Q7VP66 · CARA_HAEDU

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site50L-glutamine (UniProtKB | ChEBI)
Binding site251L-glutamine (UniProtKB | ChEBI)
Binding site253L-glutamine (UniProtKB | ChEBI)
Active site279Nucleophile
Binding site280L-glutamine (UniProtKB | ChEBI)
Binding site283L-glutamine (UniProtKB | ChEBI)
Binding site321L-glutamine (UniProtKB | ChEBI)
Binding site323L-glutamine (UniProtKB | ChEBI)
Binding site324L-glutamine (UniProtKB | ChEBI)
Active site363
Active site365

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • Ordered locus names
      HD_0235

Organism names

Accessions

  • Primary accession
    Q7VP66

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001122791-389Carbamoyl phosphate synthase small chain

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-199CPSase
Domain203-389Glutamine amidotransferase type-1

Sequence similarities

Belongs to the CarA family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    389
  • Mass (Da)
    42,748
  • Last updated
    2003-10-01 v1
  • Checksum
    7553218FAB9F38C6
MFNPAILVLADGSTFYGKSIGYQAVSAYSIGEVVFNTAMTGYQEILTDPSYAKQIVTLTYPHIGNTGTNDEDNESNQIYASGLIIRDLPLLHSNFRATSSLSDYLIQHNIIAIADIDTRRLTRILRETGSQAGCIYIPNENESLAQAVEKAQDLAKSFGSMAGKDLAKEVTCQHRYEWQEGIWHLPTNNAGKPFNLQTTPTFHVVAYDFGIKQNILRMLAERGCKITVVPAQTSAEDILALNPDGIFLSNGPGDPEPCTYAIQSIRQLLNTQKPIFGICLGHQLLGLAVGAKTRKMAFGHHGANHPVQDLKTEHVLITSQNHGFEIDEYSLPENVIVTHRSLFDNSVQGIELTNQVAFSFQGHPEANPGPQDVAYLFDKFINAVQATKA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE017143
EMBL· GenBank· DDBJ
AAP95221.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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