Q7UMW2 · CYSNC_RHOBA
- ProteinBifunctional enzyme CysN/CysC
- GenecysNC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids647 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway. APS synthesis involves the formation of a high-energy phosphoric-sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.
APS kinase catalyzes the synthesis of activated sulfate.
Catalytic activity
- ATP + H+ + sulfate = adenosine 5'-phosphosulfate + diphosphate
Pathway
Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.
Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | adenylylsulfate kinase activity | |
Molecular Function | ATP binding | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | sulfate adenylyltransferase (ATP) activity | |
Biological Process | hydrogen sulfide biosynthetic process | |
Biological Process | sulfate assimilation | |
Biological Process | sulfur compound metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBifunctional enzyme CysN/CysC
Including 2 domains:
- Recommended nameSulfate adenylyltransferase subunit 1
- EC number
- Alternative names
- Recommended nameAdenylyl-sulfate kinase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Planctomycetota > Planctomycetia > Pirellulales > Pirellulaceae > Rhodopirellula
Accessions
- Primary accessionQ7UMW2
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000239080 | 1-647 | Bifunctional enzyme CysN/CysC | |||
Sequence: MSHQSDLIATDIDAYLKQHEQKQLLRFITCGSVDDGKSTLIGRLLYDSKLVYEDELAKVQSDSVRQGSVAGGFDPSLFMDGLKEEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRNMATGASSADLAIILIDARHGVLTQTRRHSFIVSLLGIRHVVVAVNKMDIDGVDYSEDRFNEICDDYRSFATRLDLPDLHFIPISALNGDNLVDRSENMPWYTGSTLMNFLETVYIGSDRNLQDFRLPVQLVNRPNLNFRGFCGTIASGIIRKGEEITVLPSRQKSKVKEIVTYDGNLDEAYAPLAVTLTLEDEIDASRGDMIVRSGNLPRSESDVEAMLVWMNEEAMVPGKTYLVKHSTQTVPGNVETLAYKVDVNDLHRMPAPTLELNEIGRVRLSLSAPIHHDPYRRNRTTGAIILVDRITNATVAAGMILDRGTTGSHKSVWDDEVSTDDSSDALSTVTTEERAARFGQKPATVLLTGLTGSGKTSIARAVERKLFDAGRSVAVVDGEFVRRGLSRDLGFSADDRSENLRRSGHLAHTLNDAGLICLASLVAPSDDVRQKVGKLIGEDQFLVVHVATPLDVCRERDTKGQYAKADAGELSNFPGVTAKYDVPTNPDLAVDASTTSIAECADAVVELLKLKGFIK |
Interaction
Subunit
Heterodimer composed of CysD, the smaller subunit, and CysNC.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-472 | Sulfate adenylyltransferase | ||||
Sequence: MSHQSDLIATDIDAYLKQHEQKQLLRFITCGSVDDGKSTLIGRLLYDSKLVYEDELAKVQSDSVRQGSVAGGFDPSLFMDGLKEEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRNMATGASSADLAIILIDARHGVLTQTRRHSFIVSLLGIRHVVVAVNKMDIDGVDYSEDRFNEICDDYRSFATRLDLPDLHFIPISALNGDNLVDRSENMPWYTGSTLMNFLETVYIGSDRNLQDFRLPVQLVNRPNLNFRGFCGTIASGIIRKGEEITVLPSRQKSKVKEIVTYDGNLDEAYAPLAVTLTLEDEIDASRGDMIVRSGNLPRSESDVEAMLVWMNEEAMVPGKTYLVKHSTQTVPGNVETLAYKVDVNDLHRMPAPTLELNEIGRVRLSLSAPIHHDPYRRNRTTGAIILVDRITNATVAAGMILDRGTTGSHKSVWDDEVSTDDSSDALSTVTTEERAARFGQ | ||||||
Domain | 22-239 | tr-type G | ||||
Sequence: KQLLRFITCGSVDDGKSTLIGRLLYDSKLVYEDELAKVQSDSVRQGSVAGGFDPSLFMDGLKEEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRNMATGASSADLAIILIDARHGVLTQTRRHSFIVSLLGIRHVVVAVNKMDIDGVDYSEDRFNEICDDYRSFATRLDLPDLHFIPISALNGDNLVDRSENMPWYTGSTLMNFLETVYIGSDR | ||||||
Region | 31-38 | G1 | ||||
Sequence: GSVDDGKS | ||||||
Region | 89-93 | G2 | ||||
Sequence: GITID | ||||||
Region | 110-113 | G3 | ||||
Sequence: DTPG | ||||||
Region | 165-168 | G4 | ||||
Sequence: NKMD | ||||||
Region | 204-206 | G5 | ||||
Sequence: SAL | ||||||
Region | 473-614 | Adenylyl-sulfate kinase | ||||
Sequence: KPATVLLTGLTGSGKTSIARAVERKLFDAGRSVAVVDGEFVRRGLSRDLGFSADDRSENLRRSGHLAHTLNDAGLICLASLVAPSDDVRQKVGKLIGEDQFLVVHVATPLDVCRERDTKGQYAKADAGELSNFPGVTAKYDV |
Sequence similarities
In the C-terminal section; belongs to the APS kinase family.
In the N-terminal section; belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length647
- Mass (Da)71,170
- Last updated2003-10-01 v1
- Checksum59113B3A36831A31
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX294146 EMBL· GenBank· DDBJ | CAD75662.1 EMBL· GenBank· DDBJ | Genomic DNA |