Q7NE65 · MIAB_GLOVI
- ProteintRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
- GenemiaB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids441 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.
Catalytic activity
- N6-dimethylallyladenosine37 in tRNA + [sulfur carrier]-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N6-dimethylallyladenosine37 in tRNA + [sulfur carrier]-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H+
Cofactor
Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 19 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 55 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 89 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 161 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 165 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 168 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | methylthiotransferase activity | |
Biological Process | tRNA methylthiolation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Gloeobacterales > Gloeobacteraceae > Gloeobacter
Accessions
- Primary accessionQ7NE65
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000374322 | 1-441 | tRNA-2-methylthio-N6-dimethylallyladenosine synthase | ||
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 10-126 | MTTase N-terminal | |||
Domain | 147-375 | Radical SAM core | |||
Domain | 378-441 | TRAM | |||
Sequence similarities
Belongs to the methylthiotransferase family. MiaB subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length441
- Mass (Da)49,263
- Last updated2003-12-15 v1
- Checksum6E9ED377DFB4AF91
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BA000045 EMBL· GenBank· DDBJ | BAC91956.1 EMBL· GenBank· DDBJ | Genomic DNA |