Essential maintenance is planned to begin on Fri Jan 24 2025. The website may be temporarily unavailable. Please use our fallback: https://wwwdev.ebi.ac.uk/uniprot/front-end/fallback/ in case of any outage.

Q7NCE3 · QUEE_GLOVI

Function

function

Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
S-adenosyl-L-methionine (UniProtKB | Rhea| CHEBI:59789 )

Note: Binds 1 S-adenosyl-L-methionine per subunit.
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site26-28substrate
Binding site41substrate
Binding site45[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site49[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site52[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site54Mg2+ (UniProtKB | ChEBI)
Binding site85substrate
Binding site87S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site130-132S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site214substrate

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functioncarbon-nitrogen lyase activity
Molecular Functionmagnesium ion binding
Molecular FunctionS-adenosyl-L-methionine binding
Biological Processqueuosine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    7-carboxy-7-deazaguanine synthase
  • EC number
  • Short names
    CDG synthase
  • Alternative names
    • Queuosine biosynthesis protein QueE

Gene names

    • Name
      queE
    • Ordered locus names
      gll3036

Organism names

Accessions

  • Primary accession
    Q7NCE3

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004162061-2147-carboxy-7-deazaguanine synthase

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain32-214Radical SAM core

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    214
  • Mass (Da)
    23,573
  • Last updated
    2003-12-15 v1
  • MD5 Checksum
    9C60CE0C8C5D4D53D73C85288F249828
MNAASTVVKPVSAQSIPVQETFGPTIQGEGYWAGAVVDFIRLYGCPVGCPWCDTGYADGGPQLPRAVRTFSDLILELKSPRVVISGGEPFIHAALPALVEAIGRTGRAVAIETSGAFWQPVPDWAWVTLSPKEHVSPRHPVNPQMWRRANEVKIVIAGGAELDVYRRFLPPGVPVSLQPEWEARERTLPLTLELLKAHPRYRLSVQLHKYLQVP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BA000045
EMBL· GenBank· DDBJ
BAC90977.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help