Q7TN73 · CASD1_MOUSE

  • Protein
    N-acetylneuraminate (7)9-O-acetyltransferase
  • Gene
    Casd1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    4/5

Function

function

Key enzyme in the biosynthesis of O-acetylated (O-Ac) sialoglycans such as gangliosides O-AcGD3 and O-AcGD2, which affects various processes such as cell-cell interactions, host-pathogen recognition. Catalyzes the transfer of an acetyl group from a donor, the acetyl-coenzyme-A molecule (acetyl-CoA), to the C7/8/9 OH-position of a glycosidically bound sialic acid residue. The primary site of O-acetyl group transfer on sialic acid is thought to be C7, from which the O-acetyl group could subsequently migrate under acidic pH conditions to the C8 and to the C9 positions, which are more stable positions. Can also transfer the acetyl group from acetyl-CoA to free sialate (N-acetylneuraminate, Neu5Ac) in vitro, but has preferred substrate specificity for CMP-activated sialate (CMP-Neu5Ac), which can initially be acetylated at C7 and non-enzymatically migrate to C9, resulting in the formation of 9-O-acetylated CMP-Neu5Ac (CMP-Neu5,9Ac2). CMP-Neu5,9Ac2 may be used by sialyltransferases as a sialate donor for glycoconjugate acceptors. O-acetylation at position C9 of ganglioside GD3 can counteract the pro-apoptotic effects of the ganglioside GD3 in tumor cells.

Miscellaneous

The Casd1 locus is imprinted. Maternal inherited gene is expressed, while the paternal inherited gene is silenced.

Catalytic activity

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site94Acyl-ester intermediate
Active site270
Active site273

GO annotations

AspectTerm
Cellular ComponentGolgi membrane
Molecular FunctionN-acetylneuraminate 7-O(or 9-O)-acetyltransferase activity
Biological Processcarbohydrate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    N-acetylneuraminate (7)9-O-acetyltransferase
  • EC number
  • Alternative names
    • CAS1 domain-containing protein 1
    • CAS1 protein
      (Cas1p
      )
    • Sialate O-acetyltransferase
      (SOAT
      )

Gene names

    • Name
      Casd1
    • Synonyms
      Cas1, Cast1

Organism names

  • Taxonomic identifier
  • Strains
    • 129
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q7TN73
  • Secondary accessions
    • Q1RN01
    • Q6PD39
    • Q8VEF5

Proteomes

Organism-specific databases

Subcellular Location

Golgi apparatus membrane
; Multi-pass membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-18Cytoplasmic
Transmembrane19-39Helical
Topological domain40-313Lumenal
Transmembrane314-334Helical
Topological domain335-363Cytoplasmic
Transmembrane364-384Helical
Topological domain385-395Lumenal
Transmembrane396-416Helical
Topological domain417-439Cytoplasmic
Transmembrane440-460Helical
Topological domain461Lumenal
Transmembrane462-482Helical
Topological domain483-486Cytoplasmic
Transmembrane487-507Helical
Topological domain508-513Lumenal
Transmembrane514-534Helical
Topological domain535-546Cytoplasmic
Transmembrane547-567Helical
Topological domain568-599Lumenal
Transmembrane600-620Helical
Topological domain621-638Cytoplasmic
Transmembrane639-659Helical
Topological domain660-671Lumenal
Transmembrane672-692Helical
Topological domain693-698Cytoplasmic
Transmembrane699-719Helical
Topological domain720-725Lumenal
Transmembrane726-746Helical
Topological domain747-770Cytoplasmic
Transmembrane771-791Helical
Topological domain792-797Lumenal

Keywords

PTM/Processing

Features

Showing features for chain, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00003072311-797N-acetylneuraminate 79-O-acetyltransferase
Glycosylation46N-linked (GlcNAc...) asparagine
Glycosylation175N-linked (GlcNAc...) asparagine
Glycosylation187N-linked (GlcNAc...) asparagine

Post-translational modification

N-glycosylated.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Ubiquitously expressed.

Developmental stage

Expressed in neonatal brain and in day 10 and 13 embryo.

Gene expression databases

Interaction

Protein-protein interaction databases

Miscellaneous

Family & Domains

Sequence similarities

Belongs to the PC-esterase family. CASD1 subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    797
  • Mass (Da)
    91,603
  • Last updated
    2003-10-01 v1
  • Checksum
    9FF23144DDDA8741
MAALAYNLGKREINHYFSVRSAKVLALVAVLLLAACHLASRRYRGNDSCEYLLSSGRFLGEKVWQPHSCMMHKYKISEAKTCLVDKHIAFIGDSRIRQLFYSFVKIINPQFKEEGNKHENIPFEDKAASVKVDFLWHPEVNGSMKQCIKVWTEDSVLKPHVIVAGAATWSIKIHNGSEEALAQYKMNITSIAPLLEKLAKTSDVYWVLQDPVYEDLLSENRKMITNEKIDAYNEAAVSILNSSTRTSKSNVKMFSVSKLIAQETIMESLDGLHLPESSRETSAMILMNVYCNKVVKPVDGSCCQPRPPLTLIQKLAACFFTLSIIGYFIFYVIHRNAHRKNKPCTDLESGEEKKNIINTPVSSLEILLQSFCKLGLIMAYFYMCDRANLFMKENKFYTHSSFFIPIIYILVLGVFYNENTKETKVLNREQTDEWKGWMQLVILIYHISGASTFLPVYMHIRVLVAAYLFQTGYGHFSYFWIKGDFGIHRVCQVLFRLNFLVVVLCIVMDRPYQFYYFVPLVTVWFMVIYVTLALWPQITQKKANGNFFWYLGLLLKLGLLLLCIWFLAYSQGAFEKIFSLWPLSKCFELEGSVYEWWFRWRLDRYVVFHGVLFAFIYLALQRRQILSEGKGEPLFSNKISNFLLFVSVVSFLTYSIWASSCKNKAECNELHPSVSVVQIVAFILIRNIPGYARSIYSSFFAWFGKISLELFICQYHIWLAADTRGILVLIPGNPTLNIIVSTFIFVCVAHEISQITTDLAQVVIPKDNPSLFRRLACTIAFFGGVLILSSIQDKSRL

Computationally mapped potential isoform sequences

There are 4 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
M0QWG8M0QWG8_MOUSECasd1220
A0A0N4SWB0A0A0N4SWB0_MOUSECasd171
A0A0N4SW49A0A0N4SW49_MOUSECasd1132
F6TUA2F6TUA2_MOUSECasd1182

Sequence caution

The sequence AAH18542.1 differs from that shown. Reason: Erroneous initiation
The sequence AAH38009.1 differs from that shown. Reason: Erroneous initiation

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB091829
EMBL· GenBank· DDBJ
BAC77246.1
EMBL· GenBank· DDBJ
mRNA
BC018542
EMBL· GenBank· DDBJ
AAH18542.1
EMBL· GenBank· DDBJ
mRNA Different initiation
BC038009
EMBL· GenBank· DDBJ
AAH38009.1
EMBL· GenBank· DDBJ
mRNA Different initiation
BC058953
EMBL· GenBank· DDBJ
AAH58953.1
EMBL· GenBank· DDBJ
mRNA
BC125377
EMBL· GenBank· DDBJ
AAI25378.1
EMBL· GenBank· DDBJ
mRNA
BC125379
EMBL· GenBank· DDBJ
AAI25380.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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