Q7TMY7 · IPO8_MOUSE
- ProteinImportin-8
- GeneIpo8
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1010 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, may serve as receptor for nuclear localization signals (NLS) and promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro mediates the nuclear import of the signal recognition particle protein SRP19. May also be involved in cytoplasm-to-nucleus shuttling of a broad spectrum of other cargos, including Argonaute-microRNAs complexes, the JUN protein, RELA/NF-kappa-B p65 subunit, the translation initiation factor EIF4E and a set of receptor-activated mothers against decapentaplegic homolog (SMAD) transcription factors that play a critical role downstream of the large family of transforming growth factor beta and bone morphogenetic protein (BMP) cytokines.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Cellular Component | plasma membrane | |
Molecular Function | ATPase-coupled transmembrane transporter activity | |
Molecular Function | small GTPase binding | |
Biological Process | intracellular protein transport | |
Biological Process | nucleocytoplasmic transport |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameImportin-8
- Short namesImp8
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ7TMY7
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Disruption phenotype
Knockout animals present with reduced grip strength and diminished vertical activity. Progressive dilatation can be observed in the aortic root at the level of the sinuses of Valsalva and distal ascending aorta, and aneurysms of the distal ascending aorta are becoming visible at the age of 8-12 weeks. Generally, males are more severely affected, exhibiting larger aortas and experiencing dissection and/or rupture more frequently than females.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 47 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000120753 | 1-1010 | Importin-8 | ||
Modified residue | 902 | Phosphoserine | |||
Modified residue | 903 | Phosphoserine | |||
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Developmental stage
Abundantly expressed in secretory and mature ameloblasts and weakly expressed in pre-ameloblasts of the lower incisor and molar at birth (at protein level).
Gene expression databases
Interaction
Subunit
Forms a heterodimer with KPNB1. Interacts with SRP19. Interacts with RPL23A. Binds directly to nuclear pore complexes. Interacts with LRPPRC; the interaction occurs when LRPPRC is in its RNA-free form and promotes import of LRPPRC to the nucleus to allow for EIF4E-mediated export of mRNAS from the nucleus to the cytoplasm.
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 22-102 | Importin N-terminal | |||
Region | 886-932 | Disordered | |||
Sequence similarities
Belongs to the importin beta family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q7TMY7-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length1,010
- Mass (Da)117,078
- Last updated2011-07-27 v3
- MD5 Checksum45C0388B95842D8196ABAB8661CC0E48
Q7TMY7-2
- Name2
- Differences from canonical
- 304-347: Missing
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A5F8MQ75 | A0A5F8MQ75_MOUSE | Ipo8 | 513 | ||
A0A0N4SUM9 | A0A0N4SUM9_MOUSE | Ipo8 | 35 | ||
B2KGD7 | B2KGD7_MOUSE | Ipo8 | 361 |
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Alternative sequence | VSP_009656 | 304-347 | in isoform 2 | ||
Sequence conflict | 405 | in Ref. 3; AAH44819/AAH54373 | |||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CU302294 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH466572 EMBL· GenBank· DDBJ | EDL10748.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC024474 EMBL· GenBank· DDBJ | AAH24474.1 EMBL· GenBank· DDBJ | mRNA | ||
BC027360 EMBL· GenBank· DDBJ | AAH27360.1 EMBL· GenBank· DDBJ | mRNA | ||
BC044819 EMBL· GenBank· DDBJ | AAH44819.1 EMBL· GenBank· DDBJ | mRNA | ||
BC054373 EMBL· GenBank· DDBJ | AAH54373.1 EMBL· GenBank· DDBJ | mRNA | ||
BC151036 EMBL· GenBank· DDBJ | AAI51037.1 EMBL· GenBank· DDBJ | mRNA | ||
BC151052 EMBL· GenBank· DDBJ | AAI51053.1 EMBL· GenBank· DDBJ | mRNA | ||
AK047854 EMBL· GenBank· DDBJ | BAC33175.1 EMBL· GenBank· DDBJ | mRNA | ||
CD350256 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
BQ770006 EMBL· GenBank· DDBJ | - | mRNA | No translation available. |