Q7WUL3 · NAG3_CELFI
- ProteinBeta-N-acetylglucosaminidase/beta-glucosidase
- Genenag3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids564 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Catalyzes the cleavage of beta-N-acetyl-D-glucosaminides and beta-D-glucosides. Might be involved in the degradation of glucuronic acid-containing glycosaminoglycans such as hyaluronic acid.
Miscellaneous
Catalyzes hydrolysis by a double-displacement mechanism via a covalent glycosyl-enzyme intermediate, involving the participation of a catalytic nucleophilic group in the enzyme active site.
Catalytic activity
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
2.7 mM | 4'-nitrophenyl beta-N-acetyl-D-glucosaminide |
The catalytic efficiencies against 4'-nitrophenyl beta-N-acetyl-D-glucosaminide and 4'-nitrophenyl beta-D-glucopyranoside are similar.
pH Dependence
Optimum pH is 7.3 with 4'-nitrophenyl beta-D-glucopyranoside as substrate. Precipitates below pH 6 and stable from pH 6.8 to 8.4.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 283 | Nucleophile | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | beta-glucosidase activity | |
Molecular Function | beta-N-acetylhexosaminidase activity | |
Molecular Function | N-acetyl-beta-D-galactosaminidase activity | |
Molecular Function | scopolin beta-glucosidase activity | |
Biological Process | peptidoglycan turnover | |
Biological Process | polysaccharide catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameBeta-N-acetylglucosaminidase/beta-glucosidase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Micrococcales > Cellulomonadaceae > Cellulomonas
Accessions
- Primary accessionQ7WUL3
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000252454 | 1-564 | Beta-N-acetylglucosaminidase/beta-glucosidase | |||
Sequence: MIDLTAAPFSLDDDGIAWVRTTLAEMGEDEKLGQLFCLITYTSDPEYLGYLTRGLHVGGVMLRTMTAADAAATVTTLQSTATVPLLISANLEGGASQTVQEATHVGSNMALAATGSTDHVRRAATVIGREARALGINWAFTPVVDIDLNFRNPITNTRTFGADAATVAAMGAEYVEAIQAQGLAASAKHFPGDGVDERDQHLLASVNTMSVEEWDDSFGVVYRAAIAAGVKTVMVGHIMLPAYSRALRPGVADRDILPGVVAEELLNDLLRDRLGFNGLVVSDSTTMAGLASVLPRSQAVPRVIAAGCDMFLFTKNLDEDFGYMRAGIRDGVITPERLDEAVTRILALKASLGLHRGTNLPAQGAAGVLADPDHSATAREVAASSITLVKEEPGVLPITRERYPRVLVYDLQNGGSPIGQGARAGAVEQFVDALVEAGHDVTRFEPGGGWEGMAAPTTDVTERHDLVLYLANLSTRSNQTVVRIEWAEPMGANVPAYVHSVPTVFVSFENPYHLFDVPRVRTLINTYGSSPVVLETLLAALQGKAPFAGSSPVDAFCGQWDTHL |
Structure
Sequence
- Sequence statusComplete
- Length564
- Mass (Da)59,993
- Last updated2003-10-01 v1
- Checksum9B1428A4561F71D3
Keywords
- Technical term