Q7V042 · Q7V042_PROMP
- ProteinGlutamine-dependent NAD(+) synthetase
- GenenadE
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids565 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.
Catalytic activity
- deamido-NAD+ + L-glutamine + ATP + H2O = L-glutamate + AMP + diphosphate + NAD+ + H+
Pathway
Cofactor biosynthesis; NAD+ biosynthesis; NAD+ from deamido-NAD+ (L-Gln route): step 1/1.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 41 | Proton acceptor; for glutaminase activity | |||
Active site | 116 | For glutaminase activity | |||
Binding site | 122 | L-glutamine (UniProtKB | ChEBI) | |||
Active site | 156 | Nucleophile; for glutaminase activity | |||
Binding site | 193 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 199 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 302-309 | ATP (UniProtKB | ChEBI) | |||
Binding site | 385 | deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits | |||
Binding site | 409 | ATP (UniProtKB | ChEBI) | |||
Binding site | 414 | deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits | |||
Binding site | 538 | deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | glutaminase activity | |
Molecular Function | NAD+ synthase (glutamine-hydrolyzing) activity | |
Molecular Function | NAD+ synthase activity | |
Biological Process | NAD biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamine-dependent NAD(+) synthetase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Synechococcales > Prochlorococcaceae > Prochlorococcus
Accessions
- Primary accessionQ7V042
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-263 | CN hydrolase | |||
Sequence similarities
Belongs to the NAD synthetase family.
In the C-terminal section; belongs to the NAD synthetase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length565
- Mass (Da)63,319
- Last updated2003-10-01 v1
- Checksum4D1C6B8EA4F22B71
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX548174 EMBL· GenBank· DDBJ | CAE19905.1 EMBL· GenBank· DDBJ | Genomic DNA |