Q7N8W2 · CARA_PHOLL

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site47L-glutamine (UniProtKB | ChEBI)
Binding site241L-glutamine (UniProtKB | ChEBI)
Binding site243L-glutamine (UniProtKB | ChEBI)
Active site269Nucleophile
Binding site270L-glutamine (UniProtKB | ChEBI)
Binding site273L-glutamine (UniProtKB | ChEBI)
Binding site311L-glutamine (UniProtKB | ChEBI)
Binding site313L-glutamine (UniProtKB | ChEBI)
Binding site314L-glutamine (UniProtKB | ChEBI)
Active site353
Active site355

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • Ordered locus names
      plu0603

Organism names

Accessions

  • Primary accession
    Q7N8W2

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001123021-387Carbamoyl phosphate synthase small chain

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-189CPSase
Domain193-380Glutamine amidotransferase type-1

Sequence similarities

Belongs to the CarA family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    387
  • Mass (Da)
    42,415
  • Last updated
    2003-12-15 v1
  • Checksum
    F8756B2AC2E5F768
MIKSAILVLEDGTQFHGRAIGAEGAAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGVNSVDKESLKVQAQGLVIRDLPLLTSNFRCEETLSDYLKRHNIVAIADIDTRKLTRLLREKGSQNGCIIAGKQIDAQVALEKAQAFPGLEGMDLAKEVTTKQIYPWLQGSWKLAGGLPEDKQEQDLPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTSAEDVLKLNPDGIFLSNGPGDPAPCGYAIDAVKTLLETEIPIFGICLGHQLLALASGAETMKMKFGHHGGNHPVKDLECNVVMITAQNHGFAVDEKSLPSNLRVTHKSLFDGTLQGIHRTDKPAFSFQGHPEASPGPHETASLFDHFIELIEQYCQKNNRHNTK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BX571860
EMBL· GenBank· DDBJ
CAE12898.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp