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Q7TMY7 · IPO8_MOUSE

  • Protein
    Importin-8
  • Gene
    Ipo8
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Involved in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, may serve as receptor for nuclear localization signals (NLS) and promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro mediates the nuclear import of the signal recognition particle protein SRP19. May also be involved in cytoplasm-to-nucleus shuttling of a broad spectrum of other cargos, including Argonaute-microRNAs complexes, the JUN protein, RELA/NF-kappa-B p65 subunit, the translation initiation factor EIF4E and a set of receptor-activated mothers against decapentaplegic homolog (SMAD) transcription factors that play a critical role downstream of the large family of transforming growth factor beta and bone morphogenetic protein (BMP) cytokines.

Caution

The regions from 407 to 653 were deduced from the genomic sequence and ESTs by similarity to the human sequence.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleus
Cellular Componentplasma membrane
Molecular FunctionATPase-coupled transmembrane transporter activity
Molecular Functionsmall GTPase binding
Biological Processintracellular protein transport
Biological Processnucleocytoplasmic transport

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Importin-8
  • Short names
    Imp8
  • Alternative names
    • Ran-binding protein 8 (RanBP8
      )

Gene names

    • Name
      Ipo8

Organism names

  • Taxonomic identifier
  • Strains
    • C57BL/6J
    • FVB/N
    • FVB/N-3
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q7TMY7
  • Secondary accessions
    • B2KGD8
    • Q811I3
    • Q8C8A9
    • Q8R2P6
    • Q8R3V7

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Knockout animals present with reduced grip strength and diminished vertical activity. Progressive dilatation can be observed in the aortic root at the level of the sinuses of Valsalva and distal ascending aorta, and aneurysms of the distal ascending aorta are becoming visible at the age of 8-12 weeks. Generally, males are more severely affected, exhibiting larger aortas and experiencing dissection and/or rupture more frequently than females.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 47 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

Type
IDPosition(s)Description
ChainPRO_00001207531-1010Importin-8
Modified residue902Phosphoserine
Modified residue903Phosphoserine

Keywords

Proteomic databases

PTM databases

Expression

Developmental stage

Abundantly expressed in secretory and mature ameloblasts and weakly expressed in pre-ameloblasts of the lower incisor and molar at birth (at protein level).

Gene expression databases

Interaction

Subunit

Forms a heterodimer with KPNB1. Interacts with SRP19. Interacts with RPL23A. Binds directly to nuclear pore complexes. Interacts with LRPPRC; the interaction occurs when LRPPRC is in its RNA-free form and promotes import of LRPPRC to the nucleus to allow for EIF4E-mediated export of mRNAS from the nucleus to the cytoplasm.

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain22-102Importin N-terminal
Region886-932Disordered

Sequence similarities

Belongs to the importin beta family.

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q7TMY7-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    1,010
  • Mass (Da)
    117,078
  • Last updated
    2011-07-27 v3
  • MD5 Checksum
    45C0388B95842D8196ABAB8661CC0E48
MDLNRIIQALKGTIDPKLRIAAETELNQSYKIINFAPSLLRIIVSDHVEFPVRQAAAIYLKNMVTQYWPDREPPPGEVIFPFNIHENDRQQIRDNIVEGIIRSPDLVRVQLTMCLRVIIRHDFPGHWPAVVDKIDYYLQSPNSGSWLGSLLCLYQLVKTYEYKKAEEREPLLAAMQIFLPRIQQQILQLLPDASHYSVLLQKQILKIFYALVQYALPLQLVNHQTMTTWMEIFRTIIDRTVPPETLQIDEDDRPELVWWKCKKWALHIVARLFERYGSPGNVTKEYFEFSEFFLKTYAVGIQQVLLKILDQYRQKEYIAPRVLQQAFNYLNQGVVHAVTWKQMKPHIQNISEDVIFSVMCYKDEDEELWQEDPYEYIRMKFDIFEDYASPTTAAQTLLYTAAKKRKEVLPKMMAFCYQILTDPNFDPRKKDGALHVIGSLAEILLKKSLFKDQIELFLQNHVFPLLMSNLGYLRARSCWVLHAFSSLKFHNELNLRNAVELAKKSLIEDEEMPVKVEAALALQSLISNQAQAKEHMKPYVRFIMQELLHIVRETENDDVTNVIQKLICEYSQDVASIAVDTTQHLAEIFGKVLQSDEYEEIEDKTVMAMGILHTIDTILTVVEDHPEIIQQLENICLRIIDLVLQKHVIEFYEEILSLAYNLTCHTISPQMWQLLGILYEVFQQDCFEYFTDMMPLLHNYVTVDTNALLSNPKHLEVLFTMCRKVLCGEAGEDAECYAAKLLEVIILQCKGRGIDQCIPLFIQLVLERLTRGVKTSELRTMCLQVAIAALYYSPELLFHTLEQVQLPHNPGPVTSQFINQWMNDTDYFLGHHDRKMCIIGLSVLLELQNRPPAVDAVAAQILPSILFLFLGLKQVCATRQTVNRENHSKAEKVDIEENEEISSEEEEETSVSAQAMQSQIGRSEEEDDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALLTVQNRDAAWYQLLVAPLSEDQKRKLQEVYTLAEHRRTLAAGQFHI

Q7TMY7-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A5F8MQ75A0A5F8MQ75_MOUSEIpo8513
A0A0N4SUM9A0A0N4SUM9_MOUSEIpo835
B2KGD7B2KGD7_MOUSEIpo8361

Features

Showing features for alternative sequence, sequence conflict.

Type
IDPosition(s)Description
Alternative sequenceVSP_009656304-347in isoform 2
Sequence conflict405in Ref. 3; AAH44819/AAH54373

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CU302294
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CH466572
EMBL· GenBank· DDBJ
EDL10748.1
EMBL· GenBank· DDBJ
Genomic DNA
BC024474
EMBL· GenBank· DDBJ
AAH24474.1
EMBL· GenBank· DDBJ
mRNA
BC027360
EMBL· GenBank· DDBJ
AAH27360.1
EMBL· GenBank· DDBJ
mRNA
BC044819
EMBL· GenBank· DDBJ
AAH44819.1
EMBL· GenBank· DDBJ
mRNA
BC054373
EMBL· GenBank· DDBJ
AAH54373.1
EMBL· GenBank· DDBJ
mRNA
BC151036
EMBL· GenBank· DDBJ
AAI51037.1
EMBL· GenBank· DDBJ
mRNA
BC151052
EMBL· GenBank· DDBJ
AAI51053.1
EMBL· GenBank· DDBJ
mRNA
AK047854
EMBL· GenBank· DDBJ
BAC33175.1
EMBL· GenBank· DDBJ
mRNA
CD350256
EMBL· GenBank· DDBJ
-mRNA No translation available.
BQ770006
EMBL· GenBank· DDBJ
-mRNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

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