Q7UJ52 · SYT_RHOBA
- ProteinThreonine--tRNA ligase
- GenethrS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids743 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).
Catalytic activity
- tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H+
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 416 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 467 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 596 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | threonine-tRNA ligase activity | |
Molecular Function | tRNA binding | |
Biological Process | threonyl-tRNA aminoacylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThreonine--tRNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Planctomycetota > Planctomycetia > Pirellulales > Pirellulaceae > Rhodopirellula
Accessions
- Primary accessionQ7UJ52
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000101034 | 1-743 | Threonine--tRNA ligase | ||
Interaction
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-15 | Polar residues | |||
Region | 1-30 | Disordered | |||
Domain | 13-76 | TGS | |||
Region | 264-619 | Catalytic | |||
Region | 354-404 | Insert | |||
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length743
- Mass (Da)83,078
- Last updated2006-02-07 v2
- MD5 Checksum1941FB0D2CFA193F29AE1DDE82CF49B9
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-15 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX294154 EMBL· GenBank· DDBJ | CAD77406.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation |