Q7U4Z8 · GSMT_PARMW
- ProteinGlycine/sarcosine N-methyltransferase
- GenebsmA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids282 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the methylation of glycine and sarcosine to sarcosine and dimethylglycine, respectively, with S-adenosylmethionine (AdoMet) acting as the methyl donor. It has strict specificity for glycine and sarcosine as the methyl group acceptors.
Catalytic activity
- glycine + 2 S-adenosyl-L-methionine = 2 H+ + N,N-dimethylglycine + 2 S-adenosyl-L-homocysteineThis reaction proceeds in the forward direction.
- glycine + S-adenosyl-L-methionine = H+ + S-adenosyl-L-homocysteine + sarcosineThis reaction proceeds in the forward direction.
- S-adenosyl-L-methionine + sarcosine = H+ + N,N-dimethylglycine + S-adenosyl-L-homocysteineThis reaction proceeds in the forward direction.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.3 mM | AdoMet | 8.2 | 37 | with glycine | ||
0.44 mM | AdoMet | 8.0 | 37 | with sarcosine | ||
2.94 mM | sarcosine | 8.0 | 37 | |||
5.22 mM | glycine | 8.2 | 37 |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
0.74 μmol/min/mg | 8.0 | 37 | with AdoMet as substrate, with sarcosine | ||
0.78 μmol/min/mg | 8.0 | 37 | with sarcosine as substrate | ||
1.3 μmol/min/mg | 8.2 | 37 | with AdoMet as substrate, with glycine | ||
1.48 μmol/min/mg | 8.2 | 37 | with glycine as substrate |
pH Dependence
Optimum pH is around 8.2 and 8.0 for glycine and sarcosine, respectively.
Pathway
Amine and polyamine biosynthesis; betaine biosynthesis via glycine pathway; betaine from glycine: step 1/3.
Amine and polyamine biosynthesis; betaine biosynthesis via glycine pathway; betaine from glycine: step 2/3.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 35 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 43 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Binding site | 52 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 76 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 97 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 123-124 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DW | ||||||
Binding site | 141 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 143 | substrate | ||||
Sequence: N | ||||||
Binding site | 176 | substrate | ||||
Sequence: R | ||||||
Binding site | 217 | substrate | ||||
Sequence: Y |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | glycine binding | |
Molecular Function | glycine N-methyltransferase activity | |
Molecular Function | identical protein binding | |
Molecular Function | S-adenosyl-L-methionine binding | |
Molecular Function | sarcosine N-methyltransferase activity | |
Biological Process | glycine betaine biosynthetic process from glycine | |
Biological Process | methylation | |
Biological Process | one-carbon metabolic process | |
Biological Process | protein homotetramerization | |
Biological Process | regulation of gluconeogenesis | |
Biological Process | S-adenosylhomocysteine metabolic process | |
Biological Process | S-adenosylmethionine metabolic process | |
Biological Process | sarcosine metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlycine/sarcosine N-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Synechococcales > Prochlorococcaceae > Parasynechococcus > Parasynechococcus marenigrum
Accessions
- Primary accessionQ7U4Z8
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000413612 | 1-282 | Glycine/sarcosine N-methyltransferase | |||
Sequence: MTSTQNHPLQTQDDQQRFGQSPESVRETDHYQQEYIEDFTDRWDRLIDWNARAEAEGDFFIRLLKEHGARSVLDVATGTGFHSIRLLEEGFDVVSADGSPNMLARAFRNARNRDQLLRTSQADWRFLNRDIHGEFDAVICLGNSFTHLFKERDRRKALAEYYAVLKHNGILILDHRNYDRLLEGGSAVRQGKGNVYCGKDVEVGPEHVDEGLARFRYSFSDGGVYHLNMFPLRYGYVRRLMSEVGFQQITSFGDYQRDFENPDFYVHVAEKEYRFDVDTTMH |
Interaction
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-22 | Polar residues | ||||
Sequence: MTSTQNHPLQTQDDQQRFGQSP | ||||||
Region | 1-27 | Disordered | ||||
Sequence: MTSTQNHPLQTQDDQQRFGQSPESVRE |
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length282
- Mass (Da)32,894
- Last updated2003-10-01 v1
- Checksum0A3235C5445874DC
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-22 | Polar residues | ||||
Sequence: MTSTQNHPLQTQDDQQRFGQSP |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX569694 EMBL· GenBank· DDBJ | CAE08429.1 EMBL· GenBank· DDBJ | Genomic DNA |