Q7SIF4 · AIOA_ALCFA
- ProteinArsenite oxidase subunit AioA
- GeneaioA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids826 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the detoxification of arsenic. Oxidizes As(III)O33- (arsenite) to the somewhat less toxic As(V)O43- (arsenate).
Catalytic activity
- arsenite + H2O + 2 oxidized [azurin] = arsenate + 3 H+ + 2 reduced [azurin]
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 [3Fe-4S] cluster per subunit.
Note: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 22 | [3Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 25 | [3Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 29 | [3Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Site | 100 | Involved in charge transfer | ||||
Sequence: S | ||||||
Binding site | 196 | substrate | ||||
Sequence: H | ||||||
Binding site | 204 | substrate | ||||
Sequence: E | ||||||
Binding site | 420 | substrate | ||||
Sequence: R | ||||||
Binding site | 424 | substrate | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | oxidoreductase complex | |
Molecular Function | 3 iron, 4 sulfur cluster binding | |
Molecular Function | arsenate reductase (azurin) activity | |
Molecular Function | metal ion binding | |
Molecular Function | molybdopterin cofactor binding | |
Biological Process | cellular respiration |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameArsenite oxidase subunit AioA
- EC number
- Short namesAOI
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Alcaligenaceae > Alcaligenes
Accessions
- Primary accessionQ7SIF4
- Secondary accessions
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000063240 | 2-826 | Arsenite oxidase subunit AioA | |||
Sequence: SRPNDRITLPPANAQRTNMTCHFCIVGCGYHVYKWPELQEGGRAPEQNALGLDFRKQLPPLAVTLTPAMTNVVTEHNGRRYNIMVVPDKACVVNSGLSSTRGGKMASYMYTPTGDGKQRLKAPRLYAADQWVDTTWDHAMALYAGLIKKTLDKDGPQGVFFSCFDHGGAGGGFENTWGTGKLMFSAIQTPMVRIHNRPAYNSECHATREMGIGELNNAYEDAQLADVIWSIGNNPYESQTNYFLNHWLPNLQGATTSKKKERFPNENFPQARIIFVDPRETPSVAIARHVAGNDRVLHLAIEPGTDTALFNGLFTYVVEQGWIDKPFIEAHTKGFDDAVKTNRLSLDECSNITGVPVDMLKRAAEWSYKPKASGQAPRTMHAYEKGIIWGNDNYVIQSALLDLVIATHNVGRRGTGCVRMGGHQEGYTRPPYPGDKKIYIDQELIKGKGRIMTWWGCNNFQTSNNAQALREAILQRSAIVKQAMQKARGATTEEMVDVIYEATQNGGLFVTSINLYPTKLAEAAHLMLPAAHPGEMNLTSMNGERRIRLSEKFMDPPGTAMADCLIAARIANALRDMYQKDGKAEMAAQFEGFDWKTEEDAFNDGFRRAGQPGAPAIDSQGGSTGHLVTYDRLRKSGNNGVQLPVVSWDESKGLVGTEMLYTEGKFDTDDGKAHFKPAPWNGLPATVQQQKDKYRFWLNNGRNNEVWQTAYHDQYNSLMQERYPMAYIEMNPDDCKQLDVTGGDIVEVYNDFGSTFAMVYPVAEIKRGQTFMLFGYVNGIQGDVTTDWTDRNIIPYYKGTWGDIRKVGSMEEFKRTVSFKSRRFA |
Interaction
Subunit
Heterodimer consisting of a large and a small subunit.
Structure
Sequence
- Sequence statusComplete
- Length826
- Mass (Da)92,395
- Last updated2012-01-25 v2
- Checksum56AFA94C70E82575
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 2-3 | in Ref. 2; AA sequence and 3; AA sequence | ||||
Sequence: SR → GC | ||||||
Sequence conflict | 40 | in Ref. 2; AA sequence | ||||
Sequence: Q → E | ||||||
Sequence conflict | 64 | in Ref. 2; AA sequence | ||||
Sequence: V → S | ||||||
Sequence conflict | 78-83 | in Ref. 2; AA sequence | ||||
Sequence: NGRRYN → DGARYD | ||||||
Sequence conflict | 119 | in Ref. 2; AA sequence | ||||
Sequence: Q → E | ||||||
Sequence conflict | 122 | in Ref. 2; AA sequence | ||||
Sequence: K → S | ||||||
Sequence conflict | 131 | in Ref. 2; AA sequence | ||||
Sequence: Q → E | ||||||
Sequence conflict | 154 | in Ref. 2; AA sequence | ||||
Sequence: K → S | ||||||
Sequence conflict | 793 | in Ref. 2; AA sequence | ||||
Sequence: N → D | ||||||
Sequence conflict | 812 | in Ref. 2; AA sequence | ||||
Sequence: E → S | ||||||
Sequence conflict | 826 | in Ref. 2; AA sequence | ||||
Sequence: A → G |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY297781 EMBL· GenBank· DDBJ | AAQ19838.1 EMBL· GenBank· DDBJ | Genomic DNA |