Q7SXW4 · EMC3_DANRE
- ProteinER membrane protein complex subunit 3
- Geneemc3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids261 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | EMC complex | |
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | Golgi apparatus | |
Cellular Component | membrane | |
Cellular Component | trans-Golgi network transport vesicle | |
Biological Process | photoreceptor cell maintenance | |
Biological Process | protein insertion into ER membrane by stop-transfer membrane-anchor sequence | |
Biological Process | retina development in camera-type eye | |
Biological Process | tail-anchored membrane protein insertion into ER membrane |
Names & Taxonomy
Protein names
- Recommended nameER membrane protein complex subunit 3
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio
Accessions
- Primary accessionQ7SXW4
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-14 | Lumenal | ||||
Sequence: MAEPELLLDSNIRL | ||||||
Transmembrane | 15-38 | Helical | ||||
Sequence: WVVLPIVFITFLVGVIRHYVSILL | ||||||
Topological domain | 39-114 | Cytoplasmic | ||||
Sequence: QSDKKLTLEQVSDSQVLIRSRVLRENGKYIPKQSFLMRKFYFNNQEDGFFKKTKRKVVPPSPMTDPSMLTDMMKGN | ||||||
Transmembrane | 115-130 | Helical | ||||
Sequence: VTNVLPMILIGGWINW | ||||||
Topological domain | 131-168 | Lumenal | ||||
Sequence: TFSGFVTTKVPFPLTLRFKPMLQQGIELLSLDASWVSS | ||||||
Transmembrane | 169-187 | Helical | ||||
Sequence: ASWYFLNVFGLRSMYSLIL | ||||||
Topological domain | 188-261 | Cytoplasmic | ||||
Sequence: GQDNGADQSRIMQEQMSGAAMAMPADTNKAFKAEWEALELTDHQWALENVEEDLMSKDLDLSGMFSKDLPTGIF |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000211410 | 1-261 | ER membrane protein complex subunit 3 | |||
Sequence: MAEPELLLDSNIRLWVVLPIVFITFLVGVIRHYVSILLQSDKKLTLEQVSDSQVLIRSRVLRENGKYIPKQSFLMRKFYFNNQEDGFFKKTKRKVVPPSPMTDPSMLTDMMKGNVTNVLPMILIGGWINWTFSGFVTTKVPFPLTLRFKPMLQQGIELLSLDASWVSSASWYFLNVFGLRSMYSLILGQDNGADQSRIMQEQMSGAAMAMPADTNKAFKAEWEALELTDHQWALENVEEDLMSKDLDLSGMFSKDLPTGIF |
Proteomic databases
Expression
Tissue specificity
Localized to the inner segment and synapse in photoreceptor cells.
Gene expression databases
Interaction
Subunit
Component of the ER membrane protein complex (EMC).
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length261
- Mass (Da)29,792
- Last updated2003-10-01 v1
- ChecksumEC717145BD15EEBC
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 25 | in Ref. 1; AAW19648 | ||||
Sequence: F → I |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY745978 EMBL· GenBank· DDBJ | AAW19648.1 EMBL· GenBank· DDBJ | mRNA | ||
AY398377 EMBL· GenBank· DDBJ | AAQ97810.1 EMBL· GenBank· DDBJ | mRNA | ||
BC055222 EMBL· GenBank· DDBJ | AAH55222.1 EMBL· GenBank· DDBJ | mRNA |