Q7V042 · Q7V042_PROMP

Function

function

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Cofactor biosynthesis; NAD+ biosynthesis; NAD+ from deamido-NAD+ (L-Gln route): step 1/1.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site41Proton acceptor; for glutaminase activity
Active site116For glutaminase activity
Binding site122L-glutamine (UniProtKB | ChEBI)
Active site156Nucleophile; for glutaminase activity
Binding site193L-glutamine (UniProtKB | ChEBI)
Binding site199L-glutamine (UniProtKB | ChEBI)
Binding site302-309ATP (UniProtKB | ChEBI)
Binding site385deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site409ATP (UniProtKB | ChEBI)
Binding site414deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site538deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionglutaminase activity
Molecular FunctionNAD+ synthase (glutamine-hydrolyzing) activity
Molecular FunctionNAD+ synthase activity
Biological ProcessNAD biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glutamine-dependent NAD(+) synthetase
  • EC number
  • Alternative names
    • NAD(+) synthase [glutamine-hydrolyzing]

Gene names

    • Name
      nadE
    • Ordered locus names
      PMM1446

Organism names

Accessions

  • Primary accession
    Q7V042

Proteomes

Subcellular Location

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-263CN hydrolase

Sequence similarities

Belongs to the NAD synthetase family.
In the C-terminal section; belongs to the NAD synthetase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    565
  • Mass (Da)
    63,319
  • Last updated
    2003-10-01 v1
  • Checksum
    4D1C6B8EA4F22B71
MNFFIAQLNPIVGDLEGNAKKILTETGKAYSNCADMVLTPELSLWGYPAKDLLLKKNLIERQYSILDQLSISIFKKYGNLSVAVGIAEKIDDSFFPNLYNSIVLIERGKWKTIARKIILPTYEVFDEKRYFRSEQRVSVISKTIKNKTYKLGITICEDLWVNENIEGRGIHKKNPIIDLKSKKIDLLLNLSASPYTFKKFNLRNKISSFAAKYLQVPLIYVNQIGANDDLIFDGNSFIMNKNGSKIKQLKSFSEDTSSWDINENLNLVDEKISSEISSVFDALVLGVKDYAKKCGFRSALIGLSGGIDSALVSVIATAALGSEKIFCVSMPSKWSSEHSKIDAKDLVARLNINLNTISIENIMSSFEKSFIESLNFKTEGITNQNIQSRIRGTLLMALANQENHLLLSTGNKSELAVGYCTLYGDMNGGLSVIGDLYKTNVFKLCRWLDSKDSIEHRKAYKLDTKVKIIGDQICNKPPSAELGPDQLDTDSLPPYSLLDKILKGIIEEKKDLQLLEQDGHTKEQILKIITLIKKAEFKRKQAPPILKLSSQSLGSDWRVPIAISN

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BX548174
EMBL· GenBank· DDBJ
CAE19905.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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