Q7NHF0 · Q7NHF0_GLOVI
- ProteinGlutamine-dependent NAD(+) synthetase
- GenenadE
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids540 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.
Catalytic activity
- deamido-NAD+ + L-glutamine + ATP + H2O = L-glutamate + AMP + diphosphate + NAD+ + H+
Pathway
Cofactor biosynthesis; NAD+ biosynthesis; NAD+ from deamido-NAD+ (L-Gln route): step 1/1.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 41 | Proton acceptor; for glutaminase activity | |||
Active site | 111 | For glutaminase activity | |||
Binding site | 117 | L-glutamine (UniProtKB | ChEBI) | |||
Active site | 147 | Nucleophile; for glutaminase activity | |||
Binding site | 184 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 190 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 292-299 | ATP (UniProtKB | ChEBI) | |||
Binding site | 375 | deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits | |||
Binding site | 399 | ATP (UniProtKB | ChEBI) | |||
Binding site | 404 | deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits | |||
Binding site | 514 | deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | glutaminase activity | |
Molecular Function | NAD+ synthase (glutamine-hydrolyzing) activity | |
Molecular Function | NAD+ synthase activity | |
Biological Process | NAD biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamine-dependent NAD(+) synthetase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillati > Cyanobacteriota > Cyanophyceae > Gloeobacterales > Gloeobacteraceae > Gloeobacter
Accessions
- Primary accessionQ7NHF0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-254 | CN hydrolase | |||
Sequence similarities
Belongs to the NAD synthetase family.
In the C-terminal section; belongs to the NAD synthetase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length540
- Mass (Da)58,271
- Last updated2003-12-15 v1
- MD5 Checksum6CA6F20EDA91202883959E9718A4E764
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BA000045 EMBL· GenBank· DDBJ | BAC90528.1 EMBL· GenBank· DDBJ | Genomic DNA |