Q7SZM5 · Q7SZM5_DANRE
- Protein72 kDa type IV collagenase
- Genemmp2
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids657 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
Catalytic activity
Cofactor
Protein has several cofactor binding sites:
Note: Can bind about 5 Ca2+ ions per subunit.
Note: Binds 2 Zn2+ ions per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 102 | Zn2+ 2 (UniProtKB | ChEBI); catalytic; in inhibited form | ||||
Sequence: C | ||||||
Binding site | 134 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 168 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 178 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 180 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 185 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 186 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 190 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 193 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 202 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 204 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 206 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 208 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 209 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 211 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 211 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 231 | |||||
Sequence: E | ||||||
Active site | 404 | |||||
Sequence: E | ||||||
Binding site | 473 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 518 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 520 | Ca2+ 5 (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 568 | Ca2+ 5 (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 615 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | extracellular matrix | |
Cellular Component | extracellular space | |
Cellular Component | sarcomere | |
Molecular Function | metalloendopeptidase activity | |
Molecular Function | zinc ion binding | |
Biological Process | collagen catabolic process | |
Biological Process | extracellular matrix organization | |
Biological Process | hematopoietic stem cell migration | |
Biological Process | lymph vessel development | |
Biological Process | positive regulation of transforming growth factor beta receptor signaling pathway | |
Biological Process | proteolysis | |
Biological Process | response to hypoxia | |
Biological Process | retinal ganglion cell axon guidance | |
Biological Process | tissue remodeling |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended name72 kDa type IV collagenase
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio
Accessions
- Primary accessionQ7SZM5
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for signal, chain, disulfide bond, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-29 | |||||
Sequence: MLSVKFFRCRHIVLKVFLVQFLASLQTFA | ||||||
Chain | PRO_5035035830 | 30-657 | 72 kDa type IV collagenase | |||
Sequence: APSPIIKFPGDDTAHTDKEVALHYLNKFYGCPKDRCNLMVLKDTLKKMQKFFALPETGEIDQKTVEIMKKPRCGVPDVANYNFFHRKPKWGQKNVTYRILGHTPDLDEDTIDDAFYRAFKVWSDVTPLKFTRIMDGEADIMINFGRNEHGDGYPFDGKDGLLAHAFAPGPGIGGDSHFDDDEQWTLGEGQVVKVKYGNAEGEFCKFPFLFMGKEYNSCTSQGRDDGFLWCSTTYNFDDDGKYGFCPHELLFTLGGNADGAPCKFPFTFQGDKYDSCTTSGRDDGYRWCATTEDYDKDKTYGFCPETAMSTSGGNSDGAPCVFPFKFLGDSYDSCTTSGRNDGKMWCAVTKSFDDDRKWGFCPDQGYSLFLVAAHEFGHALGLEHSDDPGALMAPIYTFTKTLRLSDDDVKGIQELYGEPTDKPLATHTPPVTPMDVCNENIIFDAVAQIRGEIFFFKDRFLFRTADVRKKPTGPMLVATFWSELPEKIDAAYENPLEERTVFFAGDEMWVYSASTLEREYPKKISSMGLPSDLHGIDAAYSFHKTKKTYIFAGNKFWRYNEAKKKMDPGFPKIIADSWTAVPDDLDGALSLNGDGHSYFFKDSHYLKMDDSTLKIIKVGEVKKDWLRC | ||||||
Disulfide bond | 233↔259 | |||||
Sequence: CKFPFLFMGKEYNSCTSQGRDDGFLWC | ||||||
Disulfide bond | 247↔274 | |||||
Sequence: CTSQGRDDGFLWCSTTYNFDDDGKYGFC | ||||||
Disulfide bond | 291↔317 | |||||
Sequence: CKFPFTFQGDKYDSCTTSGRDDGYRWC | ||||||
Disulfide bond | 305↔332 | |||||
Sequence: CTTSGRDDGYRWCATTEDYDKDKTYGFC | ||||||
Disulfide bond | 349↔375 | |||||
Sequence: CVFPFKFLGDSYDSCTTSGRNDGKMWC | ||||||
Disulfide bond | 363↔390 | |||||
Sequence: CTTSGRNDGKMWCAVTKSFDDDRKWGFC | ||||||
Disulfide bond | 466↔657 | |||||
Sequence: CNENIIFDAVAQIRGEIFFFKDRFLFRTADVRKKPTGPMLVATFWSELPEKIDAAYENPLEERTVFFAGDEMWVYSASTLEREYPKKISSMGLPSDLHGIDAAYSFHKTKKTYIFAGNKFWRYNEAKKKMDPGFPKIIADSWTAVPDDLDGALSLNGDGHSYFFKDSHYLKMDDSTLKIIKVGEVKKDWLRC | ||||||
Modified residue | 549 | Phosphotyrosine; by PKDCC | ||||
Sequence: Y |
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for motif, domain, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 100-107 | Cysteine switch | ||||
Sequence: PRCGVPDV | ||||||
Domain | 228-276 | Fibronectin type-II | ||||
Sequence: AEGEFCKFPFLFMGKEYNSCTSQGRDDGFLWCSTTYNFDDDGKYGFCPH | ||||||
Domain | 286-334 | Fibronectin type-II | ||||
Sequence: ADGAPCKFPFTFQGDKYDSCTTSGRDDGYRWCATTEDYDKDKTYGFCPE | ||||||
Domain | 344-392 | Fibronectin type-II | ||||
Sequence: SDGAPCVFPFKFLGDSYDSCTTSGRNDGKMWCAVTKSFDDDRKWGFCPD | ||||||
Repeat | 469-513 | Hemopexin | ||||
Sequence: NIIFDAVAQIRGEIFFFKDRFLFRTADVRKKPTGPMLVATFWSEL | ||||||
Repeat | 514-560 | Hemopexin | ||||
Sequence: PEKIDAAYENPLEERTVFFAGDEMWVYSASTLEREYPKKISSMGLPS | ||||||
Repeat | 562-610 | Hemopexin | ||||
Sequence: LHGIDAAYSFHKTKKTYIFAGNKFWRYNEAKKKMDPGFPKIIADSWTAV | ||||||
Repeat | 611-657 | Hemopexin | ||||
Sequence: PDDLDGALSLNGDGHSYFFKDSHYLKMDDSTLKIIKVGEVKKDWLRC |
Sequence similarities
Belongs to the peptidase M10A family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length657
- Mass (Da)74,276
- Last updated2003-10-01 v1
- Checksum09E4CC660B46B9EA
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY324195 EMBL· GenBank· DDBJ | AAP74482.1 EMBL· GenBank· DDBJ | mRNA |