Q7TNN9 · RHCG_RAT
- ProteinAmmonium transporter Rh type C
- GeneRhcg
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids497 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Ammonium transporter involved in the maintenance of acid-base homeostasis. Transports ammonium and its related derivative methylammonium across the plasma membrane of epithelial cells likely contributing to renal transepithelial ammonia transport and ammonia metabolism. Postulated to primarily mediate an electroneutral bidirectional transport of NH3 ammonia species according to a mechanism that implies interaction of an NH4+ ion with acidic residues of the pore entry followed by dissociation of NH4+ into NH3 and H+. As a result NH3 transits through the central pore and is protonated on the extracellular side reforming NH4+ (By similarity).
May act as a CO2 channel providing for renal acid secretion (By similarity).
May act as a CO2 channel providing for renal acid secretion (By similarity).
Catalytic activity
- NH4+(in) = NH4+(out)This reaction proceeds in the forward and the backward directions.
- methylamine(out) = methylamine(in)This reaction proceeds in the forward direction.
- CO2(out) = CO2(in)This reaction proceeds in the forward direction.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | apical plasma membrane | |
Cellular Component | basal plasma membrane | |
Cellular Component | basolateral plasma membrane | |
Cellular Component | cytoplasmic vesicle | |
Cellular Component | plasma membrane | |
Molecular Function | ammonium channel activity | |
Molecular Function | ankyrin binding | |
Molecular Function | carbon dioxide transmembrane transporter activity | |
Molecular Function | identical protein binding | |
Biological Process | ammonium homeostasis | |
Biological Process | ammonium transmembrane transport | |
Biological Process | intracellular monoatomic ion homeostasis | |
Biological Process | regulation of pH | |
Biological Process | transepithelial ammonium transport |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAmmonium transporter Rh type C
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ7TNN9
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Apical cell membrane ; Multi-pass membrane protein
Note: Also detected at the basolateral membrane and in subapical vesicles. Chronic acidosis induces relocalization to the apical cell membrane.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-9 | Cytoplasmic | ||||
Sequence: MAWNTNLRG | ||||||
Transmembrane | 10-30 | Helical | ||||
Sequence: RLPITCLILQVTMVVLFGVFV | ||||||
Topological domain | 31-61 | Extracellular | ||||
Sequence: RYDIQADAHWWLEKKRKNISSDVENEFYYRY | ||||||
Transmembrane | 62-82 | Helical | ||||
Sequence: PSFEDVHAMVFVGFGFLMTYL | ||||||
Topological domain | 83-93 | Cytoplasmic | ||||
Sequence: QRYGFSAVGFN | ||||||
Transmembrane | 94-114 | Helical | ||||
Sequence: FLLAAFGIQWALLMQGWFHFF | ||||||
Topological domain | 115-125 | Extracellular | ||||
Sequence: EEGHILLSVEN | ||||||
Transmembrane | 126-145 | Helical | ||||
Sequence: LIQADFCVASTCVAFGAVLG | ||||||
Topological domain | 146-151 | Cytoplasmic | ||||
Sequence: KISPMQ | ||||||
Transmembrane | 152-174 | Helical | ||||
Sequence: LLIMTFFQVTLFTVNEFILLNLI | ||||||
Topological domain | 175-179 | Extracellular | ||||
Sequence: EAKDA | ||||||
Transmembrane | 180-200 | Helical | ||||
Sequence: GGSMTIHTFGAYFGLTVTWIL | ||||||
Topological domain | 201-219 | Cytoplasmic | ||||
Sequence: YRKNLEQSKQRQSSVYHSD | ||||||
Transmembrane | 220-240 | Helical | ||||
Sequence: LFAMIGTLFLWIYWPSFNSAS | ||||||
Topological domain | 241-251 | Extracellular | ||||
Sequence: SFHGDTQHRAA | ||||||
Transmembrane | 252-272 | Helical | ||||
Sequence: LNTYLSLAASVLTTVAVSSVI | ||||||
Topological domain | 273-282 | Cytoplasmic | ||||
Sequence: HKKGKLDMVH | ||||||
Transmembrane | 283-303 | Helical | ||||
Sequence: IQNATLAGGVGVGTAAEMMLT | ||||||
Topological domain | 304 | Extracellular | ||||
Sequence: P | ||||||
Transmembrane | 305-325 | Helical | ||||
Sequence: YGALIVGFFCGILSTLGFAYL | ||||||
Topological domain | 326-340 | Cytoplasmic | ||||
Sequence: SPFLESRLRIQDTCG | ||||||
Transmembrane | 341-361 | Helical | ||||
Sequence: IHNLHGIPGIIGGIVGAVTAA | ||||||
Topological domain | 362-395 | Extracellular | ||||
Sequence: YSSPDVYGEPGIVHSFGFGGYKADWTKRMQGRSQ | ||||||
Transmembrane | 396-416 | Helical | ||||
Sequence: IFGLLLSLAMALVGGIIVGFI | ||||||
Topological domain | 417-497 | Cytoplasmic | ||||
Sequence: LKLPFWGQASDENCFEDAIYWEVPEEVNTVYIPEDLAHKHSTSLVPAIPLVLSTPSASIVPPVPPTPPASLATVTSSSLVH |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000283584 | 1-497 | Ammonium transporter Rh type C | |||
Sequence: MAWNTNLRGRLPITCLILQVTMVVLFGVFVRYDIQADAHWWLEKKRKNISSDVENEFYYRYPSFEDVHAMVFVGFGFLMTYLQRYGFSAVGFNFLLAAFGIQWALLMQGWFHFFEEGHILLSVENLIQADFCVASTCVAFGAVLGKISPMQLLIMTFFQVTLFTVNEFILLNLIEAKDAGGSMTIHTFGAYFGLTVTWILYRKNLEQSKQRQSSVYHSDLFAMIGTLFLWIYWPSFNSASSFHGDTQHRAALNTYLSLAASVLTTVAVSSVIHKKGKLDMVHIQNATLAGGVGVGTAAEMMLTPYGALIVGFFCGILSTLGFAYLSPFLESRLRIQDTCGIHNLHGIPGIIGGIVGAVTAAYSSPDVYGEPGIVHSFGFGGYKADWTKRMQGRSQIFGLLLSLAMALVGGIIVGFILKLPFWGQASDENCFEDAIYWEVPEEVNTVYIPEDLAHKHSTSLVPAIPLVLSTPSASIVPPVPPTPPASLATVTSSSLVH | ||||||
Glycosylation | 48 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Post-translational modification
N-glycosylated.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed by connecting tubule cells and intercalated cells of the collecting duct in kidney (at protein level).
Interaction
Structure
Family & Domains
Sequence similarities
Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length497
- Mass (Da)54,731
- Last updated2003-10-01 v1
- ChecksumC696D975833F3B67
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I5Y844 | A0A8I5Y844_RAT | Rhcg | 464 | ||
F1M794 | F1M794_RAT | Rhcg | 520 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY129073 EMBL· GenBank· DDBJ | AAN07791.1 EMBL· GenBank· DDBJ | mRNA |