL· GenBank· DDBJ | - | mRNA | No translation available. |