Q7N8U4 · TYSY_PHOLL
- ProteinThymidylate synthase
- GenethyA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids264 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.
Catalytic activity
- dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP
Pathway
Pyrimidine metabolism; dTTP biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 21 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 51 | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
Binding site | 126-127 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
Active site | 146 | Nucleophile | |||
Binding site | 166-169 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 169 | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
Binding site | 177 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 207-209 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | |||
Binding site | 263 | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | thymidylate synthase activity | |
Biological Process | dTMP biosynthetic process | |
Biological Process | dTTP biosynthetic process | |
Biological Process | methylation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThymidylate synthase
- EC number
- Short namesTS ; TSase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Morganellaceae > Photorhabdus
Accessions
- Primary accessionQ7N8U4
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000141000 | 1-264 | Thymidylate synthase | ||
Interaction
Structure
Sequence
- Sequence statusComplete
- Length264
- Mass (Da)30,184
- Last updated2003-12-15 v1
- ChecksumDE15A59A386CB1C6
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX571861 EMBL· GenBank· DDBJ | CAE12918.1 EMBL· GenBank· DDBJ | Genomic DNA |