Q7NCE3 · QUEE_GLOVI
- Protein7-carboxy-7-deazaguanine synthase
- GenequeE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids214 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.
Catalytic activity
- 6-carboxy-5,6,7,8-tetrahydropterin + H+ = 7-carboxy-7-deazaguanine + NH4+
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Note: Binds 1 S-adenosyl-L-methionine per subunit.
Pathway
Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 26-28 | substrate | |||
Binding site | 41 | substrate | |||
Binding site | 45 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 49 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 52 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 54 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 85 | substrate | |||
Binding site | 87 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 130-132 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 214 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | carbon-nitrogen lyase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | S-adenosyl-L-methionine binding | |
Biological Process | queuosine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name7-carboxy-7-deazaguanine synthase
- EC number
- Short namesCDG synthase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Gloeobacterales > Gloeobacteraceae > Gloeobacter
Accessions
- Primary accessionQ7NCE3
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000416206 | 1-214 | 7-carboxy-7-deazaguanine synthase | ||
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 32-214 | Radical SAM core | |||
Sequence similarities
Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length214
- Mass (Da)23,573
- Last updated2003-12-15 v1
- MD5 Checksum9C60CE0C8C5D4D53D73C85288F249828
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BA000045 EMBL· GenBank· DDBJ | BAC90977.1 EMBL· GenBank· DDBJ | Genomic DNA |